CYB5R1 (NM_016243) Human Tagged ORF Clone

CAT#: RC205833

CYB5R1 (Myc-DDK-tagged)-Human cytochrome b5 reductase 1 (CYB5R1)

Product Images

Western blot validation of overexpression lysate (Cat# LY402525) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205833 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CYB5R1 protein (Cat# TP305833). The protein was produced from HEK293T cells transfected with CYB5R1 cDNA clone (Cat# RC205833) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYB5R1
• Synonyms: B5R.1; B5R1; B5R2; humb5R2; NQO3A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205833 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATCCAGACGAGCCCCGTCCTGCTGGCCTCCCTGGGGGTGGGGCTGGTCACTCTGCTCGGCCTGG
  CTGTGGGCTCCTACTTGGTTCGGAGGTCCCGCCGGCCTCAGGTCACTCTCCTGGACCCCAATGAAAAGTA
  CCTGCTACGACTGCTAGACAAGACGACTGTGAGCCACAACACCAAGAGGTTCCGCTTTGCCCTGCCCACC
  GCCCACCACACTCTGGGGCTGCCTGTGGGCAAACATATCTACCTCTCCACCCGAATTGATGGCAGCCTGG
  TCATCAGGCCATACACTCCTGTCACCAGTGATGAGGATCAAGGCTATGTGGATCTTGTCATCAAGGTCTA
  CCTGAAGGGTGTGCACCCCAAATTTCCTGAGGGAGGGAAGATGTCTCAGTACCTGGATAGCCTGAAGGTT
  GGGGATGTGGTGGAGTTTCGGGGGCCAAGCGGGTTGCTCACTTACACTGGAAAAGGGCATTTTAACATTC
  AGCCCAACAAGAAATCTCCACCAGAACCCCGAGTGGCGAAGAAACTGGGAATGATTGCCGGCGGGACAGG
  AATCACCCCAATGCTACAGCTGATCCGGGCCATCCTGAAAGTCCCTGAAGATCCAACCCAGTGCTTTCTG
  CTTTTTGCCAACCAGACAGAAAAGGATATCATCTTGCGGGAGGACTTAGAGGAACTGCAGGCCCGCTATC
  CCAATCGCTTTAAGCTCTGGTTCACTCTGGATCATCCCCCAAAAGATTGGGCCTACAGCAAGGGCTTTGT
  GACTGCCGACATGATCCGGGAACACCTGCCCGCTCCAGGGGATGATGTGCTGGTACTGCTTTGTGGGCCA
  CCCCCAATGGTGCAGCTGGCCTGCCATCCCAACTTGGACAAACTGGGCTACTCACAAAAGATGCGATTCA
  CCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205833 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGIQTSPVLLASLGVGLVTLLGLAVGSYLVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPT
  AHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKV
  GDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFL
  LFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGP
  PPMVQLACHPNLDKLGYSQKMRFTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016243
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016243.1, NM_016243.2, NP_057327.2
• RefSeq Size: 1675 bp
• RefSeq ORF: 918 bp
• Locus ID: 51706
• Domains: NAD_binding_1, FAD_binding_6
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• MW: 34.1 kDa
• Gene Summary: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. [UniProtKB/Swiss-Prot Function]