HIPK1 (NM_198269) Human Tagged ORF Clone

CAT#: RC206203

HIPK1 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 1 (HIPK1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY404996) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206203 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIPK1
• Synonyms: Myak; Nbak2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206203 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTCACTGGGCTGTGTGATAGCTGAGCTGTTCCTGGGATGGCCTCTTTATCCTGGTGCTTCAGAAT
  ATGATCAGATTCGTTATATTTCACAAACACAAGGCTTGCCAGCTGAATATCTTCTCAGTGCCGGAACAAA
  AACAACCAGGTTTTTCAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGACACCTGAAGAA
  CATGAACTGGAGACTGGAATAAAATCAAAAGAAGCTCGGAAGTACATTTTTAATTGCTTAGATGACATGG
  CTCAGGTGAATATGTCTACAGACCTGGAGGGAACAGACATGTTGGCAGAGAAGGCAGACCGAAGAGAATA
  CATTGATCTGTTAAAGAAAATGCTCACAATTGATGCAGATAAGAGAATTACCCCTCTAAAAACTCTTAAC
  CATCAGTTTGTGACAATGACTCACCTTTTGGATTTTCCACATAGCAATCATGTTAAGTCTTGTTTTCAGA
  ACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGATACAGTGAGTCAGATCAAGAGTCCCTTCACTAC
  ACATGTTGCCCCAAATACAAGCACAAATCTAACCATGAGCTTCAGCAATCAGCTCAATACAGTGCACAAT
  CAGGCCAGTGTTCTAGCTTCCAGTTCTACTGCAGCAGCTGCTACTCTTTCTCTGGCTAATTCAGATGTCT
  CACTACTAAACTACCAGTCAGCTTTGTACCCATCATCTGCTGCACCAGTTCCTGGAGTTGCCCAGCAGGG
  TGTTTCCTTGCAGCCTGGAACCACCCAGATTTGCACTCAGACAGATCCATTCCAACAGACATTTATAGTA
  TGTCCACCTGCGTTTCAAACTGGACTACAAGCAACAACAAAGCATTCTGGATTCCCTGTGAGGATGGATA
  ATGCTGTACCAATTGTACCCCAGGCACCAGCTGCTCAGCCACTACAGATTCAGTCAGGAGTTCTCACGCA
  GGGAAGCTGTACACCACTAATGGTAGCAACTCTCCACCCTCAAGTAGCCACCATCACACCGCAGTATGCG
  GTGCCCTTTACTCTGAGCTGCGCAGCCGGCCGGCCGGCGCTGGTTGAACAGACTGCCGCTGTACTGCAGG
  CGTGGCCTGGAGGGACTCAGCAAATTCTCCTGCCTTCAACTTGGCAACAGTTGCCTGGGGTAGCTCTACA
  CAACTCTGTCCAGCCCACAGCAATGATTCCAGAGGCCATGGGGAGTGGACAGCAGCTAGCTGACTGGAGG
  AATGCCCACTCTCATGGCAACCAGTACAGCACTATCATGCAGCAGCCATCCTTGCTGACTAACCATGTGA
  CATTGGCCACTGCTCAGCCTCTGAATGTTGGTGTTGCCCATGTTGTCAGACAACAACAATCCAGTTCCCT
  CCCTTCGAAGAAGAATAAGCAGTCAGCTCCAGTCTCTTCCAAGTCCTCTCTAGATGTTCTGCCTTCCCAA
  GTCTATTCTCTGGTTGGGAGCAGTCCCCTCCGCACCACATCTTCTTATAATTCCTTGGTCCCTGTCCAAG
  ATCAGCATCAGCCCATCATCATTCCAGATACTCCCAGCCCTCCTGTGAGTGTCATCACTATCCGAAGTGA
  CACTGATGAGGAAGAGGACAACAAATACAAGCCCAGTAGCTCTGGACTGAAGCCAAGGTCTAATGTCATC
  AGTTATGTCACTGTCAATGATTCTCCAGACTCTGACTCTTCTTTGAGCAGCCCTTATTCCACTGATACCC
  TGAGTGCTCTCCGAGGCAATAGTGGATCCGTTTTGGAGGGGCCTGGCAGAGTTGTGGCAGATGGCACTGG
  CACCCGCACTATCATTGTGCCTCCACTGAAAACTCAGCTTGGTGACTGCACTGTAGCAACCCAGGCCTCA
  GGTCTCCTGAGCAATAAGACTAAGCCAGTCGCTTCAGTGAGTGGGCAGTCATCTGGATGCTGTATCACCC
  CCACAGGGTATCGAGCTCAACGCGGGGGGACCAGTGCAGCACAACCACTCAATCTTAGCCAGAACCAGCA
  GTCATCGGCGGCTCCAACCTCACAGGAGAGAAGCAGCAACCCAGCCCCCCGCAGGCAGCAGGCGTTTGTG
  GCCCCTCTCTCCCAAGCCCCCTACACCTTCCAGCATGGCAGCCCGCTACACTCGACAGGGCACCCACACC
  TTGCCCCGGCCCCTGCTCACCTGCCAAGCCAGGCTCATCTGTATACGTATGCTGCCCCGACTTCTGCTGC
  TGCACTGGGCTCAACCAGCTCCATTGCTCATCTTTTCTCCCCACAGGGTTCCTCAAGGCATGCTGCAGCC
  TATACCACTCACCCTAGCACTTTGGTGCACCAGGTCCCTGTCAGTGTTGGGCCCAGCCTCCTCACTTCTG
  CCAGCGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACCCAATCCTACATTGGGTCTTCCCGAGGCTC
  AACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGCCAGTATTCCTACTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206203 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEE
  HELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLN
  HQFVTMTHLLDFPHSNHVKSCFQNMEICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHN
  QASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIV
  CPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYA
  VPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSTWQQLPGVALHNSVQPTAMIPEAMGSGQQLADWR
  NAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLDVLPSQ
  VYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPSSSGLKPRSNVI
  SYVTVNDSPDSDSSLSSPYSTDTLSALRGNSGSVLEGPGRVVADGTGTRTIIVPPLKTQLGDCTVATQAS
  GLLSNKTKPVASVSGQSSGCCITPTGYRAQRGGTSAAQPLNLSQNQQSSAAPTSQERSSNPAPRRQQAFV
  APLSQAPYTFQHGSPLHSTGHPHLAPAPAHLPSQAHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAA
  YTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198269
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198269.1, NM_198269.2, NP_938010.1
• RefSeq Size: 7066 bp
• RefSeq ORF: 2511 bp
• Locus ID: 204851
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• MW: 89.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]