GDF3 (NM_020634) Human Tagged ORF Clone

CAT#: RC205901

GDF3 (Myc-DDK-tagged)-Human growth differentiation factor 3 (GDF3)

Product Images

Western blot validation of overexpression lysate (Cat# LY402794) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205901 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GDF3
• Synonyms: KFS3; MCOP7; MCOPCB6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCGTTTCTTGCCAGATTTGGCTTTCAGCTTCCTGTTAATTCTGGCTTTGGGCCAGGCAGTCCAAT
  TTCAAGAATATGTCTTTCTCCAATTTCTGGGCTTAGATAAGGCGCCTTCACCCCAGAAGTTCCAACCTGT
  GCCTTATATCTTGAAGAAAATTTTCCAGGATCGCGAGGCAGCAGCGACCACTGGGGTCTCCCGAGACTTA
  TGCTACGTAAAGGAGCTGGGCGTCCGCGGGAATGTACTTCGCTTTCTCCCAGACCAAGGTTTCTTTCTTT
  ACCCAAAGAAAATTTCCCAAGCTTCCTCCTGCCTGCAGAAGCTCCTCTACTTTAACCTGTCTGCCATCAA
  AGAAAGGGAACAGTTGACATTGGCCCAGCTGGGCCTGGACTTGGGGCCCAATTCTTACTATAACCTGGGA
  CCAGAGCTGGAACTGGCTCTGTTCCTGGTTCAGGAGCCTCATGTGTGGGGCCAGACCACCCCTAAGCCAG
  GTAAAATGTTTGTGTTGCGGTCAGTCCCATGGCCACAAGGTGCTGTTCACTTCAACCTGCTGGATGTAGC
  TAAGGATTGGAATGACAACCCCCGGAAAAATTTCGGGTTATTCCTGGAGATACTGGTCAAAGAAGATAGA
  GACTCAAGGGTGAATTTTCAGCCTGAAGACACCTGTGCCAGACTAAGATGCTCCCTTCATGCTTCCCTGC
  TGGTGGTGACTCTCAACCCTGATCAGTGCCACCCTTCTCGGAAAAGGAGAGCAGCCATCCCTGTCCCCAA
  GCTTTCTTGTAAGAACCTCTGCCACCGTCACCAGCTATTCATTAACTTCCGGGACCTGGGTTGGCACAAG
  TGGATCATTGCCCCCAAGGGGTTCATGGCAAATTACTGCCATGGAGAGTGTCCCTTCTCACTGACCATCT
  CTCTCAACAGCTCCAATTATGCTTTCATGCAAGCCCTGATGCATGCCGTTGACCCAGAGATCCCCCAGGC
  TGTGTGTATCCCCACCAAGCTGTCTCCCATTTCCATGCTCTACCAGGACAATAATGACAATGTCATTCTA
  CGACATTATGAAGACATGGTAGTCGATGAATGTGGGTGTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLRFLPDLAFSFLLILALGQAVQFQEYVFLQFLGLDKAPSPQKFQPVPYILKKIFQDREAAATTGVSRDL
  CYVKELGVRGNVLRFLPDQGFFLYPKKISQASSCLQKLLYFNLSAIKEREQLTLAQLGLDLGPNSYYNLG
  PELELALFLVQEPHVWGQTTPKPGKMFVLRSVPWPQGAVHFNLLDVAKDWNDNPRKNFGLFLEILVKEDR
  DSRVNFQPEDTCARLRCSLHASLLVVTLNPDQCHPSRKRRAAIPVPKLSCKNLCHRHQLFINFRDLGWHK
  WIIAPKGFMANYCHGECPFSLTISLNSSNYAFMQALMHAVDPEIPQAVCIPTKLSPISMLYQDNNDNVIL
  RHYEDMVVDECGCG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020634
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020634.1, NM_020634.2, NP_065685.1
• RefSeq Size: 1224 bp
• RefSeq ORF: 1095 bp
• Locus ID: 9573
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
• MW: 41.5 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein plays a role ocular and skeletal development. Mutations in this gene are associated with microphthalmia, coloboma, and skeletal abnormalities in human patients. [provided by RefSeq, Aug 2016]