Cyclophilin 40 (PPID) (NM_005038) Human Tagged ORF Clone

CAT#: RC206039

PPID (Myc-DDK-tagged)-Human peptidylprolyl isomerase D (PPID)

Product Images

Western blot validation of overexpression lysate (Cat# LY401561) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206039 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPID protein (Cat# TP306039). The protein was produced from HEK293T cells transfected with PPID cDNA clone (Cat# RC206039) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPID
• Synonyms: CYP-40; CYPD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206039 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCACCCGTCCCCCCAAGCCAAGCCCTCCAACCCCAGTAACCCTCGAGTCTTCTTTGACGTGGACA
  TCGGAGGGGAGCGAGTTGGTCGAATTGTCTTAGAATTGTTTGCAGATATCGTACCCAAAACTGCGGAAAA
  TTTTCGTGCACTGTGTACAGGAGAAAAAGGCATTGGACACACGACTGGGAAACCTCTCCATTTCAAAGGA
  TGCCCTTTTCATCGAATTATTAAGAAATTTATGATTCAGGGTGGAGACTTCTCAAATCAGAATGGGACAG
  GTGGAGAAAGTATTTATGGTGAAAAATTTGAAGATGAAAATTTCCATTACAAGCATGATCGGGAGGGTTT
  ACTGAGCATGGCAAATGCAGGCCGCAACACAAACGGTTCTCAGTTTTTTATCACAACAGTTCCAACTCCT
  CATTTGGATGGGAAACATGTGGTGTTTGGCCAAGTAATTAAAGGAATAGGAGTGGCAAGGATATTGGAAA
  ATGTGGAAGTGAAAGGTGAAAAACCTGCTAAATTGTGCGTTATTGCAGAATGTGGAGAATTGAAGGAAGG
  AGATGACGGGGGAATATTCCCAAAAGATGGCTCTGGCGACAGTCATCCAGATTTCCCTGAGGATGCGGAT
  ATAGATTTAAAAGATGTAGATAAAATTTTATTAATAACAGAAGACTTAAAAAACATTGGAAATACTTTTT
  TCAAATCCCAGAACTGGGAGATGGCTATTAAAAAATATGCAGAAGTTTTAAGATACGTGGACAGTTCAAA
  GGCTGTTATTGAGACAGCAGATAGAGCCAAGCTGCAACCTATAGCTTTAAGCTGTGTACTGAATATTGGT
  GCTTGTAAACTGAAGATGTCAAATTGGCAGGGAGCAATTGACAGTTGTTTAGAGGCTCTTGAACTAGACC
  CATCAAATACCAAAGCATTGTACCGCAGAGCTCAAGGATGGCAAGGATTAAAAGAATATGATCAAGCATT
  GGCTGATCTTAAGAAAGCTCAGGGGATAGCACCAGAAGATAAAGCTATCCAGGCAGAATTGCTGAAAGTC
  AAACAAAAGATAAAGGCACAGAAAGATAAAGAGAAGGCAGTATATGCAAAAATGTTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206039 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKG
  CPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTP
  HLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDAD
  IDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIG
  ACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKV
  KQKIKAQKDKEKAVYAKMFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005038
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005038.1, NM_005038.2, NP_005029.1
• RefSeq Size: 1851 bp
• RefSeq ORF: 1113 bp
• Locus ID: 5481
• Cytogenetics: 4q32.1
• Domains: TPR, pro_isomerase
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Calcium signaling pathway, Huntington's disease, Parkinson's disease
• MW: 40.8 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]'