DAP5 (EIF4G2) (NM_001418) Human Tagged ORF Clone

CAT#: RC207227

EIF4G2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400548) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207227 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF4G2
• Synonyms: AAG1; DAP5; NAT1; P97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207227 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG
  GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC
  TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAACGAA
  AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG
  ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT
  GATTGTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA
  GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACCACATTCA
  GACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGAACTAGAAATGTTGATGTCTATGATAA
  GCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAAC
  ATCAAATTCATTGGAGAGCTTGGCAAGCTTGATCTTATTCACGAATCTATCCTTCATAAGTGCATCAAAA
  CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT
  AATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCGA
  ATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAAGGATTCGTTTCCTGCTGCAGGATACCGTAGAGT
  TGCGAGAACACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATTCG
  TCAAGATGCAGTAAAAGATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTC
  TTTCTGGAGGGACCGTTCATGCCACCCAGGATGAAAATGGATAGGGACCCACTTGGAGGACTTGCTGATA
  TGTTTGGACAAATGCCAGGTAGCGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCAC
  CATGGGACGTCATCGTTCAAATCAACTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACACAATCG
  CAGTTTGGAGAGATGGGAGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCATAACCAGAGTC
  AGGGACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGGTTTTCTAAGAAAGGACAGCT
  TAATGCAGATGAGATTAGCCTGAGGCCTGCTCAGTCGTTCCTAATGAATAAAAATCAAGTGCCAAAGCTT
  CAGCCCCAGATAACTATGATTCCTCCTAGTGCACAACCACCACGCACTCAAACACCACCTCTGGGACAGA
  CACCTCAGCTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAGCCTGCCAAGACCAACAAAAAGCC
  ACCACCGTCAAAGGAAGAACTCCTTAAACTAACTGAAACTGTTGTGACTGAATATCTAAATAGTGGAAAT
  GCAAATGAGGCTGTCAATGGTGTAAGAGAAATGAGGGCTCCTAAACACTTTCTTCCTGAGATGTTAAGCA
  AAGTAATCATCCTGTCACTAGATAGAAGCGATGAAGATAAAGAAAAAGCAAGTTCTTTGATCAGTTTACT
  CAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAGGCTTTCCTGAATGTATTGGACCAGTGTCCC
  AAACTGGAGGTTGACATCCCTTTGGTGAAATCCTATTTAGCACAGTTTGCAGCTCGTGCCATCATTTCAG
  AGCTGGTGAGCATTTCAGAACTAGCTCAACCACTAGAAAGTGGCACCCATTTTCCTCTCTTCCTACTTTG
  TCTTCAGCAGTTAGCTAAATTACAAGATCGAGAATGGTTAACAGAACTTTTTCAACAAAGCAAGGTCAAT
  ATGCAGAAAATGCTCCCAGAAATTGATCAGAATAAGGACCGCATGTTGGAGATTTTGGAAGGAAAGGGAC
  TGAGTTTCTTATTCCCACTCCTCAAATTGGAGAAGGAACTGTTGAAGCAAATAAAGTTGGATCCATCCCC
  TCAAACCATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTCATGTAGATAAAGGATTTGTGAAC
  ATCTTAATGACTAGCTTCTTACAGTACATTTCTAGTGAAGTAAACCCCCCCAGCGATGAAACAGATTCAT
  CCTCTGCTCCTTCCAAAGAACAGTTAGAGCAGGAAAAACAACTACTACTATCTTTCAAGCCAGTAATGCA
  GAAATTTCTTCATGATCACGTTGATCTACAAGTCAGTGCCCTGTATGCTCTCCAGGTGCACTGCTATAAC
  AGCAACTTCCCAAAAGGCATGTTACTTCGCTTTTTTGTGCACTTCTATGACATGGAAATTATTGAAGAAG
  AAGCTTTCTTGGCTTGGAAAGAAGATATAACCCAAGAGTTTCCGGGAAAAGGCAAGGCTTTGTTCCAGGT
  GAATCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAAGAATCAGAGGAAGAAGCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207227 protein sequence
  Red=Cloning site Green=Tags(s)
  
  VESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANE
  KERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLA
  EDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGN
  IKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFAR
  MCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGMRSDF
  FLEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQS
  QFGEMGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKL
  QPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTNKKPPPSKEELLKLTETVVTEYLNSGN
  ANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCP
  KLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVN
  MQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVN
  ILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYN
  SNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001418
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001418.1, NM_001418.2, NM_001418.3, NP_001409.1, NP_001409.3
• RefSeq Size: 3911 bp
• RefSeq ORF: 2724 bp
• Locus ID: 1982
• Cytogenetics: 11p15.4
• Domains: eIF5C, MA3
• Protein Families: Transcription Factors
• Protein Pathways: Viral myocarditis
• MW: 102.4 kDa
• Gene Summary: 'Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]'