ARID3B (NM_006465) Human Tagged ORF Clone

CAT#: RC206328

ARID3B (Myc-DDK-tagged)-Human AT rich interactive domain 3B (BRIGHT-like) (ARID3B)

Product Images

Western blot validation of overexpression lysate (Cat# LY416624) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206328 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARID3B
• Synonyms: BDP; DRIL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206328 representing NM_006465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCACTTCAGCAGCAGCAGCAGCAGCAGCAGCAACAACAGAAGCAGCCACACCTGGCTCCTCTGC
  AGATGGATGCCAGAGAGAAGCAGGGCCAGCAGATGAGAGAAGCCCAGTTCTTGTATGCCCAAAAGCTGGT
  CACACAGCCGACTCTCCTTTCCGCCACAGCTGGGAGACCTTCTGGCAGCACTCCCTTAGGTCCCTTAGCC
  AGAGTTCCACCCACCGCAGCAGTGGCCCAAGTGTTTGAACGGGGCAACATGAACTCAGAGCCTGAGGAAG
  AGGACGGAGGTTTGGAAGATGAGGATGGGGATGATGAAGTTGCAGAGGTGGCTGAGAAAGAAACCCAGGC
  TGCTTCAAAATATTTTCATGTGCAGAAAGTAGCTCGCCAAGATCCCAGAGTGGCACCCATGTCCAATCTA
  CTTCCAGCACCAGGGCTCCCACCACATGGACAACAAGCTAAAGAAGACCATACCAAAGATGCTTCCAAGG
  CCTCACCTTCTGTCTCCACAGCAGGACAGCCGAACTGGAATCTGGATGAGCAGCTCAAGCAGAATGGTGG
  TTTGGCCTGGAGTGATGATGCAGATGGAGGCCGGGGAAGAGAGATCTCTCGAGATTTTGCCAAGCTGTAT
  GAACTGGACGGTGATCCTGAAAGGAAAGAGTTCCTGGATGACCTCTTCGTCTTTATGCAGAAGAGGGGGA
  CCCCCATCAACCGAATCCCCATCATGGCCAAACAGATCCTGGACCTGTACATGCTGTATAAGCTGGTGAC
  CGAGAAGGGAGGCCTGGTGGAGATCATCAACAAGAAGATCTGGAGGGAGATCACCAAAGGCCTAAACCTG
  CCCACATCCATCACCAGCGCCGCCTTCACCCTCAGGACGCAGTACATGAAGTATCTGTATGCCTATGAGT
  GTGAGAAGAAAGCCTTGAGTTCCCCAGCCGAGCTCCAGGCAGCAATTGATGGCAACCGCAGGGAGGGCCG
  GCGGCCCAGCTACAGCTCCTCCCTCTTTGGCTACTCACCTGCTGCGGCTACTGCTGCTGCCGCTGCCGGG
  GCCCCTGCCCTTCTCTCCCCACCCAAGATCCGCTTTCCCATCCTTGGGCTTGGCTCCAGCAGTGGTACCA
  ATACCAGTAGCCCTCGGATATCCCCAGCAACCACTCTCAGGAAAGGTGATGGAGCCCCAGTGACAACAGT
  GCCTGTGCCAAATCGTCTGGCTGTGCCCGTGACCTTGGCAAGCCAGCAGGCTGGTACTCGGACCGCCGCA
  CTGGAGCAGCTGCGGGAGCGGCTGGAGTCAGGGGAGCCTGCTGAGAAGAAGGCATCGAGGCTGTCTGAGG
  AGGAGCAGCGCCTGGTGCAGCAGGCCTTCCAGCGCAACTTTTTCAGCATGGCACGGCAGCTCCCCATGAA
  GATCAGGATCAACGGCAGGGAAGACAGAGCAGAGGCCTCGGCTGCAGCACTGAACCTGACCACGAGTAGC
  ATTGGGAGCATTAACATGTCTGTGGACATCGATGGCACCACCTATGCAGGTGTGCTGTTTGCCCAGAAGC
  CTGTGGTCCACCTCATCACGGGGTCTGCTCCCCAGAGCCTCGGCAGCAGCGCCAGCAGCAGCAGCAGCTC
  TCACTGTTCACCAAGTCCTACCTCATCCCGGGGCACCCCCAGCGCAGAGCCCTCCACCAGCTGGTCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206328 representing NM_006465
  Red=Cloning site Green=Tags(s)
  
  MEPLQQQQQQQQQQQKQPHLAPLQMDAREKQGQQMREAQFLYAQKLVTQPTLLSATAGRPSGSTPLGPLA
  RVPPTAAVAQVFERGNMNSEPEEEDGGLEDEDGDDEVAEVAEKETQAASKYFHVQKVARQDPRVAPMSNL
  LPAPGLPPHGQQAKEDHTKDASKASPSVSTAGQPNWNLDEQLKQNGGLAWSDDADGGRGREISRDFAKLY
  ELDGDPERKEFLDDLFVFMQKRGTPINRIPIMAKQILDLYMLYKLVTEKGGLVEIINKKIWREITKGLNL
  PTSITSAAFTLRTQYMKYLYAYECEKKALSSPAELQAAIDGNRREGRRPSYSSSLFGYSPAAATAAAAAG
  APALLSPPKIRFPILGLGSSSGTNTSSPRISPATTLRKGDGAPVTTVPVPNRLAVPVTLASQQAGTRTAA
  LEQLRERLESGEPAEKKASRLSEEEQRLVQQAFQRNFFSMARQLPMKIRINGREDRAEASAAALNLTTSS
  IGSINMSVDIDGTTYAGVLFAQKPVVHLITGSAPQSLGSSASSSSSSHCSPSPTSSRGTPSAEPSTSWSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006465
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006465.1, NM_006465.2, NM_006465.3, NP_006456.1
• RefSeq Size: 4253
• RefSeq ORF: 1683
• Locus ID: 10620
• Protein Families: Stem cell - Pluripotency
• MW: 60.4 kDa
• Gene Summary: This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA-binding proteins. The encoded protein is homologous with two proteins that bind to the retinoblastoma gene product, and also with the mouse Bright and Drosophila dead ringer proteins. A pseudogene on chromosome 1p31 exists for this gene. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]