PHACS (ACCS) (NM_032592) Human Tagged ORF Clone

CAT#: RC205806

ACCS (Myc-DDK-tagged)-Human 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional) (ACCS), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426720) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225861 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ACCS protein (Cat# TP305806). The protein was produced from HEK293T cells transfected with ACCS cDNA clone (Cat# RC205806) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACCS
• Synonyms: ACS; PHACS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205806 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCCTTCCTCAAAAGGACTTCAGGGCTCCCACCACCTGTCTGGGCCCCACCTGCATGCAGGACC
  TGGGCAGTAGCCATGGGGAAGATCTGGAAGGAGAATGCTCCAGAAAACTGGACCAGAAGCTGCCAGAGCT
  CCGTGGAGTGGGTGATCCTGCCATGATCTCCTCTGATACCTCCTACCTGTCCTCTAGAGGAAGAATGATT
  AAATGGTTCTGGGATTCAGCTGAGGAGGGCTACAGGACCTACCACATGGATGAGTATGATGAGGACAAGA
  ACCCCAGTGGCATCATTAACTTGGGCACCAGTGAGAACAAACTCTGCTTTGACCTGCTGTCCTGGCGGCT
  GAGTCAGCGCGACATGCAGAGGGTGGAGCCATCCCTGCTGCAGTATGCTGACTGGAGGGGACATCTGTTC
  CTCCGGGAGGAAGTGGCCAAGTTCCTGTCTTTCTACTGCAAGAGCCCAGTACCCCTCAGACCAGAGAATG
  TGGTTGTCCTGAATGGTGGTGCCTCGCTCTTCTCTGCTCTGGCCACGGTGCTGTGTGAGGCCGGGGAGGC
  TTTCCTGATCCCCACCCCTTACTATGGCGCTATCACACAGCACGTGTGTCTCTATGGCAACATCCGGCTG
  GCCTATGTCTACCTGGACAGTGAGGTCACTGGGCTAGACACACGCCCCTTCCAGCTCACAGTGGAGAAGC
  TGGAGATGGCCCTGAGAGAAGCTCACTCTGAGGGTGTGAAGGTCAAAGGCCTCATCCTCATCAGCCCCCA
  GAACCCTCTGGGTGATGTATACTCCCCTGAAGAGCTACAGGAGTACCTGGTATTTGCCAAGAGGCACAGG
  CTGCATGTGATTGTGGATGAGGTCTACATGTTGTCCGTGTTTGAGAAGTCTGTTGGGTACCGCAGTGTCC
  TAAGCCTGGAAAGGCTCCCTGACCCCCAGAGGACCCATGTGATGTGGGCAACCAGCAAGGACTTCGGGAT
  GTCTGGGCTCCGCTTTGGCACGCTGTACACAGAAAACCAGGATGTGGCCACTGCCGTGGCTTCCCTCTGC
  CGCTACCACGGCCTCAGTGGCTTGGTCCAGTACCAGATGGCACAGCTGCTCCGGGACCGTGACTGGATCA
  ACCAGGTGTACCTGCCGGAAAACCATGCCCGGCTCAAGGCTGCCCACACCTATGTCTCAGAAGAGCTTAG
  GGCATTGGGGATCCCCTTCTTGAGTCGTGGGGCTGGCTTCTTCATCTGGGTTGACTTGAGAAAGTACCTG
  CTCAAGGGCACCTTTGAGGAGGAAATGCTGCTCTGGCGCCGCTTTTTGGACAACAAGGTGCTGCTGTCCT
  TTGGCAAGGCCTTCGAGTGTAAAGAGCCTGGTTGGTTTCGCTTTGTCTTCTCAGACCAGGTCCACCGGCT
  TTGCCTGGGGATGCAGAGGGTCCAGCAGGTGCTTGCAGGCAAATCCCAAGTGGCAGAAGACCCCCGTCCC
  TCTCAGAGCCAGGAGCCAAGTGACCAACGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205806 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFTLPQKDFRAPTTCLGPTCMQDLGSSHGEDLEGECSRKLDQKLPELRGVGDPAMISSDTSYLSSRGRMI
  KWFWDSAEEGYRTYHMDEYDEDKNPSGIINLGTSENKLCFDLLSWRLSQRDMQRVEPSLLQYADWRGHLF
  LREEVAKFLSFYCKSPVPLRPENVVVLNGGASLFSALATVLCEAGEAFLIPTPYYGAITQHVCLYGNIRL
  AYVYLDSEVTGLDTRPFQLTVEKLEMALREAHSEGVKVKGLILISPQNPLGDVYSPEELQEYLVFAKRHR
  LHVIVDEVYMLSVFEKSVGYRSVLSLERLPDPQRTHVMWATSKDFGMSGLRFGTLYTENQDVATAVASLC
  RYHGLSGLVQYQMAQLLRDRDWINQVYLPENHARLKAAHTYVSEELRALGIPFLSRGAGFFIWVDLRKYL
  LKGTFEEEMLLWRRFLDNKVLLSFGKAFECKEPGWFRFVFSDQVHRLCLGMQRVQQVLAGKSQVAEDPRP
  SQSQEPSDQRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032592
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032592.1, NM_032592.2, NM_032592.3, NP_115981.1
• RefSeq Size: 2196 bp
• RefSeq ORF: 1506 bp
• Locus ID: 84680
• Domains: aminotran_1_2
• MW: 57.3 kDa