EXDL1 (EXD1) (NM_152596) Human Tagged ORF Clone

CAT#: RC206223

EXD1 (Myc-DDK-tagged)-Human exonuclease 3'-5' domain containing 1 (EXD1)

Product Images

Western blot validation of overexpression lysate (Cat# LY407402) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206223 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EXD1 protein (Cat# TP306223). The protein was produced from HEK293T cells transfected with EXD1 cDNA clone (Cat# RC206223) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXD1
• Synonyms: EXDL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206223 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACAGTGAATTCCTAGCTTATGTGGAACTACTAGATGAAGTGGAACAAGGCTCAGTGAGAGCAA
  AAGCATCTTCTGTTAGTCTACATGCAGAAAGAACCTGGATGGAGAAAATGAAAGTTGAAGACCTAAATGT
  ATGTGAGCCTGCTTCTCCTGCCCCTGAAGCACCAGCTACCTCTCTGCTGAATGACCTCAAGTACAGCCCA
  TCAGAGGAAGAGGAGGTGACATACACAGTCATTAATCAATTCCAGCAGAAGTTTGGTGCTGCGATACTCC
  ATATCAAGAAGCAGAATGTCCTGAGTGTGGCAGCAGAAGGAGCGAATGTATGTCGCCATGGCAAACTGTG
  CTGGCTGCAGGTGGCCACAAATTGCCGAGTTTACTTATTTGACATTTTCCTTCTGGGAAGTCGAGCTTTC
  CACAATGGACTTCAGATGATACTAGAAGACAAGAGAATTTTGAAGGTTATCCATGATTGTCGTTGGCTTT
  CTGATTGCCTCTCTCATCAGTATGGAATTTTGCTGAATAATGTCTTTGACACACAGGTAGCAGATGTACT
  TCAGTTTTCCATGGAAACGGGTGGCTATCTTCCAAACTGCATCACTACTTTGCAGGAGAGTTTAATCAAA
  CACCTTCAAGTAGCCCCTAAATATCTCTCCTTTCTAGAAAAGAGACAAAAACTAATTCAGGAAAATCCAG
  AAGTATGGTTCATCCGACCTGTTTCACCCTCTTTACTGAAAATTTTGGCCCTGGAAGCTACCTACCTGTT
  ACCCCTTCGCTTGGCACTCCTAGATGAGATGATGTCTGACCTAACCACCCTGGTGGATGGTTACCTAAAC
  ACGTATCGCGAAGGGTCTGCAGACCGGCTTGGAGGCACTGAGCCTACATGTATGGAGCTGCCAGAGGAAC
  TGCTTCAACTCAAGGACTTCCAGAAGCAGCGCAGGGAGAAAGCTGCAAGAGAATATAGGGTGAATGCACA
  GGGACTCCTGATAAGGACAGTGCTACAGCCAAAGAAATTAGTGACAGAGACAGCAGGGAAAGAGGAGAAA
  GTCAAAGGCTTCTTATTTGGTAAAAATTTTAGGATAGATAAAGCTCCAAGTTTTACATCTCAAGACTTTC
  ACGGGGATGTGAATTTACTGAAAGAAGAATCTTTGAATAAACAAGCTACAAATCCTCAACATCTACCTCC
  CACGGAGGAAGGGGAAACTAGTGAGGATTCCAGTAACAAACTCATTTGCACAAAGTCAAAGGGGTCAGAG
  GACCAGAGAATAACTCAGAAAGAACACTTTATGACACCCAAACATGAGTTTCAGGCAAGTTTATCTTTGA
  AAGAGGAGACAGAACAGTTATTGATGGTGGAAAACAAGGAAGATTTAAAATGCACAAAACAGGCTGTTTC
  AATGTCTTCCTTTCCTCAGGAAACCAGAGTGTCTCCAAGTGACACTTTTTATCCTATCAGAAAGACTGTG
  GTTTCCACACTCCCTCCCTGTCCAGCCTTGGAGAAGATCGATTCCTGGATAAGTCCTTTTCTAAATCTGC
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206223 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSEFLAYVELLDEVEQGSVRAKASSVSLHAERTWMEKMKVEDLNVCEPASPAPEAPATSLLNDLKYSP
  SEEEEVTYTVINQFQQKFGAAILHIKKQNVLSVAAEGANVCRHGKLCWLQVATNCRVYLFDIFLLGSRAF
  HNGLQMILEDKRILKVIHDCRWLSDCLSHQYGILLNNVFDTQVADVLQFSMETGGYLPNCITTLQESLIK
  HLQVAPKYLSFLEKRQKLIQENPEVWFIRPVSPSLLKILALEATYLLPLRLALLDEMMSDLTTLVDGYLN
  TYREGSADRLGGTEPTCMELPEELLQLKDFQKQRREKAAREYRVNAQGLLIRTVLQPKKLVTETAGKEEK
  VKGFLFGKNFRIDKAPSFTSQDFHGDVNLLKEESLNKQATNPQHLPPTEEGETSEDSSNKLICTKSKGSE
  DQRITQKEHFMTPKHEFQASLSLKEETEQLLMVENKEDLKCTKQAVSMSSFPQETRVSPSDTFYPIRKTV
  VSTLPPCPALEKIDSWISPFLNLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152596
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152596.2, NM_152596.3, NP_689809.2
• RefSeq Size: 3009 bp
• RefSeq ORF: 1545 bp
• Locus ID: 161829
• MW: 58.3 kDa
• Gene Summary: RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity (By similarity). The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease. [UniProtKB/Swiss-Prot Function]