NMUR1 (NM_006056) Human Tagged ORF Clone

CAT#: RC207201

NMUR1 (Myc-DDK-tagged)-Human neuromedin U receptor 1 (NMUR1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416889) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207201 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NMUR1
• Synonyms: (FM-3); FM-3; FM3; GPC-R; GPR66; NMU1R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207201 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCCTCTCTGCCTCAATTGCTCTGTCCTCCCTGGAGACCTGTACCCAGGGGGTGCAAGGAACCCCA
  TGGCTTGCAATGGCAGTGCGGCCAGGGGGCACTTTGACCCTGAGGACTTGAACCTGACTGACGAGGCACT
  GAGACTCAAGTACCTGGGGCCCCAGCAGACAGAGCTGTTCATGCCCATCTGTGCCACATACCTGCTGATC
  TTCGTGGTGGGCGCTGTGGGCAATGGGCTGACCTGTCTGGTCATCCTGCGCCACAAGGCCATGCGCACGC
  CTACCAACTACTACCTCTTCAGCCTGGCCGTGTCGGACCTGCTGGTGCTGCTGGTGGGCCTGCCCCTGGA
  GCTCTATGAGATGTGGCACAACTACCCCTTCCTGCTGGGCGTTGGTGGCTGCTATTTCCGCACGCTACTG
  TTTGAGATGGTCTGCCTGGCCTCAGTGCTCAACGTCACTGCCCTGAGCGTGGAACGCTATGTGGCCGTGG
  TGCACCCACTCCAGGCCAGGTCCATGGTGACGCGGGCCCATGTGCGCCGAGTGCTTGGGGCCGTCTGGGG
  TCTTGCCATGCTCTGCTCCCTGCCCAACACCAGCCTGCACGGCATCCAGCAGCTGCACGTGCCCTGCCGG
  GGCCCAGTGCCAGACTCAGCTGTTTGCATGCTGGTCCGCCCACGGGCCCTCTACAACATGGTAGTGCAGA
  CCACCGCGCTGCTCTTCTTCTGCCTGCCCATGGCCATCATGAGCGTGCTCTACCTGCTCATTGGGCTGCG
  ACTGCGGCGGGAGAGGCTGCTGCTCATGCAGGAGGCCAAGGGCAGGGGCTCTGCAGCAGCCAGGTCCAGA
  TACACCTGCAGGCTCCAGCAGCACGATCGGGGCCGGAGACAAGTGACCAAGATGCTGTTTGTCCTGGTCG
  TGGTGTTTGGCATCTGCTGGGCCCCGTTCCACGCCGACCGCGTCATGTGGAGCGTCGTGTCACAGTGGAC
  AGATGGCCTGCACCTGGCCTTCCAGCACGTGCACGTCATCTCCGGCATCTTCTTCTACCTGGGCTCGGCG
  GCCAACCCCGTGCTCTATAGCCTCATGTCCAGCCGCTTCCGAGAGACCTTCCAGGAGGCCCTGTGCCTCG
  GGGCCTGCTGCCATCGCCTCAGACCCCGCCACAGCTCCCACAGCCTCAGCAGGATGACCACAGGCAGCAC
  CCTGTGTGATGTGGGCTCCCTGGGCAGCTGGGTCCACCCCCTGGCTGGGAACGATGGCCCAGAGGCGCAG
  CAAGAGACCGATCCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207201 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPLCLNCSVLPGDLYPGGARNPMACNGSAARGHFDPEDLNLTDEALRLKYLGPQQTELFMPICATYLLI
  FVVGAVGNGLTCLVILRHKAMRTPTNYYLFSLAVSDLLVLLVGLPLELYEMWHNYPFLLGVGGCYFRTLL
  FEMVCLASVLNVTALSVERYVAVVHPLQARSMVTRAHVRRVLGAVWGLAMLCSLPNTSLHGIQQLHVPCR
  GPVPDSAVCMLVRPRALYNMVVQTTALLFFCLPMAIMSVLYLLIGLRLRRERLLLMQEAKGRGSAAARSR
  YTCRLQQHDRGRRQVTKMLFVLVVVFGICWAPFHADRVMWSVVSQWTDGLHLAFQHVHVISGIFFYLGSA
  ANPVLYSLMSSRFRETFQEALCLGACCHRLRPRHSSHSLSRMTTGSTLCDVGSLGSWVHPLAGNDGPEAQ
  QETDPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006056
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006056.2, NM_006056.3, NM_006056.4, NP_006047.2
• RefSeq Size: 3274 bp
• RefSeq ORF: 1281 bp
• Locus ID: 10316
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 47.3 kDa