NALP12 (NLRP12) (NM_144687) Human Tagged ORF Clone
CAT#: RC207045
NLRP12 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2
"NM_144687" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NLRP12 |
Synonyms | CLR19.3; FCAS2; NALP12; PAN6; PYPAF7; RNO; RNO2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207045 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACGAACCGCAGGCAGGGACGGCCTCTGTCGCCTGTCCACCTACTTGGAAGAACTCGAGGCTGTGG AACTGAAGAAGTTCAAGTTATACCTGGGGACCGCGACAGAGCTGGGAGAAGGCAAGATCCCCTGGGGAAG CATGGAGAAGGCCGGTCCCCTGGAAATGGCCCAGCTGCTCATCACCCACTTCGGGCCAGAGGAGGCCTGG AGGTTGGCTCTCAGCACCTTTGAGCGGATAAACAGGAAGGACCTGTGGGAGAGAGGACAGAGAGAGGACC TGGTGAGGGATACCCCACCTGGTGGCCCGTCCTCACTTGGGAACCAGTCAACATGCCTTCTGGAAGTCTC TCTTGTCACTCCAAGAAAAGATCCCCAGGAAACCTACAGGGACTATGTCCGCAGGAAATTCCGGCTCATG GAAGACCGCAATGCGCGCCTAGGGGAATGTGTCAACCTCAGCCACCGGTACACCCGGCTCCTGCTGGTGA AGGAGCACTCAAACCCCATGCAGGTCCAGCAGCAGCTTCTGGACACAGGCCGGGGACACGCGAGGACCGT GGGACACCAGGCTAGCCCCATCAAGATAGAGACCCTCTTTGAGCCAGACGAGGAGCGCCCCGAGCCACCG CGCACCGTGGTCATGCAAGGCGCGGCAGGGATAGGCAAGTCCATGCTGGCACACAAGGTGATGCTGGACT GGGCGGACGGGAAGCTCTTCCAAGGCAGATTTGATTATCTCTTCTACATCAACTGCAGGGAGATGAACCA GAGTGCCACGGAATGCAGCATGCAAGACCTCATCTTCAGCTGCTGGCCTGAGCCCAGCGCGCCTCTCCAG GAGCTCATCCGAGTTCCCGAGCGCCTCCTTTTCATCATCGACGGCTTCGATGAGCTCAAGCCTTCTTTCC ACGATCCTCAGGGACCCTGGTGCCTCTGCTGGGAGGAGAAACGGCCCACGGAGCTGCTTCTTAACAGCTT AATTCGGAAGAAGCTGCTCCCTGAGCTATCTTTGCTCATCACCACACGGCCCACGGCTTTGGAGAAGCTC CACCGTCTGCTGGAGCACCCCAGGCATGTGGAGATCCTGGGCTTCTCTGAGGCAGAAAGGAAGGAATACT TCTACAAGTATTTCCACAATGCAGAGCAGGCGGGCCAAGTCTTCAATTACGTGAGGGACAACGAGCCTCT CTTCACCATGTGCTTCGTCCCCCTGGTGTGCTGGGTGGTGTGTACCTGCCTCCAGCAGCAGCTGGAGGGT GGGGGGCTGTTGAGACAGACGTCCAGGACCACCACTGCAGTGTACATGCTCTACCTGCTGAGTCTGATGC AACCCAAGCCGGGGGCCCCGCGCCTCCAGCCCCCACCCAACCAGAGAGGGTTGTGCTCCTTGGCGGCAGA TGGGCTCTGGAATCAGAAAATCCTATTTGAGGAGCAGGACCTCCGGAAGCACGGCCTAGACGGGGAAGAC GTCTCTGCCTTCCTCAACATGAACATCTTCCAGAAGGACATCAACTGTGAGAGGTACTACAGCTTCATCC ACTTGAGTTTCCAGGAATTCTTTGCAGCTATGTACTATATCCTGGACGAGGGGGAGGGCGGGGCAGGCCC AGACCAGGACGTGACCAGGCTGTTGACCGAGTACGCGTTTTCTGAAAGGAGCTTCCTGGCACTCACCAGC CGCTTCCTGTTTGGACTCCTGAACGAGGAGACCAGGAGCCACCTGGAGAAGAGTCTCTGCTGGAAGGTCT CGCCGCACATCAAGATGGACCTGTTGCAGTGGATCCAAAGCAAAGCTCAGAGCGACGGCTCCACCCTGCA GCAGGGCTCCTTGGAGTTCTTCAGCTGCTTGTACGAGATCCAGGAGGAGGAGTTTATCCAGCAGGCCCTG AGCCACTTCCAGGTGATCGTGGTCAGCAACATTGCCTCCAAGATGGAGCACATGGTCTCCTCGTTCTGTC TGAAGCGCTGCAGGAGCGCCCAGGTGCTGCACTTGTATGGCGCCACCTACAGCGCGGACGGGGAAGACCG CGCGAGGTGCTCCGCAGGAGCGCACACGCTGTTGGTGCAGCTACCAGAGAGGACCGTTCTGCTGGACGCC TACAGTGAACATCTGGCAGCGGCCCTGTGCACCAATCCAAACCTGATAGAGCTGTCTCTGTACCGAAATG CCCTGGGCAGCCGGGGGGTGAAGCTGCTCTGTCAAGGACTCAGACACCCCAACTGCAAACTTCAGAACCT GAGGCTGAAGAGGTGCCGCATCTCCAGCTCAGCCTGCGAGGACCTCTCTGCAGCTCTCATAGCCAATAAG AATTTGACAAGGATGGATCTCAGTGGCAACGGCGTTGGATTCCCAGGCATGATGCTGCTTTGCGAGGGCC TGCGGCATCCCCAGTGCAGGCTGCAGATGATTCAGTTGAGGAAGTGTCAGCTGGAGTCCGGGGCTTGTCA GGAGATGGCTTCTGTGCTCGGCACCAACCCACATCTGGTTGAGTTGGACCTGACAGGAAATGCACTGGAG GATTTGGGCCTGAGGTTACTATGCCAGGGACTGAGGCACCCAGTCTGCAGACTACGGACTTTGTGGCTGA AGATCTGCCGCCTCACTGCTGCTGCCTGTGACGAGCTGGCCTCAACTCTCAGTGTGAACCAGAGCCTGAG AGAGCTGGACCTGAGCCTGAATGAGCTGGGGGACCTCGGGGTGCTGCTGCTGTGTGAGGGCCTCAGGCAT CCCACGTGCAAGCTCCAGACCCTGCGGTTGGGCATCTGCCGGCTGGGCTCTGCCGCCTGTGAGGGTCTTT CTGTGGTGCTCCAGGCCAACCACAACCTCCGGGAGCTGGACTTGAGTTTCAACGACCTGGGAGACTGGGG CCTGTGGTTGCTGGCTGAGGGGCTGCAACATCCCGCCTGCAGACTCCAGAAACTGTGGCTGGATAGCTGT GGCCTCACAGCCAAGGCTTGTGAGAATCTTTACTTCACCCTGGGGATCAACCAGACCTTGACCGACCTTT ACCTGACCAACAACGCCCTAGGGGACACAGGTGTCCGACTGCTTTGCAAGCGGCTGAGCCATCCTGGCTG CAAACTCCGAGTCCTCTGGTTATTTGGGATGGACCTGAATAAAATGACCCACAGTAGGTTGGCAGCGCTT CGAGTAACAAAACCTTATTTGGACATTGGCTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC207045 protein sequence
Red=Cloning site Green=Tags(s) MLRTAGRDGLCRLSTYLEELEAVELKKFKLYLGTATELGEGKIPWGSMEKAGPLEMAQLLITHFGPEEAW RLALSTFERINRKDLWERGQREDLVRDTPPGGPSSLGNQSTCLLEVSLVTPRKDPQETYRDYVRRKFRLM EDRNARLGECVNLSHRYTRLLLVKEHSNPMQVQQQLLDTGRGHARTVGHQASPIKIETLFEPDEERPEPP RTVVMQGAAGIGKSMLAHKVMLDWADGKLFQGRFDYLFYINCREMNQSATECSMQDLIFSCWPEPSAPLQ ELIRVPERLLFIIDGFDELKPSFHDPQGPWCLCWEEKRPTELLLNSLIRKKLLPELSLLITTRPTALEKL HRLLEHPRHVEILGFSEAERKEYFYKYFHNAEQAGQVFNYVRDNEPLFTMCFVPLVCWVVCTCLQQQLEG GGLLRQTSRTTTAVYMLYLLSLMQPKPGAPRLQPPPNQRGLCSLAADGLWNQKILFEEQDLRKHGLDGED VSAFLNMNIFQKDINCERYYSFIHLSFQEFFAAMYYILDEGEGGAGPDQDVTRLLTEYAFSERSFLALTS RFLFGLLNEETRSHLEKSLCWKVSPHIKMDLLQWIQSKAQSDGSTLQQGSLEFFSCLYEIQEEEFIQQAL SHFQVIVVSNIASKMEHMVSSFCLKRCRSAQVLHLYGATYSADGEDRARCSAGAHTLLVQLPERTVLLDA YSEHLAAALCTNPNLIELSLYRNALGSRGVKLLCQGLRHPNCKLQNLRLKRCRISSSACEDLSAALIANK NLTRMDLSGNGVGFPGMMLLCEGLRHPQCRLQMIQLRKCQLESGACQEMASVLGTNPHLVELDLTGNALE DLGLRLLCQGLRHPVCRLRTLWLKICRLTAAACDELASTLSVNQSLRELDLSLNELGDLGVLLLCEGLRH PTCKLQTLRLGICRLGSAACEGLSVVLQANHNLRELDLSFNDLGDWGLWLLAEGLQHPACRLQKLWLDSC GLTAKACENLYFTLGINQTLTDLYLTNNALGDTGVRLLCKRLSHPGCKLRVLWLFGMDLNKMTHSRLAAL RVTKPYLDIGC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144687 |
ORF Size | 3183 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144687.1, NM_144687.2, NM_144687.3, NP_653288.1 |
RefSeq Size | 3880 bp |
RefSeq ORF | 3186 bp |
Locus ID | 91662 |
Domains | LRR, PAAD_DAPIN |
MW | 120.2 kDa |
Gene Summary | This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC124283 | NLRP12 (untagged)-Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2 |
USD 310.00 |
|
RG207045 | NLRP12 (GFP-tagged) - Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2 |
USD 1,220.00 |
|
RC207045L1 | Lenti ORF clone of Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2, Myc-DDK-tagged |
USD 1,620.00 |
|
RC207045L2 | Lenti ORF clone of Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2, mGFP tagged |
USD 1,310.00 |
|
RC207045L3 | Lenti ORF clone of Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2, Myc-DDK-tagged |
USD 1,310.00 |
|
RC207045L4 | Lenti ORF clone of Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2, mGFP tagged |
USD 1,310.00 |
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