PRPF4B (NM_003913) Human Untagged Clone

CAT#: SC323624

PRPF4B (untagged)-Kinase deficient mutant (K717M) of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRPF4B
• Synonyms: dJ1013A10.1; PR4H; PRP4; PRP4H; PRP4K
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323624 sequence for NM_003913 edited (data generated by NextGen Sequencing)
  ATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCT
  GAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCAAAATAAGCAC
  AGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACATTCC
  TCAGAAGAAGACAAGGATAAAAAACATAAACATAAGCATAAACATAAGAAACACAAAAGA
  AAAGAGATTATTGATGCTTCTGATAAAGAGGGTATGTCTCCAGCAAAAAGAACTAAACTT
  GATGATTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAAGGCCGAACTT
  GATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTTGCAAGGTTAT
  GAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGGCAAGAAATGGAAATAGGTCT
  AGTACTAGATCTTCAAGTACAAAGGGGAAACTTGAACTTGTGGACAATAAAATTACTACA
  AAGAAACGAAGTAAAAGCAGATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAA
  AGTAAGGGGGGTATTGAAATCGTTAAAGAGAAAACAACTAGGAGCAAGTCAAAGGAGAGG
  AAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAGATCAAGCAAGGAAATCAAAA
  TCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTAAGGCCAGATCTCCTACTGAT
  GATAAGGTTAAAATTGAAGATAAAAGTAAATCAAAAGATAGGAAAAAATCCCCAATTATA
  AATGAAAGTAGAAGTCGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGT
  AAATCCAAAGACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGAT
  AAAGAAAAGAAGCCAATTAAATCTCCCTCTAAAGATGCTTCATCTGGGAAAGAAAATAGG
  TCACCCAGCAGAAGACCTGGTCGTAGTCCTAAAAGAAGAAGTTTGTCTCCAAAACCACGT
  GATAAATCAAGAAGAAGCAGGTCTCCACTTTTGAATGATAGAAGATCTAAGCAGAGCAAA
  TCCCCCTCGCGGACACTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTTAGAAAGAAAA
  CGACGAGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATC
  CTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTCTCCAACCCGA
  AGAAGAAGTAGATCTCCCATTAGAAGGAGGTCTCGTTCCCCACTCAGACGTAGCAGGTCT
  CCAAGAAGAAGAAGCAGATCTCCTCGGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGC
  TTGCGAAGGCGGTCTCGATCACGCGGTGGTCGTAGACGAAGGAGCAGAAGCAAAGTAAAG
  GAAGATAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAGTTGAGCAGGAATCTTCGTCT
  GATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCTAATAGAACAG
  AGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAATACCTTGCTGAAGATAGCAAC
  ATGTCTGTGCCATCTGAACCAAGCAGCCCCCAGAGCAGTACGAGAACACGATCACCATCT
  CCAGATGACATTCTGGAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTT
  GATACATTTGAGGCCTCAGTGAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAAT
  GGTTCATCTCAGAAGAAGTTGTTGGCACCTGATATGTTTACAGAATCTGATGATATGTTT
  GCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCATTGGAAAAGATTTCAAAGAG
  AATCCCAACCTCAGAGATAACTGGACCGATGCAGAAGGCTATTATCGTGTGAACATAGGT
  GAAGTCCTAGATAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAAT
  GTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAATGATCATCAGA
  AACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAAAAAACTTAAT
  GATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTCAGGCACTTCTATCACAAG
  CAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTTACGAGAGGTGTTAAAAAAA
  TATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTC
  CTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAGACAAT
  ATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTCGGCTTCACAT
  GTTGCGGATAATGACATAACACCTTATCTTGTCAGTAGATTTTATCGTGCTCCTGAAATC
  ATTATAGGTAAAAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATAC
  GAACTCTATACTGGAAAAATTTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTT
  GCAATGGATCTCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCAAAGAT
  CAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGATAAAGTAACAGAGAGG
  GAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGGACCTGTTGGCTGACTTGATT
  GGGTGCCAGAGACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTG
  GACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCAC
  GCCTTCATCCAGGAAAAAATTTAA
  
  Clone variation with respect to NM_003913.4
  2150 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003913 unedited
  CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGAGCCGCCGC
  CACCGCCGCCGAGGAGTCAGGAAGTTCAAGATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAG
  AGATGGAAGATGCTAATTCTGAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCA
  AAATAAGCACAGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACGTTCC
  TCAGAGGAGAACAAGGATAAAAAACATAAACATAAGCTTAACCTTAAGAAACCCAAAAGAAAAGGAGATT
  ATTGATGGCTTCCTGATAAGAAGGGTATGGTCTCCGGCAAAAAGACCTAAACTGATTGATTTACCTTGGT
  TGGAGAACTTGGAAAAACGGAGAGCCTGGTTAAAGGCGAACTTGTTAGGAGTTTGGGAGGGAGGGTCCGG
  TTGTGATGGGGGCTATTTTGCAGGTTAGACCGGTTCTGAAGAGGGGGGAATTCGGAAAGGGAAAATGAAA
  TGGTATATATATATCTCGATAAAGGGGACTCTGCTTTGTGCCCTAAAATTCCTCAGAACGAATTAACCGT
  CCCAGACGGCTTCCTTGGTGGATAAGAAGATGGGGGTTGAACGTTAGGAAACTTGGCGTCTAGGAGAAAT
  TAGCTCCAAATGTCCATAGCGGACATCTCTCTAGAGTCAGAATGAGCGATCTCGAAAGGATGAAGATACA
  TAGGATCCTAAGTAGTGCTTCGTAATCTTCTTTG
  
  Kinase domain sequence

  >SC323624 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GKGCWTGGCTAMCTGGGCAGGTGTATTCAGTAATGTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAA
  GAAGTGGCTGTAATGATCATCAGAAACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCT
  TGAAAAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTCAGGCACTTCTATCA
  CAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTT
  
• Restriction Sites: Please inquire
• ACCN: NM_003913
• ORF Size: 3024 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003913.3, NP_003904.2
• RefSeq Size: 6681
• RefSeq ORF: 3024
• Locus ID: 8899
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the protein-coding transcript.