STK33 (NM_030906) Human Tagged ORF Clone

CAT#: RC206910

STK33 (Myc-DDK-tagged)-Human serine/threonine kinase 33 (STK33)

Product Images

Western blot validation of overexpression lysate (Cat# LY403089) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206910 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified STK33 protein (Cat# TP306910). The protein was produced from HEK293T cells transfected with STK33 cDNA clone (Cat# RC206910) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK33
• Synonyms: OTTHUMP00000178934; OTTHUMP00000178935; serine/threonine kinase 33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206910 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATAGTGGCTTAGATAAAAAATCCACAAAATGCCCCGACTGTTCATCTGCTTCTCAGAAAGATG
  TACTTTGTGTATGTTCCAGCAAAACAAGGGTTCCTCCAGTTTTGGTGGTGGAAATGTCACAGACATCAAG
  CATTGGTAGTGCAGAATCTTTAATTTCACTGGAGAGAAAAAAAGAAAAAAATATCAACAGAGATATAACC
  TCCAGGAAAGATTTGCCCTCAAGAACCTCAAATGTAGAGAGAAAAGCATCTCAGCAACAATGGGGTCGGG
  GCAACTTTACAGAAGGAAAAGTTCCTCACATAAGGATTGAGAATGGAGCTGCTATTGAGGAAATCTATAC
  CTTTGGAAGAATATTGGGAAAAGGGAGCTTTGGAATAGTCATTGAAGCTACAGACAAGGAAACAGAAACG
  AAGTGGGCAATTAAAAAAGTGAACAAAGAAAAGGCTGGAAGCTCCGCTGTGAAGTTACTTGAACGAGAGG
  TGAACATTCTGAAAAGTGTAAAACATGAACACATCATACATCTGGAACAAGTATTTGAAACGCCAAAGAA
  AATGTACCTTGTGATGGAGCTTTGTGAGGATGGAGAACTCAAAGAAATTCTGGATAGGAAAGGGCATTTC
  TCAGAGAATGAGACAAGGTGGATCATTCAAAGTCTCGCATCAGCTATAGCATATCTTCACAATAATGATA
  TTGTACATAGGGATCTGAAACTGGAAAATATAATGGTTAAAAGCAGTCTTATTGATGATAACAATGAAAT
  AAACTTAAACATAAAGGTGACTGATTTTGGCTTAGCGGTGAAGAAGCAAAGTAGGAGTGAAGCCATGCTG
  CAGGCCACATGTGGGACTCCTATCTATATGGCCCCTGAAGTTATCAGTGCCCACGACTATAGCCAGCAGT
  GTGACATTTGGAGCATAGGCGTCGTAATGTACATGTTATTACGTGGAGAACCACCCTTTTTGGCAAGCTC
  AGAAGAGAAGCTTTTTGAGTTAATAAGAAAAGGAGAACTACATTTTGAAAATGCAGTCTGGAATTCCATA
  AGTGACTGTGCTAAAAGTGTTTTGAAACAACTTATGAAAGTAGATCCTGCTCACAGAATCACAGCTAAGG
  AACTACTAGATAACCAGTGGTTAACAGGCAATAAACTTTCTTCGGTGAGACCAACCAATGTATTAGAGAT
  GATGAAGGAATGGAAAAATAACCCAGAAAGTGTTGAGGAAAACACAACAGAAGAGAAGAATAAGCCGTCC
  ACTGAAGAAAAGTTGAAAAGTTACCAACCCTGGGGAAATGTCCCTGAGACCAATTACACTTCAGATGAAG
  AGGAGGAAAAACAGTCTACTACTTATGAAAAGCAATTTCCTGCAACCAGTAAGGACAACTTTGATATGTG
  CAGTTCAAGTTTCACATCTAGCAAACTCCTTCCAGCTGAAATCAAGGGAGAAATGGAGAAAACCCCTGTG
  ACTCCAAGCCAAGGAACAGCAACCAAGTACCCTGCTAAATCCGGCGCCCTGTCCAGAACCAAAAAGAAAC
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206910 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDIT
  SRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETET
  KWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHF
  SENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAML
  QATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEEKLFELIRKGELHFENAVWNSI
  SDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPS
  TEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPV
  TPSQGTATKYPAKSGALSRTKKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030906
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030906.1, NM_030906.2, NM_030906.3, NP_112168.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 1545 bp
• Locus ID: 65975
• Protein Families: Druggable Genome, Protein Kinase
• MW: 57.9 kDa