LSM7 (NM_016199) Human Tagged ORF Clone
CAT#: RC205835
LSM7 (Myc-DDK-tagged)-Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7)
"NM_016199" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LSM7 |
Synonyms | YNL147W |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205835 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATAAGGAGAAGAAGAAAAAGGAGAGCATCTTGGACTTGTCCAAGTACATCGACAAGACGATCC GGGTAAAGTTCCAGGGAGGCCGCGAAGCCAGTGGAATCCTGAAGGGCTTCGACCCACTCCTCAACCTTGT GCTGGACGGCACCATTGAGTACATGCGAGACCCTGACGACCAGTACAAGCTCACGGAGGACACCCGGCAG CTGGGCCTCGTGGTGTGCCGGGGCACGTCCGTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCC CCAACCCCTTCATCCAGCAGCAGGACGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205835 protein sequence
Red=Cloning site Green=Tags(s) MADKEKKKKESILDLSKYIDKTIRVKFQGGREASGILKGFDPLLNLVLDGTIEYMRDPDDQYKLTEDTRQ LGLVVCRGTSVVLICPQDGMEAIPNPFIQQQDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016199 |
ORF Size | 309 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016199.1, NM_016199.2, NP_057283.1 |
RefSeq Size | 536 bp |
RefSeq ORF | 312 bp |
Locus ID | 51690 |
Domains | Sm |
Protein Families | Stem cell - Pluripotency |
Protein Pathways | RNA degradation, Spliceosome |
MW | 11.6 kDa |
Gene Summary | Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. [supplied by OMIM, Apr 2004] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108743 | LSM7 (untagged)-Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7) |
USD 310.00 |
|
RG205835 | LSM7 (GFP-tagged) - Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7) |
USD 460.00 |
|
RC205835L3 | Lenti ORF clone of Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7), Myc-DDK-tagged |
USD 620.00 |
|
RC205835L4 | Lenti ORF clone of Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review