ZNF539 (ZNF254) (NM_203282) Human Tagged ORF Clone

CAT#: RC207207

ZNF254 (Myc-DDK-tagged)-Human zinc finger protein 254 (ZNF254). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF254
• Synonyms: BMZF-5; HD-ZNF1; ZNF91L; ZNF539
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207207 ORF sequence, codon optimized.
  Due to the complexity of NM_203282, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGTCCACCACGATCCCTGGAAATGGGGCTTCTCACGTTCCGGGACGTAGCCATTGAGTTTAGCC
  TCGAGGAATGGCAGCATCTGGACATAGCCCAGCAGAACCTGTACCGCAACGTGATGCTTGAGAACTACCG
  AAATTTGGCCTTTCTGGGGATCGCCGTGAGCAAACCTGATCTGATCACGTGTCTTGAGCAGGGAAAAGAA
  CCGTGGAATATGAAGAGACATGAAATGGTGGACGAGCCACCGGGCATGTGCCCACACTTTGCCCAGGATC
  TTTGGCCTGAGCAGGGCATGGAAGATAGCTTTCAAAAGGCAATCCTGCGCAGATACGGGAAGTACGGTCA
  CGAGAATCTGCAGCTGCGCAAGGGATGTAAAAGTGTCGACGAATACAAAGTCAACAAAGAAGGATATAAT
  GGTCTTAACCAGTGTTTCACAACGGCCCAGAGCAAGGTGTTCCAGTGCGATAAATATCTCAAGGTGTTCT
  ACAAATTTCTTAATTCCAACAGACCAAAGATCCGGCATACCGAGAAAAAGTCTTTTAAGTGCAAAAAGCG
  GGTCAAGCTTTTTTGCATGCTGTCTCATAAGACCCAGCACAAAAGTATATACCACCGGGAAAAATCTTAT
  AAGTGCAAGGAGTGTGGGAAAACTTTTAACTGGAGTTCAACGTTGACGAATCACAGGAAAATCTATACTG
  AGGAAAAGCCTTACAAATGCGAGGAGTATAACAAGTCTCCAAAGCAGTTGTCAACCCTTACTACACATGA
  GATTATCCACGCCGGCGAAAAGCTGTACAAATGTGAAGAATGCGGAGAAGCTTTCAATAGGTCATCAAAC
  CTCACCACACATAAAATTATCCACACCGGCGAAAAGCCTTACAAGTGCGAGGAGTGCGGAAAGGCATTTA
  TCTGGAGTAGCACGCTTACAGAACATAAAAAGATCCACACTCGAAAGAAACCATATAAGTGCGAAGAGTG
  CGGTAAGGCATTTATCTGGTCTTCCACACTCACTAGGCATAAAAGAATGCACACCGGGGAAAAACCATAT
  AAGTGTGAAGAATGCGGAAAGGCTTTCTCACAGTCCAGCACACTGACCACTCACAAGATAATTCACACCG
  GGGAGAAACGCTACAAATGCCTCGAGTGCGGAAAGGCTTTTAAACAGCTGTCAACCCTCACAACCCATAA
  GATTATACACGTCGGGGAAAAGCTCTACAAGTGCGAGGAGTGCGGGAAGGGATTCAATCGGAGCAGCAAC
  CTGACAACCCATAAAATAATTCACACAGGAGAAAAGCCGTACAAGTGCGAGGAGTGTGGTAAAGCATTTA
  TCTGGAGCAGCACACTGACTAAACACAAGAGAATTCACACGAGGGAAAAGCCCTACAAGTGTGAAGAGTG
  TGGGAAGGCATTTATCTGGTCTAGCACCCTTACTCGACACAAGAGAATGCATACAGGAGAGAAGCCCTAT
  AAGTGCGAGGAATGCGGCAAGTCTTTTTCCCAGTCCTCTACTCTCACTACCCATAAGATAATCCACACTG
  GTGAGAAGCCTTATAAATGCGAAGAATGCGGGAAGGCATTTAACTGGAGCTCTACTCTCACTAAACACAA
  AATCATCCATACCGAGGAGAAACCATATAAATGCGAAAAATGTGGTAAGGCATTTAAACAGTCCAGCATA
  CTCACCAATCACAAACGGATTCACACCGGGGAGAAGCCCTACAAATGCGAGGAATGCGGCAAGTCCTTTA
  ACAGGTCATCTACTTTTACCAAGCACAAAGTTATCCATACTGGCGTAAAACCTTACAAGTGCGAGGAGTG
  TGGCAAGGCATTCTTTTGGTCTAGCACACTGACAAAGCACAAAAGAATACATACCGGGGAACAACCTTAC
  AAATGGGAAAAATTTGGCAAGGCGTTCAACCGCAGCAGTCATCTGACTACTGACAAGATCACTCACTGGC
  GCGAAATTCTTCAAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207207 representing NM_203282
  Red=Cloning site Green=Tags(s)
  
  MPGPPRSLEMGLLTFRDVAIEFSLEEWQHLDIAQQNLYRNVMLENYRNLAFLGIAVSKPDLITCLEQGKE
  PWNMKRHEMVDEPPGMCPHFAQDLWPEQGMEDSFQKAILRRYGKYGHENLQLRKGCKSVDEYKVNKEGYN
  GLNQCFTTAQSKVFQCDKYLKVFYKFLNSNRPKIRHTEKKSFKCKKRVKLFCMLSHKTQHKSIYHREKSY
  KCKECGKTFNWSSTLTNHRKIYTEEKPYKCEEYNKSPKQLSTLTTHEIIHAGEKLYKCEECGEAFNRSSN
  LTTHKIIHTGEKPYKCEECGKAFIWSSTLTEHKKIHTRKKPYKCEECGKAFIWSSTLTRHKRMHTGEKPY
  KCEECGKAFSQSSTLTTHKIIHTGEKRYKCLECGKAFKQLSTLTTHKIIHVGEKLYKCEECGKGFNRSSN
  LTTHKIIHTGEKPYKCEECGKAFIWSSTLTKHKRIHTREKPYKCEECGKAFIWSSTLTRHKRMHTGEKPY
  KCEECGKSFSQSSTLTTHKIIHTGEKPYKCEECGKAFNWSSTLTKHKIIHTEEKPYKCEKCGKAFKQSSI
  LTNHKRIHTGEKPYKCEECGKSFNRSSTFTKHKVIHTGVKPYKCEECGKAFFWSSTLTKHKRIHTGEQPY
  KWEKFGKAFNRSSHLTTDKITHWREILQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203282
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_203282.1, NM_203282.2, NM_203282.3, NP_975011.2, NP_975011.3
• RefSeq Size: 4127
• RefSeq ORF: 1980
• Locus ID: 9534
• Protein Families: Transcription Factors
• MW: 77.2 kDa
• Gene Summary: Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See ZFP93 (MIM 604749) for additional information on zinc finger proteins. [supplied by OMIM, Jul 2002]