SMG5 (NM_015327) Human Tagged ORF Clone

CAT#: RC206137

SMG5 (Myc-DDK-tagged)-Human Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMG5
• Synonyms: EST1B; LPTS-RP1; LPTSRP1; SMG-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206137 representing NM_015327
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAAGGCCCCCCCACAGGGGAGAGCAGCGAGCCCGAAGCAAAAGTCCTCCACACTAAGCGGCTTT
  ACCGGGCTGTGGTGGAGGCTGTGCATCGACTTGACCTCATCCTTTGCAACAAAACTGCTTATCAAGAAGT
  ATTCAAACCAGAAAACATTAGCCTGAGGAACAAGCTGCGTGAGCTCTGCGTCAAGCTTATGTTCCTGCAC
  CCAGTGGACTATGGGAGAAAGGCTGAGGAGCTGCTGTGGAGAAAGGTATACTATGAAGTTATCCAGCTTA
  TCAAGACTAACAAAAAGCACATCCACAGCCGGAGCACTTTGGAATGTGCCTACAGGACGCACCTGGTTGC
  TGGTATTGGCTTCTACCAGCATCTCCTTCTCTATATCCAGTCCCACTACCAGCTGGAACTGCAGTGCTGC
  ATCGACTGGACCCATGTCACTGACCCCCTCATAGGATGCAAGAAGCCAGTGTCTGCCTCAGGGAAGGAGA
  TGGATTGGGCACAGATGGCATGTCACCGATGTCTGGTGTATCTGGGGGATTTGTCCCGATATCAGAATGA
  ATTAGCTGGCGTAGATACCGAGCTGCTAGCCGAGAGATTTTACTACCAAGCCCTGTCAGTAGCTCCTCAG
  ATTGGAATGCCCTTCAATCAGCTGGGCACCCTGGCAGGCAGCAAGTACTATAATGTGGAAGCCATGTATT
  GCTACCTGCGCTGCATCCAGTCAGAAGTGTCCTTTGAGGGAGCCTATGGGAACCTCAAGCGGCTGTATGA
  CAAGGCAGCCAAAATGTACCACCAACTGAAGAAGTGTGAGACTCGGAAACTGTCTCCTGGCAAAAAGCGA
  TGTAAAGACATTAAAAGGTTGCTAGTGAACTTTATGTATCTGCAAAGCCTCCTACAGCCCAAAAGCAGCT
  CCGTGGACTCAGAGCTGACCTCACTTTGCCAGTCAGTCCTGGAGGACTTCAACCTCTGCCTCTTCTACCT
  GCCCTCCTCACCCAACCTCAGCCTGGCCAGTGAGGATGAGGAGGAGTATGAGAGTGGATATGCTTTCCTC
  CCGGACCTTCTCATCTTTCAAATGGTCATCATCTGCCTTATGTGTGTGCACAGCTTGGAGAGAGCAGGAT
  CCAAGCAGTACAGTGCAGCCATTGCCTTCACCCTGGCCCTCTTTTCCCACCTCGTCAATCATGTCAACAT
  ACGGCTGCAGGCTGAGCTGGAAGAGGGCGAGAATCCCGTCCCGGCATTCCAGAGTGATGGCACAGATGAA
  CCAGAGTCCAAGGAACCTGTGGAGAAAGAGGAGGAGCCAGATCCTGAGCCTCCTCCTGTAACACCCCAAG
  TGGGTGAGGGCAGAAAGAGCCGTAAGTTCTCTCGCCTCTCCTGTCTCCGCCGTCGCCGCCACCCACCCAA
  AGTTGGTGATGACAGTGACCTGAGTGAAGGCTTTGAATCGGACTCAAGCCATGACTCAGCCCGGGCCAGT
  GAGGGCTCAGACAGTGGCTCTGACAAGAGTCTTGAAGGTGGGGGAACGGCCTTTGATGCTGAAACAGACT
  CGGAAATGAATAGCCAGGAGTCCCGATCAGACTTGGAAGATATGGAGGAAGAGGAGGGGACACGGTCACC
  AACCCTGGAGCCCCCTCGGGGCAGATCAGAGGCTCCCGATTCCCTCAATGGCCCACTGGGCCCCAGTGAG
  GCTAGCATTGCCAGCAATCTACAAGCCATGTCCACCCAGATGTTCCAGACTAAGCGCTGCTTCCGACTGG
  CCCCCACCTTTAGCAACCTGCTCCTCCAGCCCACCACCAACCCTCATACCTCGGCCAGCCACAGGCCTTG
  CGTCAATGGGGATGTAGACAAGCCTTCAGAGCCAGCCTCTGAGGAGGGCTCTGAGTCGGAGGGGAGTGAG
  TCCAGTGGACGCTCCTGTCGGAATGAGCGCAGCATCCAGGAGAAGCTTCAGGTCCTGATGGCCGAAGGTC
  TGCTTCCTGCTGTGAAAGTCTTCCTGGACTGGCTTCGGACCAACCCCGACCTCATCATCGTGTGTGCGCA
  GAGCTCTCAAAGTCTGTGGAACCGCCTGTCTGTGTTGCTGAATCTGTTGCCTGCTGCTGGTGAACTCCAG
  GAGTCTGGCCTGGCCTTGTGTCCTGAGGTCCAAGATCTTCTTGAAGGTTGTGAACTGCCTGACCTCCCCT
  CTAGCCTTCTGCTCCCAGAGGACATGGCTCTTCGTAACCTGCCCCCGCTCCGAGCTGCCCACAGACGCTT
  TAACTTTGACACGGATCGGCCCCTGCTCAGCACCTTAGAGGAGTCAGTGGTGCGCATCTGCTGCATCCGC
  AGCTTTGGTCATTTCATCGCCCGCCTGCAAGGCAGCATCCTGCAGTTCAACCCAGAGGTTGGCATCTTCG
  TCAGCATTGCCCAGTCTGAGCAGGAGAGCCTGCTGCAGCAGGCCCAGGCACAGTTCCGAATGGCACAGGA
  GGAAGCTCGTCGGAACAGGCTCATGAGAGACATGGCTCAGCTACGACTTCAGCTCGAAGTGTCTCAGCTG
  GAGGGCAGCCTGCAGCAGCCCAAGGCCCAGTCAGCCATGTCTCCCTACCTCGTCCCTGACACCCAGGCCC
  TCTGCCACCATCTCCCTGTCATCCGCCAACTGGCCACCAGTGGCCGCTTCATTGTCATCATCCCAAGGAC
  AGTGATCGATGGCCTGGATTTGCTGAAGAAGGAACACCCAGGGGCCCGGGATGGGATTCGGTACCTGGAG
  GCAGAGTTTAAAAAAGGAAACAGGTACATTCGCTGCCAGAAAGAGGTGGGAAAGAGCTTTGAGCGGCATA
  AGCTGAAGAGGCAGGATGCAGATGCCTGGACTCTCTATAAGATCCTAGACAGCTGCAAACAGCTGACTCT
  GGCCCAGGGGGCAGGTGAGGAGGATCCGAGTGGCATGGTGACCATCATCACAGGCCTTCCACTGGACAAC
  CCCAGCGTGCTTTCAGGCCCCATGCAGGCAGCCCTGCAGGCCGCTGCCCACGCCAGTGTGGACATCAAGA
  ATGTTCTGGACTTCTACAAGCAGTGGAAGGAAATTGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206137 representing NM_015327
  Red=Cloning site Green=Tags(s)
  
  MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLH
  PVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCC
  IDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQ
  IGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKR
  CKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFL
  PDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDE
  PESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARAS
  EGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSE
  ASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSE
  SSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQ
  ESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIR
  SFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQL
  EGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLE
  AEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDN
  PSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015327
• ORF Size: 3048 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015327.1, NM_015327.2, NP_056142.1, NP_056142.2
• RefSeq Size: 4577
• RefSeq ORF: 3051
• Locus ID: 23381
• Domains: PINc
• MW: 113.9 kDa
• Gene Summary: SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]). [supplied by OMIM, Mar 2008]