Acetoacetyl CoA synthetase (AACS) (NM_023928) Human Tagged ORF Clone

CAT#: RC206247

  • TrueORF®

AACS (Myc-DDK-tagged)-Human acetoacetyl-CoA synthetase (AACS)


  "NM_023928" in other vectors (6)

Reconstitution Protocol

USD 610.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol AACS
Synonyms ACSF1; SUR-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206247 representing NM_023928
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAAGGAGGAGCGCCCCGGTCGGGAGGAGATCCTGGAGTGCCAGGTGATGTGGGAGCCTGACAGTA
AGAAGAACACGCAGATGGACCGCTTCCGGGCGGCTGTGGGCGCCGCCTGCGGCCTGGCGCTGGAGAGTTA
TGATGACTTGTACCATTGGTCCGTTGAGTCATATTCAGACTTCTGGGCAGAGTTCTGGAAATTCAGTGGA
ATTGTCTTCTCACGTGTGTATGATGAGGTTGTGGACACATCGAAAGGAATCGCAGATGTCCCCGAGTGGT
TCAAAGGCAGTCGGCTCAACTATGCAGAAAACCTCCTGCGGCACAAAGAGAATGACAGAGTTGCCCTTTA
CATTGCAAGGGAAGGCAAAGAGGAAATTGTGAAGGTGACTTTTGAAGAGCTGAGGCAAGAAGTGGCTTTG
TTTGCAGCAGCAATGAGGAAAATGGGTGTGAAGAAAGGAGATCGGGTTGTTGGTTATTTACCCAACAGTG
AGCACGCTGTCGAGGCGATGCTGGCTGCGGCAAGCATTGGTGCCATCTGGAGCTCCACGTCCCCGGACTT
CGGTGTGAATGGTGTGCTGGACCGGTTTTCTCAAATTCAGCCAAAGCTCATCTTCTCTGTGGAGGCTGTT
GTCTATAATGGCAAAGAGCACAACCACATGGAAAAGCTGCAGCAGGTGGTTAAAGGCCTACCAGACTTGA
AGAAAGTGGTGGTGATTCCTTATGTGTCCTCCAGAGAGAACATAGACCTTTCAAAGATTCCAAACAGTGT
GTTTCTGGATGACTTTCTTGCCACCGGCACCAGTGAGCAGGCCCCGCAGCTGGAGTTCGAGCAGCTGCCC
TTCAGCCACCCACTGTTCATCATGTTCTCATCGGGCACCACGGGCGCACCCAAGTGCATGGTGCATTCCG
CTGGGGGCACCCTCATCCAGCATCTGAAGGAGCACCTGCTGCACGGCAACATGACCAGCAGTGACATCCT
CCTGTGCTACACCACGGTCGGCTGGATGATGTGGAACTGGATGGTGTCCCTTCTGGCCACAGGAGCGGCC
ATGGTCTTGTACGATGGCTCCCCCCTGGTGCCCACGCCCAATGTGCTCTGGGACCTGGTTGACAGGATAG
GCATCACTGTCCTGGTAACTGGGGCCAAGTGGCTGTCAGTGCTGGAAGAGAAGGCCATGAAGCCGGTGGA
AACCCACAGTCTCCAGATGCTCCACACGATCCTGTCCACTGGCTCCCCACTGAAAGCCCAGAGCTACGAG
TATGTCTACAGGTGCATCAAGAGCAGCATCCTCCTGGGCTCCATCTCAGGAGGCACCGACATCATCTCCT
GCTTCATGGGCCACAATTTTTCTCTTCCTGTGTATAAAGGGGAGATTCAGGCCCGGAACCTGGGCATGGC
CGTGGAAGCGTGGAACGAGGAAGGAAAGGCGGTCTGGGGAGAGAGCGGCGAGCTGGTGTGTACTAAGCCG
ATCCCTTGCCAGCCCACACACTTCTGGAACGATGAGAACGGCAACAAGTACAGGAAGGCGTATTTCTCCA
AATTCCCAGGTATCTGGGCTCATGGCGACTACTGCAGAATCAACCCCAAGACCGGGGGCATCGTCATGCT
TGGCCGGAGTGACGGCACCCTCAACCCCAACGGGGTGCGGTTCGGCAGCTCGGAAATCTATAACATTGTG
GAATCCTTCGAGGAGGTGGAGGACAGCCTGTGTGTCCCCCAGTATAACAAGTACAGGGAGGAGAGGGTGA
TCCTCTTCCTGAAGATGGCCTCCGGGCACGCCTTCCAGCCTGACTTGGTTAAGAGGATCCGTGACGCCAT
CCGCATGGGCTTGTCTGCGCGACACGTGCCCAGCCTCATCCTGGAAACCAAGGGCATCCCGTATACGCTC
AACGGCAAGAAAGTGGAAGTTGCCGTCAAACAGATCATCGCTGGAAAAGCCGTGGAGCAAGGAGGTGCTT
TCTCGAACCCCGAGACCCTGGATCTGTACCGGGACATCCCTGAGCTGCAGGGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206247 representing NM_023928
Red=Cloning site Green=Tags(s)

MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSG
IVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVAL
FAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAV
VYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLP
FSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAA
MVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYE
YVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKP
IPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIV
ESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTL
NGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_023928
ORF Size 2016 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_023928.1, NM_023928.2, NM_023928.3, NM_023928.4, NP_076417.2
RefSeq Size 3329 bp
RefSeq ORF 2019 bp
Locus ID 65985
Domains AMP-binding
Protein Pathways Butanoate metabolism
MW 75 kDa

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