STAM2 (NM_005843) Human Tagged ORF Clone

CAT#: RC206279

STAM2 (Myc-DDK-tagged)-Human signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (STAM2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401773) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206279 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STAM2 protein (Cat# TP306279). The protein was produced from HEK293T cells transfected with STAM2 cDNA clone (Cat# RC206279) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAM2
• Synonyms: Hbp; STAM2A; STAM2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206279 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTGTTCACCGCCAACCCCTTCGAGCAAGACGTGGAAAAAGCCACGAATGAGTACAACACTACAG
  AAGATTGGAGTCTTATTATGGACATATGTGACAAAGTTGGAAGTACTCCTAATGGAGCGAAAGATTGCCT
  AAAAGCCATAATGAAAAGGGTAAATCATAAGGTTCCACATGTTGCTCTGCAAGCACTAACTCTTCTTGGG
  GCTTGTGTGGCAAACTGTGGAAAGATATTTCATTTAGAAGTATGTTCCCGTGATTTTGCAACAGAAGTAC
  GTGCTGTGATTAAAAATAAGGCACATCCTAAAGTATGTGAAAAACTGAAATCTTTAATGGTGGAGTGGTC
  AGAAGAATTTCAGAAGGACCCTCAGTTTAGTCTGATATCTGCAACTATTAAATCTATGAAAGAAGAAGGA
  ATTACTTTTCCTCCAGCAGGTTCTCAGACTGTCTCAGCTGCTGCCAAGAATGGTACGTCATCGAACAAAA
  ACAAAGAGGATGAAGACATAGCTAAAGCTATTGAATTATCGCTGCAAGAACAGAAACAGCAACACACAGA
  AACAAAATCCTTATATCCATCTTCAGAAATTCAGTTAAATAATAAGGTTGCACGGAAAGTGAGAGCTTTA
  TATGATTTTGAAGCTGTTGAGGACAATGAACTCACCTTTAAACATGGTGAAATAATTATTGTTTTGGATG
  ACAGTGATGCCAATTGGTGGAAAGGAGAAAATCACAGAGGAATAGGACTTTTCCCATCCAATTTTGTAAC
  AACTAATTTAAACATAGAGACTGAGGCAGCGGCTGTGGACAAATTGAATGTAATTGATGATGATGTGGAG
  GAAATTAAGAAATCAGAGCCTGAGCCTGTTTATATAGATGAGGATAAGATGGATAGAGCCCTGCAGGTAC
  TTCAGAGTATAGATCCAACAGATTCAAAACCAGACTCCCAAGACCTTTTGGATTTAGAAGATATCTGCCA
  ACAGATGGGTCCAATGATAGATGAAAAACTTGAAGAAATTGATAGGAAGCATTCAGAATTGTCTGAATTG
  AATGTTAAAGTCCTGGAAGCTCTGGAACTATATAACAAATTGGTGAATGAAGCACCAGTGTACTCAGTCT
  ATTCAAAGCTCCACCCTCCAGCACATTACCCACCTGCATCATCTGGGGTTCCAATGCAGACATATCCAGT
  TCAATCACATGGTGGAAACTATATGGGTCAGAGCATTCACCAAGTAACTGTTGCCCAAAGCTATAGCCTA
  GGACCCGATCAAATTGGTCCACTGAGATCTCTGCCTCCAAATGTGAATTCCTCAGTGACAGCACAGCCTG
  CTCAAACTTCATATTTAAGCACTGGACAAGACACTGTTTCCAATCCTACTTATATGAACCAGAACTCTAA
  CCTACAGTCAGCTACTGGTACAACTGCTTACACACAGCAAATGGGGATGTCTGTGGATATGTCATCTTAT
  CAGAACACTACTTCCAATTTGCCTCAACTGGCAGGCTTTCCGGTGACAGTTCCAGCTCATCCAGTTGCAC
  AGCAGCACACAAATTACCATCAGCAGCCTCTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206279 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAIMKRVNHKVPHVALQALTLLG
  ACVANCGKIFHLEVCSRDFATEVRAVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEG
  ITFPPAGSQTVSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLNNKVARKVRAL
  YDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGLFPSNFVTTNLNIETEAAAVDKLNVIDDDVE
  EIKKSEPEPVYIDEDKMDRALQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSEL
  NVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSHGGNYMGQSIHQVTVAQSYSL
  GPDQIGPLRSLPPNVNSSVTAQPAQTSYLSTGQDTVSNPTYMNQNSNLQSATGTTAYTQQMGMSVDMSSY
  QNTTSNLPQLAGFPVTVPAHPVAQQHTNYHQQPLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005843
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005843.2, NM_005843.3, NM_005843.4, NM_005843.5, NP_005834.3
• RefSeq Size: 5701 bp
• RefSeq ORF: 1578 bp
• Locus ID: 10254
• Domains: SH3, VHS, UIM
• Protein Pathways: Endocytosis, Jak-STAT signaling pathway
• MW: 58.2 kDa
• Gene Summary: The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq, Jul 2008]