p107 (RBL1) (NM_183404) Human Tagged ORF Clone
CAT#: RC207017L2
- LentiORF®
Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, mGFP tagged
"NM_183404" in other vectors (6)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | RBL1 |
Synonyms | CP107; p107; PRB1 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC207017).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_183404 |
ORF Size | 3042 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_183404.1, NP_899662.1 |
RefSeq Size | 3305 bp |
RefSeq ORF | 3045 bp |
Locus ID | 5933 |
Cytogenetics | 20q11.23 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Cell cycle, TGF-beta signaling pathway |
MW | 114.7 kDa |
Gene Summary | 'The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109715 | RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2 |
USD 310.00 |
|
RC207017 | RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2 |
USD 1,060.00 |
|
RG207017 | RBL1 (GFP-tagged) - Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2 |
USD 1,170.00 |
|
RC207017L1 | Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, Myc-DDK-tagged |
USD 1,560.00 |
|
RC207017L3 | Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, Myc-DDK-tagged |
USD 1,260.00 |
|
RC207017L4 | Lenti ORF clone of Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2, mGFP tagged |
USD 1,260.00 |
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