PFKFB3 (NM_004566) Human Untagged Clone

CAT#: SC117283

PFKFB3 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PFKFB3
• Synonyms: iPFK-2; IPFK2; PFK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117283 sequence for NM_004566 edited (data generated by NextGen Sequencing)
  ATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGTGCCCGTGGACCACAGG
  CCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGACCAACTCCCCCACCGTCATCGTCATG
  GTGGGCCTCCCCGCCCGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGCTACCTCAAC
  TGGATTGGCGTCCCCACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAG
  CAGTACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTCCGGAAG
  CAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGCGAAAGAAGGGGGACAA
  ATTGCGGTTTTCGATGCCACCAATACTACTAGAGAGAGGAGACACATGATCCTTCATTTT
  GCCAAAGAAAATGACTTTAAGGCGTTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTT
  GTGGCCTCCAATATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCG
  GCAGAAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTACCAGCCC
  CTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGTGATTGACGTGGGCCGG
  AGGTTCCTGGTGAACCGGGTGCAGGACCACATCCAGAGCCGCATCGTGTACTACCTGATG
  AACATCCACGTGCAGCCGCGTACCATCTACCTGTGCCGGCACGGCGAGAACGAGCACAAC
  CTCCAGGGCCGCATCGGGGGCGACTCAGGCCTGTCCAGCCGGGGCAAGAAGTTTGCCAGT
  GCTCTGAGCAAGTTCGTGGAGGAGCAGAACCTGAAGGACCTGCGCGTGTGGACCAGCCAG
  CTGAAGAGCACCATCCAGACGGCCGAGGCGCTGCGGCTGCCCTACGAGCAGTGGAAGGCG
  CTCAATGAGATCGACGCGGGCGTCTGTGAGGAGCTGACCTACGAGGAGATCAGGGACACC
  TACCCTGAGGAGTATGCGCTGCGGGAGCAGGACAAGTACTATTACCGCTACCCCACCGGG
  GAGTCCTACCAGGACCTGGTCCAGCGCTTGGAGCCAGTGATCATGGAGCTGGAGCGGCAG
  GAGAATGTGCTGGTCATCTGCCACCAGGCCGTCCTGCGCTGCCTGCTTGCCTACTTCCTG
  GATAAGAGTGCAGAGGAGATGCCCTACCTGAAATGCCCTCTTCACACCGTCCTGAAACTG
  ACGCCTGTCGCTTATGGCTGCCGTGTGGAATCCATCTACCTGAACGTGGAGTCCGTCTGC
  ACACACCGGGAGAGGTCAGAGGATGCAAAGAAGGGACCTAACCCGCTCATGAGACGCAAT
  AGTGTCACCCCGCTAGCCAGCCCCGAACCCACCAAAAAGCCTCGCATCAACAGCTTTGAG
  GAGCATGTGGCCTCCACCTCGGCCGCCCTGCCCAGCTGCCTGCCCCCGGAGGTGCCCACG
  CAGCTGCCTGGACAAAACATGAAAGGCTCCCGGAGCAGCGCTGACTCCTCCAGGAAACAC
  TGA
  
  Clone variation with respect to NM_004566.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004566 unedited
  NAAGGGTCAGATATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACCAGCCGC
  TCTCCTGCCAGCGTCGGGATCTCGGCCCCGGGAGGCGGGCCGTCGGGCGCAGCCGCGAAG
  ATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGTGCCCGTGGACCACAGG
  CCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGACCAACTCCCCCACCGTCATCGTCATG
  GTGGGCCTCCCCGCCCGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGCTACCTCAAC
  TGGATTGGCGTCCCCACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAG
  CAGTACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTCCGGAAG
  CAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGCGAAAGAAGGGGGACAA
  ATTGCGGTTTTCGATGCCACCAATACTACTAGAGAGAGGAGACACATGATCCTTCATTTT
  GCCAAAGAAAATGACTTTAAGGCGTTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTT
  GTGGCCTCCAATATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCG
  GCAGAAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTACCAGCCC
  CTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGTGATTGACGTGGGCCGG
  AGGCTCCTGGTGAACCGGGTGCAGGACCACATCCAGAGCCGCATCGTGTACTACCTGATG
  AAATNCAGTGCAGCCGCGTCCATCTACCTGTGCCGGCACGGCGAGAACGAGCACACCTTC
  CAGGCCGATCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004566 unedited
  NNNNNNNTTTGGTACATCTAAGNACCGCGGNCCGCAANCTAGNGATCGGTTTTTTTTTTT
  TTTTTTTTTTGTATTTCAACCAGGTATTTTATTGGTCTGGCAACTGCAAAATATACAAAT
  TTCTGAAAGGCATCTCCTGTTTGAAAGCTAGCATAGCTTTATATCCCAGGTCATTAATTC
  AGACAAATACACAGAACACAGAGAAGTCATAAGTCACACATAGGAGGCTCCCAAACCAAT
  TAACAATGAAACCCAATCTCTACAGCTTTCTATTGTTTAGGCTCCTTATACATATTAAAT
  AAATAAATAGCACATCTGCTATCAAAATAATAAAAAAATAAATTTCAAGTATTACAAAGA
  AAAATATCATTGGTGTGGTTCCAAACAGTTTTTCTTCTCTCTCAAAATGAAGAGCTTTGC
  CCGTGGTCTCAGGACAGAATAAGGAGGGGGGAAACTCACCCAAAAACAAATAGGCAAAGT
  TAAAAATATTGGTCCCCGTTATTTTGTGGGGTGGAGAGTTGGAGGAATCAGTAGACGCCA
  AAAGTTCATGACTGTAGCTCAAACCCAACCCCCGCCCCCGGCCCCCGTCCAATGTCCCCT
  CAAAAGGCAGGTCACTGGCTTGCAAAGTAGGCAGTGTTCTCCTTTCTATAGTATCTCCCT
  CCCTGTCCAGATTCATGTTACCCTCTCTCCTATCTAGGCCCTTCGCAAACTAAAAGTCCC
  GTGTTAGACCAAGGGCTTCTGTATTTAGGTATTGATTTTAGGTAGTCAGAGGGGGAGAGG
  GACCACAGCTATCAAGCCCCTATCTGTATCAAACTTCAGAGTTGATGTTCAAAGAATGAN
  GCCTGCGAGGGGGACACGAGNAACTATTCTCAAGAGCAAGCACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004566
• Insert Size: 4260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004566.2, NP_004557.1
• RefSeq Size: 4497 bp
• RefSeq ORF: 1563 bp
• Locus ID: 5209
• Cytogenetics: 10p15.1
• Domains: PGAM, 6PF2K
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Gene Summary: 'The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]'
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.