TMEM25 (NM_032780) Human Tagged ORF Clone

CAT#: RC206385

TMEM25 (Myc-DDK-tagged)-Human transmembrane protein 25 (TMEM25), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409928) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM25 protein (Cat# TP306385). The protein was produced from HEK293T cells transfected with TMEM25 cDNA clone (Cat# RC206385) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM25
• Synonyms: FLJ14399
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206385 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCCTCCAGGCCCAGCCGCCCTCCGGCACACACTGCTGCTCCTGCCAGCCCTTCTGAGCTCAG
  GTTGGGGGGAGTTGGAGCCACAAATAGATGGTCAGACCTGGGCTGAGCGGGCACTTCGGGAGAATGAACG
  CCACGCCTTCACCTGCCGGGTGGCAGGGGGGCCTGGCACCCCCAGATTGGCCTGGTATCTGGATGGACAG
  CTGCAGGAGGCCAGCACCTCAAGACTGCTGAGCGTGGGAGGGGAGGCCTTCTCTGGAGGCACCAGCACCT
  TCACTGTCACTGCCCATCGGGCCCAGCATGAGCTCAACTGCTCTCTGCAGGACCCCAGAAGTGGCCGATC
  AGCCAACGCCTCTGTCATCCTTAATGTGCAATTCAAGCCAGAGATTGCCCAAGTCGGCGCCAAGTACCAG
  GAAGCTCAGGGCCCAGGCCTCCTGGTTGTCCTGTTTGCCCTGGTGCGTGCCAACCCGCCGGCCAATGTCA
  CCTGGATCGACCAGGATGGGCCAGTGACTGTCAACACCTCTGACTTCCTGGTGCTGGATGCGCAGAACTA
  CCCCTGGCTCACCAACCACACGGTGCAGCTGCAGCTCCGCAGCCTGGCACACAACCTCTCGGTGGTGGCC
  ACCAATGACGTGGGTGTCACCAGTGCGTCGCTTCCAGCCCCAGGGCTTCTGGCTACCCGGGTGGAAGTGC
  CACTGCTGGGCATTGTTGTGGCTGCTGGGCTTGCACTGGGCACCCTCGTGGGGTTCAGCACCTTGGTGGC
  CTGCCTGGTCTGCAGAAAAGAGAAGAAAACCAAAGGCCCCTCCCGGCACCCATCTCTGATATCAAGTGAC
  TCCAACAACCTAAAACTCAACAACGTGCGCCTGCCACGGGAGAACATGTCCCTCCCGTCCAACCTTCAGC
  TCAATGACCTCACTCCAGATTCCAGAGCAGTGAAACCAGCAGACCGGCAGATGGCTCAGAACAACAGCCG
  GCCAGAGCTTCTGGACCCGGAGCCCGGCGGCCTCCTCACCAGCCAAGGTTTCATCCGCCTCCCAGTGCTG
  GGCTATATCTATCGAGTGTCCAGCGTGAGCAGTGATGAGATCTGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206385 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALPPGPAALRHTLLLLPALLSSGWGELEPQIDGQTWAERALRENERHAFTCRVAGGPGTPRLAWYLDGQ
  LQEASTSRLLSVGGEAFSGGTSTFTVTAHRAQHELNCSLQDPRSGRSANASVILNVQFKPEIAQVGAKYQ
  EAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNTSDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVA
  TNDVGVTSASLPAPGLLATRVEVPLLGIVVAAGLALGTLVGFSTLVACLVCRKEKKTKGPSRHPSLISSD
  SNNLKLNNVRLPRENMSLPSNLQLNDLTPDSRAVKPADRQMAQNNSRPELLDPEPGGLLTSQGFIRLPVL
  GYIYRVSSVSSDEIWL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032780
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032780.2, NM_032780.3, NP_116169.2
• RefSeq Size: 2551 bp
• RefSeq ORF: 1101 bp
• Locus ID: 84866
• Protein Families: Transmembrane
• MW: 39.3 kDa