Calreticulin 3 (CALR3) (NM_145046) Human Tagged ORF Clone

CAT#: RC205398

CALR3 (Myc-DDK-tagged)-Human calreticulin 3 (CALR3)

Product Images

Western blot validation of overexpression lysate (Cat# LY403418) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205398 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CALR3 protein (Cat# TP305398). The protein was produced from HEK293T cells transfected with CALR3 cDNA clone (Cat# RC205398) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CALR3
• Synonyms: CMH19; CRT2; CT93
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205398 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGCTTTGGTCCAGTTCTGGGCCATATGCATGCTGCGAGTGGCGCTGGCTACCGTCTATTTCC
  AAGAGGAATTTCTAGACGGAGAGCATTGGAGAAACCGATGGTTGCAGTCCACCAATGACTCCCGATTTGG
  GCATTTTAGACTTTCGTCGGGCAAGTTTTATGGTCATAAAGAGAAAGATAAAGGTCTGCAAACCACTCAG
  AATGGCCGATTCTATGCCATCTCTGCACGCTTCAAACCGTTCAGCAATAAAGGGAAAACTCTGGTTATTC
  AGTACACAGTAAAACATGAGCAGAAGATGGACTGTGGAGGGGGCTACATTAAGGTCTTTCCTGCAGACAT
  TGACCAGAAGAACCTGAATGGAAAATCGCAATACTATATTATGTTTGGACCCGATATTTGTGGATTTGAT
  ATCAAGAAAGTTCATGTTATTTTACATTTCAAGAATAAGTATCACGAAAACAAGAAACTGATCAGGTGTA
  AGGTTGATGGCTTCACACACCTGTACACTCTAATTTTAAGACCAGATCTTTCTTATGATGTGAAAATTGA
  TGGTCAGTCAATTGAATCCGGCAGCATAGAGTACGACTGGAACTTAACATCACTCAAGAAGGAAACGTCC
  CCGGCAGAATCGAAGGATTGGGAACAGACTAAAGACAACAAAGCCCAGGACTGGGAGAAGCATTTTCTGG
  ACGCCAGCACCAGCAAGCAGAGCGACTGGAACGGTGACCTGGATGGGGACTGGCCAGCGCCGATGCTCCA
  GAAGCCCCCGTACCAGGATGGCCTGAAACCAGAAGGTATTCATAAAGACGTCTGGCTCCACCGTAAGATG
  AAGAATACCGACTATTTGACGCAGTATGACCTCTCAGAATTTGAGAACATTGGTGCCATTGGCCTGGAGC
  TTTGGCAGGTGAGATCTGGAACCATTTTTGATAACTTTCTGATCACAGATGATGAAGAGTATGCAGATAA
  TTTTGGCAAGGCCACCTGGGGCGAAACCAAGGGTCCAGAAAGGGAGATGGATGCCATACAGGCCAAGGAG
  GAAATGAAGAAGGCCCGCGAGGAAGAGGAGGAAGAGCTGCTGTCGGGAAAAATTAACAGGCACGAACATT
  ACTTCAATCAATTTCACAGAAGGAATGAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205398 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARALVQFWAICMLRVALATVYFQEEFLDGEHWRNRWLQSTNDSRFGHFRLSSGKFYGHKEKDKGLQTTQ
  NGRFYAISARFKPFSNKGKTLVIQYTVKHEQKMDCGGGYIKVFPADIDQKNLNGKSQYYIMFGPDICGFD
  IKKVHVILHFKNKYHENKKLIRCKVDGFTHLYTLILRPDLSYDVKIDGQSIESGSIEYDWNLTSLKKETS
  PAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKDVWLHRKM
  KNTDYLTQYDLSEFENIGAIGLELWQVRSGTIFDNFLITDDEEYADNFGKATWGETKGPEREMDAIQAKE
  EMKKAREEEEEELLSGKINRHEHYFNQFHRRNEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145046
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145046.1, NM_145046.2, NM_145046.3, NM_145046.4, NP_659483.1
• RefSeq Size: 1295 bp
• RefSeq ORF: 1155 bp
• Locus ID: 125972
• MW: 45 kDa
• Gene Summary: The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy. [provided by RefSeq, Dec 2011]