KCTD6 (NM_153331) Human Tagged ORF Clone

CAT#: RC205633

KCTD6 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 6 (KCTD6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426928) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225297 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCTD6 protein (Cat# TP305633). The protein was produced from HEK293T cells transfected with KCTD6 cDNA clone (Cat# RC205633) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD6
• Synonyms: KCASH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205633 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAATGGAGACTGGGGCTATATGATGACTGACCCAGTCACATTAAATGTAGGTGGACACTTGTATA
  CAACGTCTCTCACCACATTGACGCGTTACCCGGATTCCATGCTTGGAGCTATGTTTGGGGGGGACTTCCC
  CACAGCTCGAGACCCTCAAGGCAATTACTTTATTGATCGAGATGGACCTCTTTTCCGATATGTCCTCAAC
  TTCTTAAGAACTTCAGAATTGACCTTACCGTTGGATTTTAAGGAATTTGATCTGCTTCGGAAAGAAGCAG
  ATTTTTACCAGATTGAGCCCTTGATTCAGTGTCTCAATGATCCTAAGCCTTTGTATCCCATGGATACTTT
  TGAAGAAGTTGTGGAGCTGTCTAGTACTCGGAAGCTTTCTAAGTACTCCAACCCAGTGGCTGTCATCATA
  ACGCAACTAACCATCACCACTAAGGTCCATTCCTTACTAGAAGGCATCTCAAATTATTTTACCAAGTGGA
  ATAAGCACATGATGGACACCAGAGACTGCCAGGTTTCCTTTACTTTTGGACCCTGTGATTATCACCAGGA
  AGTTTCTCTTAGGGTCCACCTGATGGAATACATTACAAAACAAGGTTTCACGATCCGCAACACCCGGGTG
  CATCACATGAGTGAGCGGGCCAATGAAAACACAGTGGAGCACAACTGGACTTTCTGTAGGCTAGCCCGGA
  AGACAGACGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205633 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDNGDWGYMMTDPVTLNVGGHLYTTSLTTLTRYPDSMLGAMFGGDFPTARDPQGNYFIDRDGPLFRYVLN
  FLRTSELTLPLDFKEFDLLRKEADFYQIEPLIQCLNDPKPLYPMDTFEEVVELSSTRKLSKYSNPVAVII
  TQLTITTKVHSLLEGISNYFTKWNKHMMDTRDCQVSFTFGPCDYHQEVSLRVHLMEYITKQGFTIRNTRV
  HHMSERANENTVEHNWTFCRLARKTDD
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153331
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153331.1, NM_153331.2, NM_153331.3, NP_699162.2
• RefSeq Size: 1840 bp
• RefSeq ORF: 714 bp
• Locus ID: 200845
• Domains: BTB, K_tetra
• Protein Families: Ion Channels: Other
• MW: 27.6 kDa