KLHDC8A (NM_018203) Human Tagged ORF Clone

CAT#: RC206258

KLHDC8A (Myc-DDK-tagged)-Human kelch domain containing 8A (KLHDC8A)

Product Images

Western blot validation of overexpression lysate (Cat# LY413248) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206258 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHDC8A
• Synonyms: FLJ10748
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206258 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGCCGGGTCTACT
  GCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGACGACAACGGCGTCCCCATGGA
  CTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACCGCCTTGCCCCGGCTGCCCACAGCACGGGCG
  GGGGTGGCCGTCACCGCCCTGGGGAAGCGGATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCC
  TGAAGGTCGTGGAGATGTACAACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGC
  CATGGGCATTTCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
  CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACACATGCCCACCC
  CGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTGGGGGGACGACAGTCCAAGTA
  CGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGCTCCTGGACCAAGTTTCCCAACATTCCCTAT
  AAGCGGGCCTTCTCCAGCTTTGTGACCCTGGACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTC
  GCCTCTACCGGCAGCCAAAGTTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAA
  GATGGAACGATCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
  ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCACCCAGGGAAGA
  ACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCCAGCATAGTCGTCAAGAACTG
  CCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGACGCAGTGGAGGCCCTGTGTGTCTCTGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206258 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVPNVKDFQWKRLAPLPSRRVYCSLLETGGQVYAIGGCDDNGVPMDCFEVYSPEADQWTALPRLPTARA
  GVAVTALGKRIMVIGGVGTNQLPLKVVEMYNIDEGKWKKRSMLREAAMGISVTAKDYRVYAAGGMGLDLR
  PHNHLQHYDMLKDMWVSLAHMPTPRYAATSFLRGSKIYVLGGRQSKYAVNAFEVFDIETRSWTKFPNIPY
  KRAFSSFVTLDNHLYSLGGLRQGRLYRQPKFLRTMDVFDMEQGGWLKMERSFFLKKRRADFVAGSLSGRV
  IVAGGLGNQPTVLETAEAFHPGKNKWEILPAMPTPRCACSSIVVKNCLLAVGGVNQGLSDAVEALCVSDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018203
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018203.1, NM_018203.2, NP_060673.1
• RefSeq Size: 3044 bp
• RefSeq ORF: 1053 bp
• Locus ID: 55220
• Domains: Kelch
• MW: 38.9 kDa
• Gene Summary: This gene encodes a kelch domain-containing protein which is upregulated in cancer. Upregulated expression of the encoded protein may provide an alternative pathway for tumors to maintain aggressiveness in the absence of epidermal growth factor receptor dependence. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]