p107 (RBL1) (NM_183404) Human Untagged Clone

CAT#: SC109715

RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2


  "NM_183404" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RBL1
Synonyms CP107; p107; PRB1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_183404, the custom clone sequence may differ by one or more nucleotides


ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCGCTACAGGCCC
TGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGACGACTTTACTGCCATCCGAGG
CAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCATGTTCATTATATGTTGCATGCCGCAAAAGC
ATTATTCCCACGGTTGGAAAGGGTATCATGGAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAG
CTAAATTAAGTTTAATACAATTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGA
ATTTCGTGAACGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGCCGGAAGCAGA
GGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTTTTTGTTTATACTAAGGGTAA
TTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCATTTACTTCTATGCTGCTTGGATCTGATTTTT
GCCAATGCGATTATGTGCCCAAATAGACAAGACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATT
TTCATACTGCTGACTTTACGGCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGA
TGGACTTCTCGTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAAGCAGTGAATA
AGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATCTTTTTGGGAGCAGACGCAGA
AGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACCCCATTAGGGAAACTGACAGCACAGGCTAAT
GTGGAGTATAACCTTCAACAGCACTTTGAAAAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGAC
GGAGATATTTACGAGAAAAAGAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTT
ACAGAGTATTGTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAACACTATACTC
AATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGACTAAAGCTGGCAGAAATTTT
GTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACACGAAGACTTCATGGAATGGACATGTCAGTT
CTTTTAGAGCAAGATATATTTCATCGTTCCTTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATA
GCTCACCTCGTACTTTTCCTTGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTAT
TGAGGTGGTGATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTTTCTGCAAACA
AAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGAAATGGAGGAAATGTGCAGGG
ACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCAAGAGTCAAGGAAGTTCGAACTGACAGTGGG
AGTCTTCGAAGAGATATGCAACCATTGTCTCCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAG
GGAGTGCTAAGAGAAGACTCTTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGA
AGGAACAAAATTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATTCCATTACATG
GTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAATACAAATCAGGAGTCCAAAGT
CAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGTGCTTCTCCAAAACAGACCAATCTGACTAAA
GCACAAGAGGTACATTCAACTGGAATAAACAGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAA
AGGTCTATCATTTGGCAAGTGTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACG
AAGGAAGATATGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACTTTTCAAGAAA
TTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGAAGTGTTCTGCTGAAAAGTAT
TCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGATGACTTTGAAATGATAGATTGTGACTTAGAA
GATGCTACAAAAACACCTGACTGTTCCAGTGGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTT
ACAATACAATATATGTAGGAAGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATAT
GATGGATGCTCCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGCGCTCTGCTGT
ACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTGA


>OriGene 5' read for NM_183404 unedited
TAATACCGACTTCACCTATNAGTGGANCGGCACCGCGNAATTNCGGCCACGAAGGGCGCC
CTGGGNAGAGGAGAAAGAAGTCGGGGGCCGTGGCGCGCAGCCCGCGGGGCCTGAAGGGAT
GTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCGCT
ACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGACGA
CTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCATG
TTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATGGA
AGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAATT
TTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAACG
TATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAGCC
AATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGCCG
GAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTNTN
TGTTTATACTAAGGGTAATTNTCGGATGATTGGGGATGACTTANTAAACTCTTATCATTT
ACTTCTATGCTGCTAGGATCTGATNTTTGCCAATGCGATTATGTGCCCAAATANGACAGA
CTTTGCTAACCATCATTTAAGGGTTACATCTGATNTTATACTGCTGACTTACGGCTNNCT
GAGAGCACCTGCATCATTGCTGTACTGGGNGACTGCATGATGGACTNCTCGTAAAGCAAA
GGGATAAGGAGCCCTCTCTAGCCCTTATTCAAACTCTTGCAGGAGATTAAAGGAAATGCC
TCTGACCTTNCAGTTACGTATAGCAAC
>Forward primer walk for NM_183404 unedited
CTCCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTT
CCCAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAA
GCGCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTGAATAGGCTAAAAGT
TGGATTTCAGATTAACAGTCAGTAACTGTTAATCCTGCCTCTTTTTTTTTTTTTCTTTTT
TGGCCAAGTTACTACTATACTAATAAAATATGAAATGTTAAAAAAAAAAAAAAAAAACTC
GACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCT
GTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCC
TTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTA
TGGG
Restriction Sites NotI-NotI     
ACCN NM_183404
Insert Size 2950 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_183404.1, NP_899662.1
RefSeq Size 3371 bp
RefSeq ORF 3045 bp
Locus ID 5933
Cytogenetics 20q11.23
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Cell cycle, TGF-beta signaling pathway
Gene Summary 'The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.

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