C11orf82 (DDIAS) (NM_145018) Human Tagged ORF Clone

CAT#: RC206347

C11orf82 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 82 (C11orf82)


  "NM_145018" in other vectors (4)

Reconstitution Protocol

USD 750.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DDIAS
Synonyms C11orf82; noxin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206347 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGAAGACGAAAATTTCTTCTAGCCTCAGTACTTGCTCTCCAGAATTCAAGTTTTATATATCCAT
CATGTCAGAAGTGCTTCTCTAGGATAATCCTGGTCTCCAAAAGGTCTAATTGTCCAAAATGTGGCTCTAC
TGGTGAATCTGGAAATGCCAATTACAGATACAAACTTTCCTTAAAAGTTGCAGAATCAAACAAATTGTTT
GTTATTACTGTATTTGGAAGTTGCTTAGATACATTTTTTGGTCTTACTGCCACTGGTTTGCACAGGTACA
TTCAGGATCCTAATAAAATTCCAGAAACACTGGACAATGATACAACTCAGAATCTATTAACTAAAGCAGT
TGAAACTTGCTTTGTTGGACAAAGCTTTATTTTTGGAGTGACGAATTTTGAAAACCAACCTGGACAAGGT
TCAGATGCCAGTAACTTCTTACAGCAATGCTCTGACCACAAAAGAAAAGCCAAAGCACTAGTGGCTTGCC
AGATTGTTCTACCAGACCCAGGTATTGCAGGCTTTACTGTCATTGACTACTTCCATCAACTTTTGCAGAC
TTTTAATTTCAGGAAACTTCAGTGTGACTCTCAGGCACCTAACAATCACTTACTTGCTTTAGATCACTCA
AATAGTGATCTCAGCAGCACATATACTTCTGACAGCACTTCTGATTTTTTCAAGTCCTGCAGCAAGGATA
CTTTTTCAAAATTCTGGCAGCCATCACTTGAATTCACTTGCATTGTTTCACAACTAACAGATAATGATGA
TTTTTCAGCTTCAGAACAAAGTAAGGCCTTTGGTACTCTTCAGCAGAACAGAAAGTCCATCTCCATTGCA
GAGGCCACTGGTTCCAGTAGCTGCCATGATCCCATTCAGGATTCATGGAGCCTTGTTTCATATATGGATA
AAAAGAGTACAGCAGAAAAGTTGGGTAAAGAACTTGGCTTACAAGCTAAGGAGCTGAGTGCAGTTCACAG
CAGTCATCATGAAATTGGAGTTAATGACTCTAATTTATTCTCTTTGGAAATGCGAGAGCCCCTTGAGTCA
AGTAATACAAAATCCTTCCACAGTGCAGTGGAAATTAAAAATAGGTCCCAGCATGAGCTACCATGTTTTC
AGCATCATGGTATAGATACCCCAACTAGCCTTCAGAAGAGATCTGCATGTTGTCCACCTTCGTTACTCAG
ACTTGAAGAGACAGCCAGCAGTTCCCAGGATGGTGACCCTCAAATTTGGGATGATCTGCCATTCTCTGAA
AGCCTGAACAAGTTTCTGGCAGTTCTTGAAAGTGAGATTGCTGTAACCCAGGCAGATGTCAGTAGTAGGA
AACATCATGTAGATAATGACATTGATAAATTTCATGCAGACCACAGCAGTTTATCTGTGACTCCCCAGAG
AACTACTGGAGCCCTGCATACACCACCTATAGCTTTAAGATCATCACAAGTAATAGTCAAAGCAAACTGT
AGCAAAGATGACTTCCTTTTCAACTGTAAAGGAAATCTAAGTCCTAGTGTTGAAAAGGAGTCACAACCAG
ATAACAAAGTAGAGGCTGTCTCTGTAAATCATAATGGAAGAGATATGTCAGAATATTTTTTACCGAATCC
TTACCTGTCAGCTCTGTCTTCATCTTCAAAAGATTTAGAAACAATAGTTACTCTTAAGAAGACTATCAGA
ATCTCACCACACAGGGAGAGTGACCATTCTAGTCTAAATAACAAATATTTGAATGGATGTGGAGAAATAT
CAGTTTCAGAAATGAATGAAAAGTTGACAACTCTGTGTTATAGGAAGTATAATGATGTCTCTGATCTTTG
CAAATTAGAAAATAAACAATATTGTAGGTGGTCCAAGAACCAAGATGACAGTTTTACAATTTGCAGGAAA
CTTACATATCCTTTAGAAACTCTTTGCAATAGTCCAAATAGAAGTACAAATACATTGAAAGAAATGCCTT
GGGGACATATCAATAACAACGTAACACAGAGCTATTCTATTGGTTATGAAGGTAGCTATGATGCCTCTGC
TGATCTCTTTGATGATATTGCTAAAGAAATGGACATTGCAACTGAGATTACCAAAAAATCACAGGATATT
TTGTTAAAATGGGGAACATCTTTGGCAGAAAGTCACCCTTCAGAGTCTGATTTTTCACTGAGATCACTTT
CTGAAGACTTCATCCAGCCTTCACAAAAATTATCCTTGCAAAGCCTATCTGACTCTAGGCATTCAAGAAC
ATGCTCTCCAACACCTCATTTTCAATCAGATTCAGAATATAATTTTGAAAATAGTCAAGACTTTGTTCCA
TGTTCACAGTCAACTCCAATTTCAGGGTTCCACCAAACAAGAATTCATGGGATAAACAGAGCTTTCAAAA
AACCTGTATTTTATTCAGATCTTGATGGTAACTATGAAAAAATAAGGATTTTCCCTGAAAATGACAAACA
GCAAGCCAGCCCAAGCTGTCCAAAAAATATAAAAACACCTAGCCAGAAAATCAGAAGCCCTATTGTATCT
GGTATTTCACAACCAGACGTTTTCAATCACTACCCTTTTGCTGAGTGCCATGAAACTGATAGTGATGAAT
GGGTCCCTCCTACCACACAAAAAATATTTCCTTCAGATATGCTTGGATTCCAAGGCATAGGTCTAGGGAA
ATGCCTTGCTGCCTATCATTTCCCTGATCAACAAGAGTTACCAAGAAAGAAACTGAAACATATTAGACAA
GGAACCAATAAAGGTTTAATTAAGAAGAAATTAAAGAATATGCTTGCAGCAGTTGTTACGAAAAAGAAAA
CTCATAAATATAACTGTAAAAGTTCAGGCTGGATTTCCAAATGTCCAGACATTCAAGTCTTAGCAGCACC
TCAGCTGCACCCTATTCTTGGACCTGATTCTTGTTCAGAAGTCAAATGTTGCCTTCCATTTTCAGAAAAA
GGCCCACCTTCAGTGTGTGAAACTCGAAGTGCTTGGTCACCTGAATTGTTTTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206347 protein sequence
Red=Cloning site Green=Tags(s)

MNRRRKFLLASVLALQNSSFIYPSCQKCFSRIILVSKRSNCPKCGSTGESGNANYRYKLSLKVAESNKLF
VITVFGSCLDTFFGLTATGLHRYIQDPNKIPETLDNDTTQNLLTKAVETCFVGQSFIFGVTNFENQPGQG
SDASNFLQQCSDHKRKAKALVACQIVLPDPGIAGFTVIDYFHQLLQTFNFRKLQCDSQAPNNHLLALDHS
NSDLSSTYTSDSTSDFFKSCSKDTFSKFWQPSLEFTCIVSQLTDNDDFSASEQSKAFGTLQQNRKSISIA
EATGSSSCHDPIQDSWSLVSYMDKKSTAEKLGKELGLQAKELSAVHSSHHEIGVNDSNLFSLEMREPLES
SNTKSFHSAVEIKNRSQHELPCFQHHGIDTPTSLQKRSACCPPSLLRLEETASSSQDGDPQIWDDLPFSE
SLNKFLAVLESEIAVTQADVSSRKHHVDNDIDKFHADHSSLSVTPQRTTGALHTPPIALRSSQVIVKANC
SKDDFLFNCKGNLSPSVEKESQPDNKVEAVSVNHNGRDMSEYFLPNPYLSALSSSSKDLETIVTLKKTIR
ISPHRESDHSSLNNKYLNGCGEISVSEMNEKLTTLCYRKYNDVSDLCKLENKQYCRWSKNQDDSFTICRK
LTYPLETLCNSPNRSTNTLKEMPWGHINNNVTQSYSIGYEGSYDASADLFDDIAKEMDIATEITKKSQDI
LLKWGTSLAESHPSESDFSLRSLSEDFIQPSQKLSLQSLSDSRHSRTCSPTPHFQSDSEYNFENSQDFVP
CSQSTPISGFHQTRIHGINRAFKKPVFYSDLDGNYEKIRIFPENDKQQASPSCPKNIKTPSQKIRSPIVS
GISQPDVFNHYPFAECHETDSDEWVPPTTQKIFPSDMLGFQGIGLGKCLAAYHFPDQQELPRKKLKHIRQ
GTNKGLIKKKLKNMLAAVVTKKKTHKYNCKSSGWISKCPDIQVLAAPQLHPILGPDSCSEVKCCLPFSEK
GPPSVCETRSAWSPELFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145018
ORF Size 2994 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145018.2, NM_145018.3, NP_659455.2
RefSeq Size 3577 bp
RefSeq ORF 2997 bp
Locus ID 220042
MW 111.5 kDa

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