VAM1 (MPP6) (NM_016447) Human Tagged ORF Clone

CAT#: RC205667

MPP6 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) (MPP6)

Product Images

Western blot validation of overexpression lysate (Cat# LY402551) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205667 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPP6
• Synonyms: p55T; PALS2; VAM-1; VAM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205667 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAAGTCTTGGAAAACCTTACGGAGCTGCCCTCGTCTACTGGAGCAGAAGAAATAGACCTAATTT
  TCCTCAAGGGAATTATGGAGAATCCTATTGTAAAATCACTTGCTAAGGCTCATGAGAGGCTAGAAGATTC
  CAAACTAGAAGCTGTCAGTGACAATAACTTGGAATTAGTCAATGAAATTCTTGAAGACATCACTCCTCTA
  ATAAATGTGGATGAAAATGTGGCAGAATTGGTTGGTATACTCAAAGAACCTCACTTCCAGTCACTGTTGG
  AGGCCCATGATATTGTGGCATCAAAGTGTTATGATTCACCTCCATCAAGCCCAGAAATGAATAATTCTTC
  TATCAATAATCAGTTATTACCAGTAGATGCCATTCGTATTCTTGGTATTCACAAAAGAGCTGGGGAACCA
  CTGGGTGTGACATTTAGGGTTGAAAATAATGATCTGGTAATTGCCCGAATCCTCCATGGGGGAATGATAG
  ATCGACAAGGTCTACTTCATGTGGGAGATATAATTAAAGAAGTCAATGGCCATGAGGTTGGAAATAATCC
  AAAGGAATTACAAGAATTACTGAAAAATATTAGTGGAAGTGTCACCCTAAAAATCTTACCAAGTTATAGA
  GATACCATTACTCCTCAACAGGTATTTGTGAAGTGTCATTTTGATTATAATCCATACAATGACAACCTAA
  TACCTTGCAAAGAAGCAGGATTGAAGTTTTCCAAAGGAGAAATTCTTCAGATTGTAAATAGAGAAGATCC
  AAATTGGTGGCAGGCTAGCCATGTAAAAGAGGGAGGAAGCGCTGGTCTCATTCCAAGCCAGTTCCTGGAA
  GAGAAGAGAAAGGCATTTGTTAGAAGAGACTGGGACAATTCAGGACCTTTTTGTGGAACTATAAGTAGCA
  AAAAAAAGAAAAAGATGATGTATCTCACAACCAGAAATGCAGAATTTGATCGTCATGAAATCCAGATATA
  TGAGGAGGTAGCCAAAATGCCTCCCTTCCAGAGAAAAACATTAGTATTGATAGGAGCTCAAGGTGTAGGC
  CGAAGAAGCTTGAAAAACAGGTTCATAGTATTGAATCCCACTAGATTTGGAACTACGGTGCCATTTACTT
  CACGGAAACCAAGGGAAGATGAAAAAGATGGCCAGGCATATAAGTTTGTGTCACGATCTGAGATGGAAGC
  AGATATTAAAGCTGGAAAGTATTTGGAACATGGGGAATATGAAGGAAATCTCTATGGAACCAAAATTGAT
  TCTATTCTTGAGGTTGTCCAAACTGGACGGACTTGCATTCTGGATGTCAACCCACAAGCACTGAAAGTAT
  TGAGGACATCAGAGTTTATGCCCTATGTGGTATTTATTGCGGCTCCGGAGCTAGAGACGTTACGTGCCAT
  GCACAAGGCTGTGGTGGATGCAGGAATCACTACCAAGCTTCTGACCGACTCTGACTTGAAGAAAACAGTG
  GATGAAAGTGCACGGATTCAGAGAGCATACAACCACTATTTTGATTTGATCATCATAAATGATAATCTAG
  ACAAAGCCTTTGAAAAACTGCAAACTGCCATAGAGAAACTGAGAATGGAACCACAGTGGGTCCCAATCAG
  CTGGGTTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205667 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQQVLENLTELPSSTGAEEIDLIFLKGIMENPIVKSLAKAHERLEDSKLEAVSDNNLELVNEILEDITPL
  INVDENVAELVGILKEPHFQSLLEAHDIVASKCYDSPPSSPEMNNSSINNQLLPVDAIRILGIHKRAGEP
  LGVTFRVENNDLVIARILHGGMIDRQGLLHVGDIIKEVNGHEVGNNPKELQELLKNISGSVTLKILPSYR
  DTITPQQVFVKCHFDYNPYNDNLIPCKEAGLKFSKGEILQIVNREDPNWWQASHVKEGGSAGLIPSQFLE
  EKRKAFVRRDWDNSGPFCGTISSKKKKKMMYLTTRNAEFDRHEIQIYEEVAKMPPFQRKTLVLIGAQGVG
  RRSLKNRFIVLNPTRFGTTVPFTSRKPREDEKDGQAYKFVSRSEMEADIKAGKYLEHGEYEGNLYGTKID
  SILEVVQTGRTCILDVNPQALKVLRTSEFMPYVVFIAAPELETLRAMHKAVVDAGITTKLLTDSDLKKTV
  DESARIQRAYNHYFDLIIINDNLDKAFEKLQTAIEKLRMEPQWVPISWVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016447
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016447.1, NM_016447.2, NM_016447.3, NP_057531.2
• RefSeq Size: 2201 bp
• RefSeq ORF: 1623 bp
• Locus ID: 51678
• Domains: SH3, PDZ, L27, Guanylate_kin, GuKc
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 61.1 kDa
• Gene Summary: Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily (Tseng et al., 2001 [PubMed 11311936]). [supplied by OMIM, Mar 2008]