ANGEL1 (NM_015305) Human Tagged ORF Clone

CAT#: RC205599

ANGEL1 (Myc-DDK-tagged)-Human angel homolog 1 (Drosophila) (ANGEL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY414639) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205599 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ANGEL1 protein (Cat# TP305599). The protein was produced from HEK293T cells transfected with ANGEL1 cDNA clone (Cat# RC205599) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANGEL1
• Synonyms: Ccr4e; KIAA0759
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205599 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCGTCGTGCTTGTGTTACCTGCTGCTGCCGGCCACGCGCCTCTTCCGCGCCCTCTCAGATGCTT
  TCTTCACATGTCGAAAAAATGTCCTTCTGGCGAACAGCTCATCCCCCCAGGTAGAGGGCGACTTTGCCAT
  GGCCCCTCGGGGCCCTGAGCAGGAGGAATGTGAGGGCCTGCTGCAGCAGTGGCGAGAAGAAGGGTTGAGC
  CAGGTGCTCTCAACTGCAAGTGAGGGGCCCCTTATAGATAAAGGACTAGCCCAGAGCAGCCTGGCACTTC
  TGATGGATAATCCTGGAGAAGAGAATGCTGCTTCAGAGGACAGGTGGTCCAGCAGGCAGCTGAGTGACCT
  TCGGGCTGCAGAGAACCTGGATGAGCCTTTCCCTGAGATGCTAGGAGAGGAGCCACTGCTGGAGGTGGAG
  GGGGTGGAGGGCTCCATGTGGGCAGCTATCCCCATGCAGTCGGAGCCCCAGTATGCAGACTGTGCTGCCC
  TCCCAGTGGGTGCCCTGGCCACAGAGCAGTGGGAAGAGGACCCAGCGGTGTTGGCCTGGAGCATAGCACC
  TGAGCCTGTGCCCCAGGAAGAGGCTTCCATCTGGCCCTTTGAGGGCCTGGGGCAGTTGCAGCCTCCCGCA
  GTGGAAATACCATATCATGAAATTTTGTGGCGAGAATGGGAGGATTTCTCCACCCAGCCAGATGCTCAGG
  GCCTGAAGGCAGGAGATGGCCCTCAGTTCCAGTTCACTCTGATGTCTTATAACATCCTGGCTCAGGACCT
  GATGCAGCAGAGCTCAGAGCTCTATCTACATTGCCATCCAGACATCCTCAATTGGAACTATCGCTTCGTG
  AACCTCATGCAGGAATTCCAGCACTGGGACCCTGATATCCTGTGTCTCCAGGAAGTCCAGGAAGATCATT
  ACTGGGAGCAGCTGGAACCCTCTCTGCGAATGATGGGCTTTACCTGTTTCTACAAGAGGAGGACTGGGTG
  TAAAACCGATGGCTGTGCTGTCTGCTACAAGCCTACCAGATTCCGCCTGCTCTGTGCTAGCCCTGTGGAG
  TACTTCCGGCCTGGCTTGGAGCTACTTAATCGGGATAATGTGGGCTTAGTGTTGCTACTGCAACCACTCG
  TCCCAGAAGGCCTGGGACAAGTCTCGGTGGCCCCGCTGTGTGTGGCAAATACCCATATCCTTTACAACCC
  ACGCCGGGGCGATGTCAAGCTGGCCCAGATGGCCATTCTCCTGGCGGAAGTGGGCAAGGTGGCCAGACTG
  TCAGATGGCAGCCACTGCCCCATCATCTTGTGCGGGGACCTAAATTCTGTCCCTGATTCACCTCTCTACA
  ACTTCATCAGGGATGGAGAGCTCCAGTACCATGGGATGCCAGCCTGGAAGGTATCTGGACAGGAAGACTT
  CTCCCATCAGCTTTACCAGAGGAAGCTGCAGGCCCCACTGTGGCCCAGCTCCCTGGGCATCACTGATTGC
  TGTCAGTATGTCACCTCCTGTCACCCCAAGAGATCAGAGAGACGCAAGTATGGCCGAGACTTCCTGCTAC
  GTTTCCGCTTCTGCAGCATCGCTTGTCAGCGACCAGTAGGACTGGTCCTTATGGAAGGAGTGACAGATAC
  TAAGCCAGAGCGACCTGCGGGTTGGGCTGAGTCTGTCCTTGAGGAAGATGCATCGGAGCTTGAGCCTGCC
  TTCTCCAGGACTGTAGGTACCATCCAGCACTGCCTCCACCTGACGTCAGTATATACCCACTTCCTGCCCC
  AGCGTGGCCGCCCAGAGGTCACTACAATGCCATTGGGTCTTGGAATGACAGTAGATTACATCTTCTTCTC
  AGCTGAGTCCTGTGAGAATGGGAACAGAACTGATCACAGGCTGTATCGAGATGGAACTCTCAAGCTCCTG
  GGTCGTCTCTCCCTTCTCTCTGAAGAGATACTCTGGGCTGCCAATGGCTTACCCAACCCCTTCTGCTCTT
  CAGACCACCTCTGCCTGCTAGCCAGCTTCGGGATGGAAGTCACCGCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205599 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIASCLCYLLLPATRLFRALSDAFFTCRKNVLLANSSSPQVEGDFAMAPRGPEQEECEGLLQQWREEGLS
  QVLSTASEGPLIDKGLAQSSLALLMDNPGEENAASEDRWSSRQLSDLRAAENLDEPFPEMLGEEPLLEVE
  GVEGSMWAAIPMQSEPQYADCAALPVGALATEQWEEDPAVLAWSIAPEPVPQEEASIWPFEGLGQLQPPA
  VEIPYHEILWREWEDFSTQPDAQGLKAGDGPQFQFTLMSYNILAQDLMQQSSELYLHCHPDILNWNYRFV
  NLMQEFQHWDPDILCLQEVQEDHYWEQLEPSLRMMGFTCFYKRRTGCKTDGCAVCYKPTRFRLLCASPVE
  YFRPGLELLNRDNVGLVLLLQPLVPEGLGQVSVAPLCVANTHILYNPRRGDVKLAQMAILLAEVGKVARL
  SDGSHCPIILCGDLNSVPDSPLYNFIRDGELQYHGMPAWKVSGQEDFSHQLYQRKLQAPLWPSSLGITDC
  CQYVTSCHPKRSERRKYGRDFLLRFRFCSIACQRPVGLVLMEGVTDTKPERPAGWAESVLEEDASELEPA
  FSRTVGTIQHCLHLTSVYTHFLPQRGRPEVTTMPLGLGMTVDYIFFSAESCENGNRTDHRLYRDGTLKLL
  GRLSLLSEEILWAANGLPNPFCSSDHLCLLASFGMEVTAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015305
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015305.1, NM_015305.2, NM_015305.3, NP_056120.1
• RefSeq Size: 4131 bp
• RefSeq ORF: 2013 bp
• Locus ID: 23357
• Domains: Exo_endo_phos
• MW: 75.2 kDa