EXOSC1 (NM_016046) Human Tagged ORF Clone
CAT#: RC206007
EXOSC1 (Myc-DDK-tagged)-Human exosome component 1 (EXOSC1)
"NM_016046" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | EXOSC1 |
Synonyms | CGI-108; CSL4; Csl4p; p13; SKI4; Ski4p |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206007 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCACCTGTGAGATACTGCATCCCCGGCGAACGTCTGTGTAACTTGGAGGAGGGCAGCCCGGGCA GCGGCACCTACACCCGCCACGGCTACATCTTTTCGTCGCTTGCCGGCTGTCTGATGAAGAGCAGCGAGAA TGGCGCGCTTCCAGTGGTGTCTGTAGTGAGAGAAACAGAGTCCCAGTTACTGCCAGATGTGGGAGCTATT GTAACCTGTAAGGTCTCTAGCATCAATTCACGCTTTGCCAAAGTACACATCCTGTATGTGGGGTCCATGC CTCTTAAGAACTCTTTTCGAGGAACTATCCGCAAGGAAGATGTCCGAGCAACTGAAAAAGACAAGGTTGA AATTTATAAGAGTTTCCGCCCAGGTGACATTGTCTTGGCCAAAGTGATCTCCTTAGGTGATGCACAGTCC AACTACCTGCTAACCACCGCCGAGAACGAGCTGGGAGTGGTGGTAGCCCACAGTGAGTCAGGTATCCAGA TGGTTCCCATCAGCTGGTGTGAGATGCAGTGCCCTAAGACCCACACTAAAGAATTCCGGAAAGTAGCCCG AGTACAACCCGAATTCTTGCAGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206007 protein sequence
Red=Cloning site Green=Tags(s) MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAI VTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQS NYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016046 |
ORF Size | 585 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016046.1, NM_016046.2, NM_016046.3, NM_016046.4, NP_057130.1 |
RefSeq Size | 1150 bp |
RefSeq ORF | 588 bp |
Locus ID | 51013 |
Protein Pathways | RNA degradation |
MW | 21.5 kDa |
Gene Summary | This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114517 | EXOSC1 (untagged)-Human exosome component 1 (EXOSC1) |
USD 420.00 |
|
SC322121 | EXOSC1 (untagged)-Human exosome component 1 (EXOSC1) |
USD 420.00 |
|
RG206007 | EXOSC1 (GFP-tagged) - Human exosome component 1 (EXOSC1) |
USD 460.00 |
|
RC206007L1 | Lenti ORF clone of Human exosome component 1 (EXOSC1), Myc-DDK-tagged |
USD 768.00 |
|
RC206007L2 | Lenti ORF clone of Human exosome component 1 (EXOSC1), mGFP tagged |
USD 620.00 |
|
RC206007L3 | Lenti ORF clone of Human exosome component 1 (EXOSC1), Myc-DDK-tagged |
USD 620.00 |
|
RC206007L4 | Lenti ORF clone of Human exosome component 1 (EXOSC1), mGFP tagged |
USD 620.00 |
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