MAF1 (NM_032272) Human Tagged ORF Clone
CAT#: RC205808
MAF1 (Myc-DDK-tagged)-Human MAF1 homolog (S. cerevisiae) (MAF1)
"NM_032272" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MAF1 |
Synonyms | MGC20332; MGC31779; MGC39758 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205808 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTATTGGAGAACTCGAGCTTTGAAGCCATCAACTCACAGCTGACTGTGGAGACCGGAGATGCCC ACATCATTGGCAGGATTGAGAGCTACTCATGTAAGATGGCAGGAGACGACAAACACATGTTCAAGCAGTT CTGCCAGGAGGGCCAGCCCCACGTGCTGGAGGCACTTTCTCCACCCCAGACTTCAGGACTGAGCCCCAGC AGACTCAGCAAAAGCCAAGGCGGTGAGGAGGAGGGCCCCCTCAGTGACAAGTGCAGCCGCAAGACCCTCT TCTACCTGATTGCCACGCTCAATGAGTCCTTCAGGCCTGACTATGACTTCAGCACAGCCCGCAGCCATGA GTTCAGCCGGGAGCCCAGCCTTAGCTGGGTGGTGAATGCAGTCAACTGCAGTCTGTTCTCAGCTGTGCGG GAGGACTTCAAGGATCTGAAACCACAGCTGTGGAACGCGGTGGACGAGGAGATCTGCCTGGCTGAATGTG ACATCTACAGCTATAACCCAGACTTGGACTCAGATCCCTTCGGGGAGGATGGTAGCCTCTGGTCCTTCAA CTACTTCTTCTACAACAAGCGGCTCAAGCGAATCGTCTTCTTTAGCTGCCGTTCCATCAGTGGCTCCACC TACACACCCTCAGAGGCAGGCAACGAGCTGGACATGGAGCTGGGGGAGGAGGAGGTGGAGGAAGAAAGCA GAAGCAGGGGCAGTGGGGCCGAGGAGACCAGCACCATGGAGGAGGACAGGGTCCCAGTGATCTGTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205808 protein sequence
Red=Cloning site Green=Tags(s) MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPS RLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVR EDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGST YTPSEAGNELDMELGEEEVEEESRSRGSGAEETSTMEEDRVPVICI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032272 |
ORF Size | 768 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032272.1, NM_032272.2, NM_032272.3, NM_032272.4, NP_115648.1, NP_115648.2 |
RefSeq Size | 1828 bp |
RefSeq ORF | 771 bp |
Locus ID | 84232 |
Protein Families | Transcription Factors |
MW | 28.9 kDa |
Gene Summary | This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC105803 | MAF1 (untagged)-Human MAF1 homolog (S. cerevisiae) (MAF1) |
USD 310.00 |
|
RG205808 | MAF1 (GFP-tagged) - Human MAF1 homolog (S. cerevisiae) (MAF1) |
USD 460.00 |
|
RC205808L1 | Lenti ORF clone of Human MAF1 homolog (S. cerevisiae) (MAF1), Myc-DDK-tagged |
USD 768.00 |
|
RC205808L2 | Lenti ORF clone of Human MAF1 homolog (S. cerevisiae) (MAF1), mGFP tagged |
USD 620.00 |
|
RC205808L3 | Lenti ORF clone of Human MAF1 homolog (S. cerevisiae) (MAF1), Myc-DDK-tagged |
USD 620.00 |
|
RC205808L4 | Lenti ORF clone of Human MAF1 homolog (S. cerevisiae) (MAF1), mGFP tagged |
USD 768.00 |
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