ANKMY2 (NM_020319) Human Tagged ORF Clone

CAT#: RC206770

ANKMY2 (Myc-DDK-tagged)-Human ankyrin repeat and MYND domain containing 2 (ANKMY2)

Product Images

Western blot validation of overexpression lysate (Cat# LY412559) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206770 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ANKMY2 protein (Cat# TP306770). The protein was produced from HEK293T cells transfected with ANKMY2 cDNA clone (Cat# RC206770) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKMY2
• Synonyms: ZMYND20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206770 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCACATAAAGAAAGGCGAGCTGACCCAGGAGGAGAAGGAGCTACTGGAAGTCATCGGGAAAGGTA
  CTGTCCAAGAAGCTGGAACATTATTATCCAGCAAGAATGTTCGTGTCAACTGTTTGGACGAGAATGGAAT
  GACTCCTCTAATGCATGCAGCATATAAAGGAAAACTCGATATGTGCAAATTACTACTGCGACATGGAGCC
  GATGTAAATTGTCATCAGCATGAACATGGATACACAGCCCTCATGTTTGCTGCACTTTCTGGTAATAAAG
  ACATCACATGGGTAATGTTAGAAGCTGGTGCTGAGACAGATGTTGTCAACTCTGTGGGAAGAACAGCAGC
  TCAGATGGCAGCCTTTGTGGGTCAACATGATTGTGTGACCATAATCAACAATTTCTTTCCTCGAGAGAGA
  CTGGATTATTACACTAAGCCCCAGGGACTGGATAAAGAGCCAAAACTGCCCCCAAAGTTGGCAGGCCCGC
  TGCACAAAATTATCACCACAACGAATCTTCATCCTGTCAAGATCGTGATGCTTGTAAATGAGAATCCTCT
  GCTGACAGAAGAAGCAGCCCTGAATAAATGCTACAGAGTGATGGATTTGATTTGTGAGAAATGTATGAAG
  CAAAGAGACATGAATGAAGTATTGGCTATGAAGATGCATTACATAAGCTGTATCTTTCAGAAATGCATTA
  ACTTCTTAAAAGATGGAGAGAATAAACTGGACACCTTGATCAAAAGCTTGTTAAAAGGCCGAGCTTCTGA
  TGGCTTTCCAGTGTATCAAGAAAAGATCATTAGAGAAAGTATCAGAAAATTTCCTTACTGTGAAGCTACA
  CTCCTCCAGCAGCTGGTTCGAAGCATTGCTCCTGTTGAAATTGGTTCTGATCCCACTGCATTCTCCGTCC
  TTACCCAAGCCATCACTGGCCAGGTGGGTTTTGTGGATGTGGAATTTTGCACTACCTGTGGAGAAAAGGG
  AGCAAGTAAAAGATGTTCAGTTTGCAAAATGGTAATATATTGTGATCAAACCTGCCAGAAAACACACTGG
  TTTACTCATAAGAAAATCTGTAAGAATCTGAAGGACATTTACGAAAAGCAACAGTTGGAGGCTGCCAAAG
  AAAAGAGACAAGAGGAAAACCACGGCAAACTTGATGTCAATTCTAACTGTGTTAATGAAGAGCAACCAGA
  GGCTGAAGTAGGTATCTCTCAAAAGGATTCTAATCCTGAAGATTCCGGGGAAGGAAAGAAAGAATCTCTT
  GAAAGCGAAGCTGAGTTGGAAGGCTTACAGGATGCTCCTGCAGGGCCACAGGTGTCTGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206770 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVHIKKGELTQEEKELLEVIGKGTVQEAGTLLSSKNVRVNCLDENGMTPLMHAAYKGKLDMCKLLLRHGA
  DVNCHQHEHGYTALMFAALSGNKDITWVMLEAGAETDVVNSVGRTAAQMAAFVGQHDCVTIINNFFPRER
  LDYYTKPQGLDKEPKLPPKLAGPLHKIITTTNLHPVKIVMLVNENPLLTEEAALNKCYRVMDLICEKCMK
  QRDMNEVLAMKMHYISCIFQKCINFLKDGENKLDTLIKSLLKGRASDGFPVYQEKIIRESIRKFPYCEAT
  LLQQLVRSIAPVEIGSDPTAFSVLTQAITGQVGFVDVEFCTTCGEKGASKRCSVCKMVIYCDQTCQKTHW
  FTHKKICKNLKDIYEKQQLEAAKEKRQEENHGKLDVNSNCVNEEQPEAEVGISQKDSNPEDSGEGKKESL
  ESEAELEGLQDAPAGPQVSEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020319
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020319.1, NM_020319.2, NP_064715.1
• RefSeq Size: 2567 bp
• RefSeq ORF: 1326 bp
• Locus ID: 57037
• MW: 49.3 kDa
• Gene Summary: May be involved in the trafficking of signaling proteins to the cilia. [UniProtKB/Swiss-Prot Function]