LSM7 (NM_016199) Human Tagged ORF Clone

CAT#: RG205835

LSM7 (GFP-tagged) - Human LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM7)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LSM7
• Synonyms: YNL147W
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205835 representing NM_016199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATAAGGAGAAGAAGAAAAAGGAGAGCATCTTGGACTTGTCCAAGTACATCGACAAGACGATCC
  GGGTAAAGTTCCAGGGAGGCCGCGAAGCCAGTGGAATCCTGAAGGGCTTCGACCCACTCCTCAACCTTGT
  GCTGGACGGCACCATTGAGTACATGCGAGACCCTGACGACCAGTACAAGCTCACGGAGGACACCCGGCAG
  CTGGGCCTCGTGGTGTGCCGGGGCACGTCCGTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCC
  CCAACCCCTTCATCCAGCAGCAGGACGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205835 representing NM_016199
  Red=Cloning site Green=Tags(s)
  
  MADKEKKKKESILDLSKYIDKTIRVKFQGGREASGILKGFDPLLNLVLDGTIEYMRDPDDQYKLTEDTRQ
  LGLVVCRGTSVVLICPQDGMEAIPNPFIQQQDA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016199
• ORF Size: 309 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016199.1, NP_057283.1
• RefSeq Size: 489
• RefSeq ORF: 312
• Locus ID: 51690
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Spliceosome
• Gene Summary: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. [supplied by OMIM, Apr 2004]