MSL3L1 (MSL3) (NM_078629) Human Tagged ORF Clone

CAT#: RC206888

MSL3 (Myc-DDK-tagged)-Human male-specific lethal 3 homolog (Drosophila) (MSL3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409198) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206888 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MSL3 protein (Cat# TP306888). The protein was produced from HEK293T cells transfected with MSL3 cDNA clone (Cat# RC206888) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSL3
• Synonyms: MSL3L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206888 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCGAGCGAGGGCATGAAATTTAAATTCCACTCAGGGGAGAAAGTGCTGTGCTTCGAGCCTGACC
  CCACCAAGGCGCGAGTGCTGTACGATGCCAAGATTGTTGATGTTATTGTTGGGAAAGACGAAAAAGGCAG
  AAAGATCCCAGAATATCTGATCCATTTTAATGGTTGGAACAGAAGCTGGGATAGATGGGCAGCAGAAGAT
  CATGTGCTTCGTGATACCGATGAAAATCGTAGATTACAGCGTAAATTGGCAAGAAAAGCTGTAGCTCGCC
  TGAGGAGCACAGGAAGAAAGAAGAAGCGCTGCAGGTTGCCTGGTGTGGACTCTGTCTTAAAAGGCCTCCC
  CACTGAAGAAAAAGATGAAAATGATGAAAACTCATTAAGCAGTTCCTCTGACTGTAGTGAAAACAAGGAT
  GAAGAAATAAGTGAAGAAAGTGATATTGAAGAAAAGACTGAAGTGAAAGAAGAACCAGAGCTTCAAACAA
  GAAGGGAAATGGAAGAAAGAACAATAACTATAGAAATCCCTGAAGTTCTGAAGAAGCAGCTGGAGGATGA
  TTGTTACTACATTAACAGGAGGAAACGGTTAGTGAAACTTCCATGCCAGACCAACATCATAACGATTTTG
  GAATCCTATGTGAAGCATTTTGCTATCAATGCAGCCTTTTCAGCCAATGAGAGGCCTCGTCACCATCACG
  TTATGCCACATGCCAACATGAACGTGCATTATATCCCAGCAGAAAAGAATGTTGACCTTTGTAAGGAGAT
  GGTGGATGGATTAAGAATAACCTTTGATTACACTCTCCCGTTGGTTTTACTCTATCCATATGAACAAGCT
  CAGTATAAAAAGGTGACTTCGTCTAAATTTTTTCTTCCAATTAAGGAAAGTGCCACAAGCACTAACAGGA
  GCCAGGAGGAACTCTCTCCCAGTCCGCCTTTGTTGAATCCATCCACGCCACAGTCCACAGAGAGTCAGCC
  GACCACCGGTGAACCAGCCACCCCCAAAAGGCGCAAAGCTGAGCCAGAAGCATTGCAGTCTCTGAGGCGG
  TCCACGCGCCACAGTGCCAACTGTGACAGGCTTTCTGAGAGCAGCGCTTCACCTCAGCCCAAGCGCCGGC
  AGCAGGACACATCCGCCAGCATGCCCAAGCTCTTCCTGCACCTGGAAAAGAAGACACCTGTGCATAGCAG
  ATCATCTTCACCTATTCCTCTGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAGGGAGA
  AGAACTAATGAAATAAACGAGGTCCTCTCCTGGAAGCTTGTGCCTGACAATTACCCCCCAGGTGACCAGC
  CGCCTCCACCCTCTTACATTTATGGGGCACAACATTTGCTGCGATTGTTTGTGAAACTTCCAGAAATCCT
  TGGAAAGATGTCCTTTTCTGAGAAGAATCTGAAGGCTTTATTGAAGCACTTTGATCTCTTTTTGAGGTTT
  TTAGCAGAATACCACGATGACTTCTTCCCAGAGTCGGCTTATGTCGCTGCCTGTGAGGCACATTACAGCA
  CCAAGAACCCCCGGGCAATTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206888 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASEGMKFKFHSGEKVLCFEPDPTKARVLYDAKIVDVIVGKDEKGRKIPEYLIHFNGWNRSWDRWAAED
  HVLRDTDENRRLQRKLARKAVARLRSTGRKKKRCRLPGVDSVLKGLPTEEKDENDENSLSSSSDCSENKD
  EEISEESDIEEKTEVKEEPELQTRREMEERTITIEIPEVLKKQLEDDCYYINRRKRLVKLPCQTNIITIL
  ESYVKHFAINAAFSANERPRHHHVMPHANMNVHYIPAEKNVDLCKEMVDGLRITFDYTLPLVLLYPYEQA
  QYKKVTSSKFFLPIKESATSTNRSQEELSPSPPLLNPSTPQSTESQPTTGEPATPKRRKAEPEALQSLRR
  STRHSANCDRLSESSASPQPKRRQQDTSASMPKLFLHLEKKTPVHSRSSSPIPLTPSKEGSAVFAGFEGR
  RTNEINEVLSWKLVPDNYPPGDQPPPPSYIYGAQHLLRLFVKLPEILGKMSFSEKNLKALLKHFDLFLRF
  LAEYHDDFFPESAYVAACEAHYSTKNPRAIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_078629
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078629.1, NM_078629.2, NM_078629.3, NP_523353.1
• RefSeq Size: 2359
• RefSeq ORF: 1566
• Locus ID: 10943
• Domains: CHROMO
• Protein Families: Transcription Factors
• MW: 59.8 kDa
• Gene Summary: This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus, the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation, and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 7 and 8. [provided by RefSeq, Jul 2010]