FES (NM_002005) Human Tagged ORF Clone

CAT#: RC206408

FES (Myc-DDK-tagged)-Human feline sarcoma oncogene (FES), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419591) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206408 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FES
• Synonyms: FPS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206408 representing NM_002005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCTCTTCTGAGCTGTGCAGCCCCCAGGGCCACGGGGTCCTGCAGCAAATGCAGGAGGCCGAGC
  TTCGTCTACTGGAGGGCATGAGAAAGTGGATGGCCCAGCGGGTCAAGAGTGACAGGGAGTATGCAGGACT
  GCTTCACCACATGTCCCTGCAGGACAGTGGGGGCCAGAGCCGGGCCATCAGCCCTGACAGCCCCATCAGT
  CAGTCCTGGGCTGAGATCACCAGCCAAACTGAGGGCCTGAGCCGCTTGCTGCGGCAGCACGCAGAGGATC
  TGAACTCAGGGCCCCTGAGCAAGCTGAGCCTGCTCATCCGGGAACGGCAGCAGCTTCGCAAGACCTACAG
  CGAGCAGTGGCAGCAGCTGCAGCAGGAGCTCACCAAGACCCACAGCCAGGACATTGAGAAGCTGAAGAGC
  CAGTACCGAGCTCTGGCACGGGACAGTGCCCAAGCCAAGCGCAAGTACCAGGAGGCCAGCAAAGACAAGG
  ACCGTGACAAGGCCAAGGACAAGTATGTGCGCAGCCTGTGGAAGCTCTTTGCTCACCACAACCGCTATGT
  GCTGGGCGTGCGGGCTGCGCAGCTACACCACCAGCACCACCACCAGCTCCTGCTGCCCGGCCTGCTGCGG
  TCACTGCAGGACCTGCACGAGGAGATGGCTTGCATCCTGAAGGAGATCCTGCAGGAATACCTGGAGATTA
  GCAGCCTGGTGCAGGATGAGGTGGTGGCCATTCACCGGGAGATGGCTGCAGCTGCTGCCCGCATCCAGCC
  TGAGGCTGAGTACCAAGGCTTCCTGCGACAGTATGGGTCCGCACCTGACGTCCCACCCTGTGTCACGTTC
  GATGAGTCACTGCTTGAGGAGGGTGAACCGCTGGAGCCTGGGGAGCTCCAGCTGAACGAGCTGACTGTGG
  AGAGCGTGCAGCACACGCTGACCTCAGTGACAGATGAGCTGGCTGTGGCCACCGAGATGGTGTTCAGGCG
  GCAGGAGATGGTTACGCAGCTGCAACAGGAGCTCCGGAATGAAGAGGAGAACACCCACCCCCGGGAGCGG
  GTGCAGCTGCTGGGCAAGAGGCAAGTGCTGCAAGAAGCACTGCAGGGGCTGCAGGTAGCGCTGTGCAGCC
  AGGCCAAGCTGCAGGCCCAGCAGGAGTTGCTGCAGACCAAGCTGGAGCACCTGGGCCCCGGCGAGCCCCC
  GCCTGTGCTGCTCCTGCAGGATGACCGCCACTCCACGTCGTCCTCGGAGCAGGAGCGAGAGGGGGGAAGG
  ACACCCACGCTGGAGATCCTTAAGAGCCACATCTCAGGAATCTTCCGCCCCAAGTTCTCGCTCCCTCCAC
  CGCTGCAGCTCATTCCGGAGGTGCAGAAGCCCCTGCATGAGCAGCTGTGGTACCACGGGGCCATCCCGAG
  GGCAGAGGTGGCTGAGCTGCTGGTGCACTCTGGGGACTTCCTGGTGCGGGAGAGCCAGGGCAAGCAGGAG
  TACGTGCTGTCGGTGCTGTGGGATGGTCTGCCCCGGCACTTCATCATCCAGTCCTTGGATAACCTGTACC
  GACTGGAAGGGGAAGGCTTTCCTAGCATTCCTTTGCTCATCGACCACCTACTGAGCACCCAGCAGCCCCT
  CACCAAGAAGAGTGGTGTTGTCCTGCACAGGGCTGTGCCCAAGGACAAGTGGGTGCTGAACCATGAGGAC
  CTGGTGTTGGGTGAGCAGATTGGACGGGGGAACTTTGGCGAAGTGTTCAGCGGACGCCTGCGAGCCGACA
  ACACCCTGGTGGCGGTGAAGTCTTGTCGAGAGACGCTCCCACCTGACCTCAAGGCCAAGTTTCTACAGGA
  AGCGAGGATCCTGAAGCAGTACAGCCACCCCAACATCGTGCGTCTCATTGGTGTCTGCACCCAGAAGCAG
  CCCATCTACATCGTCATGGAGCTTGTGCAGGGGGGCGACTTCCTGACCTTCCTCCGCACGGAGGGGGCCC
  GCCTGCGGGTGAAGACTCTGCTGCAGATGGTGGGGGATGCAGCTGCTGGCATGGAGTACCTGGAGAGCAA
  GTGCTGCATCCACCGGGACCTGGCTGCTCGGAACTGCCTGGTGACAGAGAAGAATGTCCTGAAGATCAGT
  GACTTTGGGATGTCCCGAGAGGAAGCCGATGGGGTCTATGCAGCCTCAGGGGGCCTCAGACAAGTCCCCG
  TGAAGTGGACCGCACCTGAGGCCCTTAACTACGGCCGCTACTCCTCCGAAAGCGACGTGTGGAGCTTTGG
  CATCTTGCTCTGGGAGACCTTCAGCCTGGGGGCCTCCCCCTATCCCAACCTCAGCAATCAGCAGACACGG
  GAGTTTGTGGAGAAGGGGGGCCGTCTGCCCTGCCCAGAGCTGTGTCCTGATGCCGTGTTCAGGCTCATGG
  AGCAGTGCTGGGCCTATGAGCCTGGGCAGCGGCCCAGCTTCAGCACCATCTACCAGGAGCTGCAGAGCAT
  CCGAAAGCGGCATCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206408 representing NM_002005
  Red=Cloning site Green=Tags(s)
  
  MGFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDSGGQSRAISPDSPIS
  QSWAEITSQTEGLSRLLRQHAEDLNSGPLSKLSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKS
  QYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLR
  SLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTF
  DESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRER
  VQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPVLLLQDDRHSTSSSEQEREGGR
  TPTLEILKSHISGIFRPKFSLPPPLQLIPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQE
  YVLSVLWDGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRAVPKDKWVLNHED
  LVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLPPDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQ
  PIYIVMELVQGGDFLTFLRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVLKIS
  DFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGASPYPNLSNQQTR
  EFVEKGGRLPCPELCPDAVFRLMEQCWAYEPGQRPSFSTIYQELQSIRKRHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002005
• ORF Size: 2466 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002005.1, NM_002005.2, NM_002005.3, NP_001996.1
• RefSeq Size: 2762 bp
• RefSeq ORF: 2469 bp
• Locus ID: 2242
• Cytogenetics: 15q26.1
• Domains: pkinase, SH2, FCH, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance
• MW: 93.3 kDa
• Gene Summary: 'This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]'