KLHDC8A (NM_018203) Human Tagged ORF Clone

CAT#: RG206258

KLHDC8A (GFP-tagged) - Human kelch domain containing 8A (KLHDC8A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHDC8A
• Synonyms: FLJ10748
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206258 representing NM_018203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGCCGGGTCTACT
  GCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGACGACAACGGCGTCCCCATGGA
  CTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACCGCCTTGCCCCGGCTGCCCACAGCACGGGCG
  GGGGTGGCCGTCACCGCCCTGGGGAAGCGGATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCC
  TGAAGGTCGTGGAGATGTACAACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGC
  CATGGGCATTTCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
  CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACACATGCCCACCC
  CGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTGGGGGGACGACAGTCCAAGTA
  CGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGCTCCTGGACCAAGTTTCCCAACATTCCCTAT
  AAGCGGGCCTTCTCCAGCTTTGTGACCCTGGACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTC
  GCCTCTACCGGCAGCCAAAGTTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAA
  GATGGAACGATCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
  ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCACCCAGGGAAGA
  ACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCCAGCATAGTCGTCAAGAACTG
  CCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGACGCAGTGGAGGCCCTGTGTGTCTCTGACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206258 representing NM_018203
  Red=Cloning site Green=Tags(s)
  
  MEVPNVKDFQWKRLAPLPSRRVYCSLLETGGQVYAIGGCDDNGVPMDCFEVYSPEADQWTALPRLPTARA
  GVAVTALGKRIMVIGGVGTNQLPLKVVEMYNIDEGKWKKRSMLREAAMGISVTAKDYRVYAAGGMGLDLR
  PHNHLQHYDMLKDMWVSLAHMPTPRYAATSFLRGSKIYVLGGRQSKYAVNAFEVFDIETRSWTKFPNIPY
  KRAFSSFVTLDNHLYSLGGLRQGRLYRQPKFLRTMDVFDMEQGGWLKMERSFFLKKRRADFVAGSLSGRV
  IVAGGLGNQPTVLETAEAFHPGKNKWEILPAMPTPRCACSSIVVKNCLLAVGGVNQGLSDAVEALCVSDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018203
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018203.1, NP_060673.1
• RefSeq Size: 2500
• RefSeq ORF: 1053
• Locus ID: 55220
• Domains: Kelch
• Gene Summary: This gene encodes a kelch domain-containing protein which is upregulated in cancer. Upregulated expression of the encoded protein may provide an alternative pathway for tumors to maintain aggressiveness in the absence of epidermal growth factor receptor dependence. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]