PFKFB3 (NM_004566) Human Tagged ORF Clone
CAT#: RC206043L4
- LentiORF®
Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mGFP tagged
"NM_004566" in other vectors (6)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | PFKFB3 |
Synonyms | iPFK-2; IPFK2; PFK2 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC206043).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_004566 |
ORF Size | 1560 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004566.2, NP_004557.1 |
RefSeq Size | 4553 bp |
RefSeq ORF | 1563 bp |
Locus ID | 5209 |
Cytogenetics | 10p15.1 |
Domains | PGAM, 6PF2K |
Protein Families | Druggable Genome |
Protein Pathways | Fructose and mannose metabolism |
MW | 59.6 kDa |
Gene Summary | 'The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117283 | PFKFB3 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1 |
USD 310.00 |
|
RC206043 | PFKFB3 (Myc-DDK-tagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1 |
USD 480.00 |
|
RG206043 | PFKFB3 (GFP-tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1 |
USD 530.00 |
|
RC206043L1 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, Myc-DDK-tagged |
USD 840.00 |
|
RC206043L2 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mGFP tagged |
USD 680.00 |
|
RC206043L3 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, Myc-DDK-tagged |
USD 680.00 |
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