NXF3 (NM_022052) Human Tagged ORF Clone

CAT#: RC206433

NXF3 (Myc-DDK-tagged)-Human nuclear RNA export factor 3 (NXF3)

Product Images

Western blot validation of overexpression lysate (Cat# LY411743) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206433 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NXF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206433 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTGCCTTCAGGACACACTACGGGTCACACTGATCAAGTTGTTCAAAGAAGAGCAAGATGTTGGG
  ATATTTACCAAAGGAGATTTAGCAGTAGGTCTGAACCTGTCAATCCTGGCATGCATTCTTCATCCCATCA
  GCAGCAAGATGGAGATGCAGCAATGCATGGTGCCCACATGGACTCTCCAGTAAGATACACTCCCTATACT
  ATTTCACCCTATAATCGGAAAGGCAGTTTTCGTAAACAAGACCAAACCCACGTTAACATGGAGAGAGAGC
  AAAAACCTCCAGAGAGAAGAATGGAGGGGAACATGCCGGATGGGACCTTAGGGAGCTGGTTCAAGATCAC
  AGTTCCCTTTGGCATAAAATACAATGAGAAGTGGCTGCTGAATTTGATTCAGAATGAATGCAGTGTACCC
  TTCGTCCCAGTTGAGTTTCACTATGAAAACATGCATGCCAGCTTCTTTGTGGAGAATGCCAGCATCGCCT
  ATGCACTGAAGAATGTCAGTGGCAAGATTTGGGATGAGGATAATGAAAAGATATCAATCTTTGTCAACCC
  TGCTGGCATACCCCACTTTGTGCACAGGGAGCTGAAGTCAGAAAAGGTGGAGCAGATAAAGCTGGCCATG
  AACCAACAGTGTGATGTCTCCCAGGAAGCTCTTGATATCCAGAGACTCCCATTTTACCCAGACATGGTGA
  ACCGTGATACTAAGATGGCATCGAATCCTAGAAAGTGCATGGCTGCCTCCCTGGACGTCCATGAAGAGAA
  CATACCTACAGTGATGTCTGCAGGGGAGATGGACAAGTGGAAAGGGATAGAGCCAGGAGAGAAGTGTGCG
  GACAGAAGCCCAGTGTGCACGACCTTCTCGGATACCTCCAGCAACATAAACTCCATCCTGGAATTGTTCC
  CCAAATTATTATGCCTGGATGGCCAGCAGTCACCCAGAGCAACTTTATGTGGTACTGAAGCCCACAAGAG
  GTTACCAACCTGTAAGGGAAGCTTCTTTGGATCTGAGATGTTGAAGAATCTGGTCCTGCAATTCCTGCAG
  CAGTATTACTTGATCTATGACTCTGGAGATCGACAGGGTCTCCTTAGTGCTTACCACGATGAGGCCTGCT
  TCTCCCTGAGCATTCCCTTCAATCCTGAGGACTCAGCCCCGAGCAGCTTCTGCAAGTTCTTCAAGGATAG
  CAGGAATATAAAAATTCTCAAGGACCCCTACCTTCGGGGGGAGCTGCTGAAGCACACAAAACTTGATATT
  GTGGACTCCCTCAGTGCGTTGCCTAAAACTCAGCATGACCTCAGCTCCTTCCTGGTGGACATGTGGTACC
  AGACGGAATGGATGCTCTGCTTTTCTGTCAACGGGGTGTTCAAGGAAGTGGAAGGACAGTCTCAGGGTTC
  TGTTCTCGCCTTCACCCGGACCTTCATTGCTACCCCTGGCAGCAGTTCCAGTCTGTGCATCGTGAATGAC
  AAGCTCTTTGTGCGGGATACCAGCCACCAAGGGACCCAGAGTGCCTTGTTCACCCTAGTGCCCACAGCCT
  TCTCCAGCTCCGTGCCTGCCTTCTCCCAGGAGCAGCAGAAAATGCTGCCTTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206433 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLPSGHTTGHTDQVVQRRARCWDIYQRRFSSRSEPVNPGMHSSSHQQQDGDAAMHGAHMDSPVRYTPYT
  ISPYNRKGSFRKQDQTHVNMEREQKPPERRMEGNMPDGTLGSWFKITVPFGIKYNEKWLLNLIQNECSVP
  FVPVEFHYENMHASFFVENASIAYALKNVSGKIWDEDNEKISIFVNPAGIPHFVHRELKSEKVEQIKLAM
  NQQCDVSQEALDIQRLPFYPDMVNRDTKMASNPRKCMAASLDVHEENIPTVMSAGEMDKWKGIEPGEKCA
  DRSPVCTTFSDTSSNINSILELFPKLLCLDGQQSPRATLCGTEAHKRLPTCKGSFFGSEMLKNLVLQFLQ
  QYYLIYDSGDRQGLLSAYHDEACFSLSIPFNPEDSAPSSFCKFFKDSRNIKILKDPYLRGELLKHTKLDI
  VDSLSALPKTQHDLSSFLVDMWYQTEWMLCFSVNGVFKEVEGQSQGSVLAFTRTFIATPGSSSSLCIVND
  KLFVRDTSHQGTQSALFTLVPTAFSSSVPAFSQEQQKMLPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022052
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022052.1, NP_071335.1
• RefSeq Size: 1915 bp
• RefSeq ORF: 1596 bp
• Locus ID: 56000
• MW: 60.1 kDa
• Gene Summary: This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq, Jul 2008]