Carbohydrate sulfotransferase 1 (CHST1) (NM_003654) Human Tagged ORF Clone

CAT#: RC205588

CHST1 (Myc-DDK-tagged)-Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418521) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205588 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHST1
• Synonyms: C6ST; GST-1; KS6ST; KSGal6ST; KSST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205588 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATGTTCCTGGAAGGCCGTCCTCCTCCTTGCCCTGGCCTCCATTGCCATCCAGTACACGGCCATCC
  GCACCTTCACCGCCAAGTCCTTTCACACCTGCCCCGGGCTGGCAGAGGCCGGGCTGGCCGAGCGACTGTG
  CGAGGAGAGCCCCACCTTCGCCTACAACCTCTCCCGCAAGACCCACATCCTCATCCTGGCCACCACGCGC
  AGCGGCTCCTCCTTCGTGGGCCAGCTCTTCAACCAGCACCTGGACGTCTTCTACCTGTTTGAGCCCCTCT
  ACCACGTCCAGAACACGCTCATCCCCCGCTTCACCCAGGGCAAGAGCCCGGCCGACCGGCGGGTCATGCT
  AGGCGCCAGCCGCGACCTCCTGCGGAGCCTCTACGACTGCGACCTCTACTTCCTGGAGAACTACATCAAG
  CCGCCGCCGGTCAACCACACCACCGACAGGATCTTCCGCCGCGGGGCCAGCCGGGTCCTCTGCTCCCGGC
  CTGTGTGCGACCCTCCGGGGCCAGCCGACCTGGTCCTGGAGGAGGGGGACTGTGTGCGCAAGTGCGGGCT
  ACTCAACCTGACCGTGGCGGCCGAGGCGTGCCGCGAGCGCAGCCACGTGGCCATCAAGACGGTGCGCGTG
  CCCGAGGTGAACGACCTGCGCGCCCTGGTGGAAGACCCGCGATTAAACCTCAAGGTCATCCAGCTGGTCC
  GAGACCCCCGCGGCATTCTGGCTTCGCGCAGCGAGACCTTCCGCGACACGTACCGGCTCTGGCGGCTCTG
  GTACGGCACCGGGAGGAAACCCTACAACCTGGACGTGACGCAGCTGACCACGGTGTGCGAGGACTTCTCC
  AACTCCGTGTCCACCGGCCTCATGCGGCCCCCGTGGCTCAAGGGCAAGTACATGTTGGTGCGCTACGAGG
  ACCTGGCTCGGAACCCTATGAAGAAGACCGAGGAGATCTACGGGTTCCTGGGCATCCCGCTGGACAGCCA
  CGTGGCCCGCTGGATCCAGAACAACACGCGGGGCGACCCCACCCTGGGCAAGCACAAATACGGCACCGTG
  CGAAACTCGGCGGCCACGGCCGAGAAGTGGCGCTTCCGCCTCTCCTACGACATCGTGGCCTTTGCCCAGA
  ACGCCTGCCAGCAGGTGCTGGCCCAGCTGGGCTACAAGATCGCCGCCTCGGAGGAGGAGCTGAAGAACCC
  CTCGGTCAGCCTGGTGGAGGAGCGGGACTTCCGCCCCTTCTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205588 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAERLCEESPTFAYNLSRKTHILILATTR
  SGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIPRFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIK
  PPPVNHTTDRIFRRGASRVLCSRPVCDPPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRV
  PEVNDLRALVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVCEDFS
  NSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQNNTRGDPTLGKHKYGTV
  RNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIAASEEELKNPSVSLVEERDFRPFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003654
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003654.1, NM_003654.2, NM_003654.3, NM_003654.4, NM_003654.5, NP_003645.1
• RefSeq Size: 3947 bp
• RefSeq ORF: 1236 bp
• Locus ID: 8534
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Keratan sulfate biosynthesis, Metabolic pathways
• MW: 46.7 kDa
• Gene Summary: This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]