TAF1C (NM_139353) Human Tagged ORF Clone

CAT#: RC207009

TAF1C (Myc-DDK-tagged)-Human TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa (TAF1C), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF1C
• Synonyms: MGC:39976; SL1; TAFI95; TAFI110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207009 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCTCCCCTCATCGATCCCTGGGACCCTGGCCTGACTGCCCGGGACCTGCTTTTCCGCGGAGGGT
  ACCGGTATCGGAAGCGGCCCCGAGTCGTGCTGGATGTGACTGAGCAGATCAGCCGGTTCCTCTTGGATCA
  TGGAGACGTAGCCTTTGCGCCCCTGGGGAAGCTGATGCTGGAGAATTTCAAGCTGGAGGGAGCGGGGAGC
  CGCACTAAGAAGAAGACAGTGGTCAGTGTGAAGAAGCTGCTCCAGGACCTCGGTGGACACCAGCCCTGGG
  GGTGTCCCTGGGCTTACCTCAGCAACCGACAGCGCCGCTTCTCTATCCTCGGGGGCCCCATCCTGGGCAC
  GTCGGTGGCGAGCCACTTGGCAGAGCTGCTGCACGAGGAGCTGGTGCTGCGGTGGGAGCAGCTGCTTCTG
  GATGAGGCCTGCACTGGGGGCGCGCTGGCCTGGGTTCCTGGAAGGACACCCCAGTTCGGGCAGCTGGTCT
  ACCCTGCTGGAGGCGCCCAGGACAGGCTGCATTTCCAAGAGGTCGTTCTGACCCCAGGTGACAATCCCCA
  ATTCCTTGGGAAACCTGGACGCATCCAGCTCCAGGGACCTGTCCGGCAAGTGGTGACATGCACCGTCCAG
  GGAGAAACTCTGCTGGCCGTCCGCTCTGACTACCACTGTGCCGTGTGGAAGTTTGGTAAACAGTGGCAGC
  CAACCCTTCTGCAGGCGATGCAGGTGGAGAAAGGGGCCACGGGGATCAGCCTCAGCCCTCACCTGCCCGG
  GGAGCTGGCCATCTGCAGCCGCTCGGGAGCCGTCTGCCTGTGGAGCCCTGAGGATGGGCTGCGGCAAATC
  TACAGGGACCCTGAGACCCTCGTGTTCCGGGACTCCTCTTCGTGGCGTTGGGCAGACTTCACTGCGCACC
  CTCGGGTGCTGACCGTGGGTGACCGCACCGGAGTGAAGATGCTGGACACTCAGGGCCCGCCGGGCTGTGG
  TCTGTTGCTTTTTCGTTTGGGGGCAGAGGCTTCGTGCCAGAAAGGGGAACGTGTCCTGCTTACCCAGTAC
  CTGGGGCACTCCAGCCCCAAATGCCTCCCCCCTACTCTTCATCTCGTCTGTACCCAGTTCTCTCTCTACC
  TAGTGGACGAGCGCCTTCCCCTGGTGCCGATGCTGAAGTGGAACCATGGCCTCCCCTCCCCGCTCCTGCT
  GGCCCGACTGCTGCCTCCGCCCCGGCCCAGCTGCGTGCAGCCCCTGCTCCTCGGAGGCCAGGGTGGGCAG
  CTGCAGCTGCTGCACCTGGCAGAAGGGGCGTCGGTGCCCCGCCTGGCAGGCCCCCCCCAGTCTCTTCCTT
  CCAGGATCGACTCCCTCCCTGCATTTCCTCTGCTGGAGCCTAAGATCCAGTGGCGGCTGCAGGAGCGCCT
  GAAAGCACCGACCATAGGTCTGGCTGCCGTCGTCCCGCCCATGCCCTCAGCGCCCACACCAGGCCTGGTG
  CTCTTCCAGCTCTCGGCGGCGGGAGATGTCTTCTACCAGCAGCTCCGCCCCCAGGTGGACTCCAGCCTCC
  GCAGAGATGCTGGGCCTCCTGGCGACACCCAACCTGACTGCCATGCCCCCACAGCTTCCTGGACCTCCCA
  GGACACTGCCGGCTGCAGCCAGTGGCTGAAGGCCCTGCTAAAAGTGCCCCTGGCTCCTCCTGTGTGGACA
  GCACCCACCTTCACCCACCGCCAGATGCTGGGCAGCACAGAGCTGCGGAGGGAGGAAGAGGAAGGGCAGC
  GGCTGGGTGTACTCCGCAAGGCCATGGCCCGAGGGCAGCTCCTGCTGCAGAGAGACCTGGGCTCCCTCCC
  TGCGGCAGAGCCACCCCCTGCACCCGAGTCAGGCCTAGAGGACAAGCTCAGTGAGCGCCTGGGGGAAGCC
  TGGGCAGGCCGAGGGGCTGCCTGGTGGGAGAGGCAGCAGGGCAGGACCTCGGAGCCCGGGAGACAGACCA
  GGCGGCCCAAGCGCCGGACCCAGCTGTCCAGCAGCTTTTCGCTCAGTGGCCATGTGGATCCCTCAGAGGA
  CACCAGCTCCCCTCATAGCCCTGAGTGGCCACCTGCTGATGCTCTGCCCCTGCCCCCCACGACCCCGCCC
  TCCCAGGAGTTGACTCCGGATGCATGCGCCCAGGGCGTCCCATCAGAGCAGCGGCAGATGCTCCGTGACT
  ACATGGCCAAGCTACCACCCCAGAGGGACACCCCAGGCTGTGCCACCACACCTCCCCACTCCCAGGCCTC
  CAGCGTCCGGGCCACTCGCTCCCAGCAGCACACACCCGTCCTCTCTAGCTCTCAGCCCCTCCGTAAGAAG
  CCTCGAATGGGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207009 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPPLIDPWDPGLTARDLLFRGGYRYRKRPRVVLDVTEQISRFLLDHGDVAFAPLGKLMLENFKLEGAGS
  RTKKKTVVSVKKLLQDLGGHQPWGCPWAYLSNRQRRFSILGGPILGTSVASHLAELLHEELVLRWEQLLL
  DEACTGGALAWVPGRTPQFGQLVYPAGGAQDRLHFQEVVLTPGDNPQFLGKPGRIQLQGPVRQVVTCTVQ
  GETLLAVRSDYHCAVWKFGKQWQPTLLQAMQVEKGATGISLSPHLPGELAICSRSGAVCLWSPEDGLRQI
  YRDPETLVFRDSSSWRWADFTAHPRVLTVGDRTGVKMLDTQGPPGCGLLLFRLGAEASCQKGERVLLTQY
  LGHSSPKCLPPTLHLVCTQFSLYLVDERLPLVPMLKWNHGLPSPLLLARLLPPPRPSCVQPLLLGGQGGQ
  LQLLHLAEGASVPRLAGPPQSLPSRIDSLPAFPLLEPKIQWRLQERLKAPTIGLAAVVPPMPSAPTPGLV
  LFQLSAAGDVFYQQLRPQVDSSLRRDAGPPGDTQPDCHAPTASWTSQDTAGCSQWLKALLKVPLAPPVWT
  APTFTHRQMLGSTELRREEEEGQRLGVLRKAMARGQLLLQRDLGSLPAAEPPPAPESGLEDKLSERLGEA
  WAGRGAAWWERQQGRTSEPGRQTRRPKRRTQLSSSFSLSGHVDPSEDTSSPHSPEWPPADALPLPPTTPP
  SQELTPDACAQGVPSEQRQMLRDYMAKLPPQRDTPGCATTPPHSQASSVRATRSQQHTPVLSSSQPLRKK
  PRMGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139353
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139353.1, NM_139353.2, NP_647610.1
• RefSeq Size: 3720
• RefSeq ORF: 2328
• Locus ID: 9013
• Protein Families: Transcription Factors
• MW: 85.1 kDa
• Gene Summary: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011]