PRPF4B (NM_003913) Human Tagged ORF Clone

CAT#: RC205508

PRPF4B (Myc-DDK-tagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)

Product Images

Western blot validation of overexpression lysate (Cat# LY401286) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205508 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRPF4B
• Synonyms: dJ1013A10.1; PR4H; PRP4; PRP4H; PRP4K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205508 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCTGAAAAGAGTA
  TAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCAAAATAAGCACAGTCGTCACAAAAAAAAGAA
  GCATAAACACAGAAGTAAACATAAGAAACATAAACATTCCTCAGAAGAAGACAAGGATAAAAAACATAAA
  CATAAGCATAAACATAAGAAACACAAAAGAAAAGAGGTTATTGATGCTTCTGATAAAGAGGGTATGTCTC
  CAGCAAAAAGAACTAAACTTGATGATTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAA
  GGCCGAACTTGATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTTGCAAGGTTAT
  GAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGGCAAGAAATGGAAATAGGTCTAGTACTAGAT
  CTTCAAGTACAAAGGGGAAACTTGAACTTGTGGACAATAAAATTACTACAAAGAAACGAAGTAAAAGCAG
  ATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAAAGTAAGGGGGGTATTGAAATCGTTAAAGAG
  AAAACAACTAGGAGCAAGTCAAAGGAGAGGAAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAG
  ATCAAGCAAGGAAATCAAAATCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTAAGGCCAGATC
  TCCTACTGATGATAAGGTTAAAATTGAAGATAAAAGTAAATCAAAAGATAGGAAAAAATCCCCAATTATA
  AATGAAAGTAGAAGTCGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGTAAATCCAAAG
  ACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGATAAAGAAAAGAAGCCAATTAA
  ATCTCCCTCTAAAGATGCTTCATCTGGGAAAGAAAATAGGTCACCCAGCAGAAGACCTGGTCGTAGTCCT
  AAAAGAAGAAGTTTGTCTCCAAAACCACGTGATAAATCAAGAAGAAGCAGGTCTCCACTTTTGAATGATA
  GAAGATCTAAGCAGAGCAAATCCCCCTCGCGGACACTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTT
  AGAAAGAAAACGACGAGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATC
  CTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTCTCCAACCCGAAGAAGAAGTA
  GATCTCCCATTAGAAGGAGGTCTCGTTCCCCACTCAGACGTAGCAGGTCTCCAAGAAGAAGAAGCAGATC
  TACTCGGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGCTTGCGAAGGCGGTCTCGATCACGCGGTGGT
  CGTAGACGAAGGAGCAGAAGCAAAGTAAAGGAAGATAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAG
  TTGAGCAGGAATCTTCGTCTGATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCT
  AATAGAACAGAGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAATACCTTGCTGAAGATAGCAAC
  ATGTCTGTGCCATCTGAACCAAGCAGCCCCCAGAGCAGTACGAGAACACGATCACCATCTCCAGATGACA
  TTCTGGAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTTGATACATTTGAGGCCTCAGT
  GAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAATGGTTCATCTCAGAAGAAGTTGTTGGCACCT
  GATATGTTTACAGAATCTGATGATATGTTTGCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCA
  TTGGAAAAGATTTCAAAGAGAATCCCAACCTCAGAGATAACTGGACCGATGCAGAAGGCTATTATCGTGT
  GAACATAGGTGAAGTCCTAGATAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAAT
  GTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAAAGATCATCAGAAACAATGAGC
  TCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAAAAAACTTAATGATGCTGATCCTGATGACAA
  ATTTCATTGTCTGAGACTCTTAAGGCACTTCTATCACAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTC
  AGCATGAACTTACGAGAGGTGTTAAAAAAATATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGAT
  CCTATAGTCAGCAGTTGTTCCTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAA
  GCCAGACAATATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTCGGCTTCACAT
  GTTGCGGATAATGACATAACACCTTATCTTTTCAGTAGATTTTATCGTGCTCCTGAAATCATTATAGGTA
  AAAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATACGAACTCTATACTGGAAAAAT
  TTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTTGCAATGGATCTCAAAGGAAAGATGCCAAAT
  AAGATGATTCGAAAAGGTGTGTTCAAAGATCAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAG
  TTGATAAAGTAACAGAGAGGGAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGGACCTGTTGGC
  TGACTTGATTGGGTGCCAGAGACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTG
  GACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCACGCCTTCATCC
  AGGAAAAAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205508 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAETQSLREQPEMEDANSEKSINEENGEVSEDQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDKDKKHK
  HKHKHKKHKRKEVIDASDKEGMSPAKRTKLDDLALLEDLEKQRALIKAELDNELMEGKVQSGMGLILQGY
  ESGSEEEGEIHEKARNGNRSSTRSSSTKGKLELVDNKITTKKRSKSRSKERTRHRSDKKKSKGGIEIVKE
  KTTRSKSKERKKSKSPSKRSKSQDQARKSKSPTLRRRSQEKIGKARSPTDDKVKIEDKSKSKDRKKSPII
  NESRSRDRGKKSRSPVDLRGKSKDRRSRSKERKSKRSETDKEKKPIKSPSKDASSGKENRSPSRRPGRSP
  KRRSLSPKPRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRLSSPRTRPRDDI
  LSRRERSKDASPINRWSPTRRRSRSPIRRRSRSPLRRSRSPRRRSRSTRRRDRGRRSRSRLRRRSRSRGG
  RRRRSRSKVKEDKFKGSLSEGMKVEQESSSDDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSN
  MSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAP
  DMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSN
  VVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLLRHFYHKQHLCLVFEPL
  SMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASH
  VADNDITPYLFSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPN
  KMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLL
  DQILMLDPAKRISINQALQHAFIQEKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003913
• ORF Size: 3021 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003913.2, NM_003913.3, NM_003913.4, NP_003904.2
• RefSeq Size: 7482 bp
• RefSeq ORF: 3024 bp
• Locus ID: 8899
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 117 kDa
• Gene Summary: Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008]