Amphiphysin (AMPH) (NM_001635) Human Tagged ORF Clone

CAT#: RC206620

AMPH (Myc-DDK-tagged)-Human amphiphysin (AMPH), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400614) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206620 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AMPH protein (Cat# TP306620). The protein was produced from HEK293T cells transfected with AMPH cDNA clone (Cat# RC206620) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AMPH
• Synonyms: AMPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206620 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACATCAAGACGGGCATCTTCGCCAAGAACGTCCAGAAGCGACTCAACCGCGCGCAGGAAAAGG
  TCCTCCAAAAGCTGGGGAAAGCTGATGAGACAAAAGACGAACAGTTCGAAGAATATGTCCAGAACTTCAA
  ACGGCAAGAAGCAGAGGGTACCAGACTTCAGCGAGAACTCCGAGGATATTTAGCAGCAATCAAAGGCATG
  CAGGAGGCCTCCATGAAGCTCACAGAGTCGCTGCATGAAGTCTATGAGCCTGACTGGTATGGGCGGGAAG
  ATGTGAAAATGGTTGGTGAGAAATGTGATGTGCTGTGGGAAGACTTCCATCAAAAACTCGTGGATGGGTC
  CTTGCTAACACTGGATACCTACCTGGGGCAATTTCCTGACATAAAGAATCGCATCGCCAAGCGCAGCAGG
  AAGCTAGTGGACTATGACAGTGCCCGCCACCATCTGGAAGCTCTGCAGAGCTCCAAGAGGAAGGATGAGA
  GTCGAATCTCTAAGGCAGAAGAAGAATTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTTAACGTTGACTT
  ACAAGAAGAGTTACCATCATTATGGTCAAGACGAGTTGGATTTTATGTTAATACTTTCAAAAACGTCTCC
  AGCCTTGAAGCCAAGTTTCATAAGGAAATTGCGGTGCTTTGCCACAAACTGTATGAAGTGATGACAAAAC
  TGGGTGACCAGCACGCCGACAAGGCCTTCACCATCCAAGGAGCGCCCAGTGATTCGGGTCCTCTCCGCAT
  TGCAAAGACACCATCACCGCCTGAGGAGCCTTCACCCCTCCCGAGCCCGACAGCAAGTCCAAATCATACA
  TTAGCACCTGCGTCTCCCGCACCAGCACGGCCTCGGTCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC
  CACCTCTACCTAAAGTCACCCCGACAAAGGAACTGCAGCAGGAGAACATCATCAGTTTCTTTGAGGACAA
  CTTTGTTCCAGAAATCAGTGTGACAACACCTTCCCAGAATGAAGTCCCTGAGGTGAAGAAAGAGGAGACT
  TTGCTGGATCTGGACTTTGATCCTTTCAAGCCCGAGGTGACACCTGCAGGTTCTGCTGGAGTGACCCACT
  CACCCATGTCTCAGACATTGCCCTGGGACCTATGGACGACAAGCACTGATTTGGTACAGCCGGCTTCTGG
  TGGTTCATTTAATGGATTCACACAGCCCCAGGATACTTCATTATTCACAATGCAGACAGACCAGAGTATG
  ATCTGCAACTTGGCTGAATCTGAACAGGCTCCACCCACAGAGCCAAAAGCAGAGGAGCCTCTGGCTGCTG
  TCACACCTGCCGTTGGTCTGGACCTTGGAATGGACACTCGGGCTGAGGAGCCAGTGGAGGAGGCAGTGAT
  CATACCTGGAGCTGATGCTGATGCAGCTGTTGGAACCTTGGTGTCAGCAGCTGAGGGGGCCCCAGGAGAG
  GAAGCAGAGGCGGAGACGGCCACTGTCCCTGCCGGGGAAGGAGTAAGTTTAGAGGAGGCCAAAATTGGAA
  CTGAAACCACTGAGGGTGCAGAGAGTGCCCAACCTGAAGCAGAGGAGCTCGAAGCAACAGTGCCTCAGGA
  GAAGGTCATTCCTTCGGTGGTCATAGAGCCTGCCTCCAACCATGAAGAGGAAGGAGAAAACGAAATAACT
  ATAGGTGCAGAGCCCAAGGAGACCACCGAGGACGCGGCTCCTCCGGGCCCCACCAGCGAGACACCGGAGC
  TGGCTACGGAGCAGAAGCCTATCCAGGACCCTCAGCCCACGCCTTCTGCACCAGCCATGGGGGCTGCTGA
  CCAGCTAGCATCTGCAAGGGAGGCCTCTCAGGAATTGCCTCCTGGCTTTCTCTACAAGGTGGAAACACTG
  CATGATTTTGAGGCAGCAAATTCTGATGAACTTACCTTACAAAGGGGTGATGTGGTGCTGGTGGTCCCCT
  CAGATTCAGAAGCTGATCAGGATGCAGGCTGGCTGGTGGGAGTGAAGGAATCAGACTGGCTTCAGTACAG
  AGACCTTGCCACCTACAAAGGCCTCTTTCCAGAGAACTTCACCCGACGCTTAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206620 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGM
  QEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSR
  KLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVS
  SLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHT
  LAPASPAPARPRSPSQTRKGPPVPPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEVPEVKKEET
  LLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSM
  ICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGE
  EAEAETATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEIT
  IGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETL
  HDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001635
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001635.1, NM_001635.2, NM_001635.3, NP_001626.1
• RefSeq Size: 3426 bp
• RefSeq ORF: 2088 bp
• Locus ID: 273
• Cytogenetics: 7p14.1
• Domains: SH3, BAR, BAR
• Protein Families: Secreted Protein
• Protein Pathways: Fc gamma R-mediated phagocytosis
• MW: 76.2 kDa
• Gene Summary: 'This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]'