DAP5 (EIF4G2) (NM_001418) Human Untagged Clone

CAT#: SC119270

EIF4G2 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4G2
• Synonyms: AAG1; DAP5; NAT1; P97
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119270 sequence for NM_001418 edited (data generated by NextGen Sequencing)
  GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGA
  GGAAGTAGGGGTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGG
  AAAACCCCAGGGCAAAACGCTCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGAC
  AACTCCGCAGCAAACAACTCCGCAAACGAAAAAGAACGACATGATGCAATCTTCAGGAAA
  GTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTGACAAGCTATGCCTTGAGCTC
  CTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCTGATTGTGGAC
  AAAGCCCTANNAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA
  GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGC
  ACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGAACTAGA
  AATGTTGATGTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAGA
  GCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTT
  GATCTTATTCACGAATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAG
  AGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGATAATGAGGACA
  GTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCGA
  ATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAAGGATTCGTTTCCTGCTGCAGGAT
  ACCGTAGAGTTGCGAGAACACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCA
  AAGACGATCAATCAAATTCGTCAAGATGNNNTAAAAGATCTAGGGGTGTTTATTCCTGCT
  CCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGGAGGGACCGTTCATGCCACCCAGG
  ATGAAAATGGATAGGGACCCACTTGGAGGACTTGCTGATATGTTTGGACAAATGCCAGGT
  AGCGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGT
  CATCGTTCAAATCAACTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACACAATCG
  CAGTTTGGAGAGATGGGAGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCAT
  AACCAGAGTCAGGGACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGG
  TTTTCTAAGAAAGGACAGCTTAATGCAGATGAGATTAGCCTGAGGCCTGCTCAGTCGTTC
  CTAATGAATAAAAATCAAGTGCCAAAGCTTCAGCCCCAGATAACTATGATTCCTCCTAGT
  GCACAACCACCACGCACTCAAACACCACCTCTGGGACAGACACCTCAGCTTGGTCTCAAA
  ACTAATCCACCACTTATCCAGGAAAAGCCTGCCAAGACCAGCAAAAAGCCACCACCGTCA
  AAGGAAGAACTCCTTAAACTAACTGAAACTGTTGTGACTGAATATCTAAATAGTGGAAAT
  GCAAATGAGGCTGTCAATGGTGTAAGAGAAATGAGGGCTCCTAAACACTTTCTTCCTGAG
  ATGTTAAGCAAAGTAATCATCCTGTCACTAGATAGAAGCGATGAAGATAAAGAAAAAGCA
  AGTTCTTTGATCAGTTTACTCAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAG
  GCTTTCCTGAATGTATTGGACCAGTGTCCCAAACTGGAGGTTGACATCCCTTTGGTGAAA
  TCCTATTTAGCACAGTTTGCAGCTCGTGCCATCATTTCAGAGCTGGTGAGCATTTCAGAA
  CTAGCTCAACCACTAGAAAGTGGCACCCATTTTCCTCTCTTCCTACTTTGTCTTCAGCAG
  TTAGCTAAATTACAAGATCGAGAATGGTTAACAGAACTTTTTCAACAAAGCAAGGTCAAT
  ATGCAGAAAATGCTCCCAGAAATTGATCAGAATAAGGACCGCATGTTGGAGATTTTGGAA
  GGAAAGGGACTGAGTTTCTTATTCCCACTCCTCAAATTGGAGAAGGAACTGTTGAAGCAA
  ATAAAGTTGGATCCATCCCCTCAAACCATATATAAATGGATTAAAGATAACATCTCTCCC
  AAACTTCATGTAGATAAAGGATTTGTGAACATCTTAATGACTAGCTTCTTACAGTACATT
  TCTAGTGAAGTAAACCCCCCCAGCGATGAAACAGATTCATCCTCTGCTCCTTCCAAAGAA
  CAGTTAGAGCAGGAAAAACAACTACTACTATCTTTCAAGCCAGTAATGCAGAAATTTCTT
  CATGATCACGTTGATCTACAAGTCAGTGCCCTGTATGCTCTCCAGGTGCACTGCTATAAC
  AGCAACTTCCCAAAAGGCATGTTACTTCGCTTTTTTGTGCACTTCTATGACATGGAAATT
  ATTGAAGAAGAAGCTTTCTTGGCTTGGAAAGAAGATATAACCCAAGAGTTTCCGGGAAAA
  GGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAA
  GAATCAGAGGAAGAAGCTGACTAA
  
  Clone variation with respect to NM_001418.3
  370 g=>n;371 a=>n;989 c=>n;990 a=>n;991 g=>n;1572 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001418 unedited
  AATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGCCTTAGCGC
  CAGCAGTGAGTCGGAGCTCTATGGAGGTGGCAGCGGGTACCGAGTGGCGGCTGCAGCAGC
  GACTCCTCTGAGCTGAGTTTGAGGCCGTCCCCGACTCCTTCCTCCCCCTTCCCTCCCCCT
  TTTTTTTGTTTTCCGTTCCCCTTTCCCCTCCCTTCCCTATCCCCGACGACCGGATCCTGA
  GGAGGCAGCTGCGGTGGCAGCTGCTGAGTTCTCGGTGAAGGTATTTCATTTCTCCTGTCC
  CCTCCCCTCCCCACCCCATCTATTAATATTATTCTTTTGAAGATTCTTCGTTGTCAAGCC
  GCCAAAGTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGC
  GGAGGAGGAAGTAGGGGTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTC
  CTGGGGAAAACCCCAGGGCAAAACGCTCAGAAATGGATTCCTGCACGAAGCACTAGACGA
  GATGACAACTCCGCAGCAAACAACTCCGCAAACGAAAAAGAACGACATGATGCAATCTTC
  AGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTGACAAGCTATGCCTT
  GAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTANAGGGGTCATACTGCTGATT
  GTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGA
  TTGGCAGAGATGCACCAAACTTTGATGCCCAGCAGCAGAGGGTCACCAGNACAGAAGCAA
  GCCCACTTCAGACGCTNCTAATTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001418 unedited
  NNNAAAAAATTAACTTGAACCGCGCCGCATACTANGATCGATNNTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTCAAAGTTTCACAATGTTTATTGATAAATACAAGTTTAT
  AAAATCAGGGCATGAACATGACTTGATAAATTAAGTAAACTTAATTTCAATACTATAATA
  GGAGGGACCAATTCAAATTCTCACCATTTGTTTCACACCCACAAAAACCACTTCAAGGGC
  ATTAACGATCTCTCAAAACTGATCAGTTTTGTGCAAGTAAACCATGTTTCTTTTAAAAAG
  ACTTGTGCACTTGCCCAGGCTCAAGTATATTAAATCTACCACATAAAGCCCATTACTAGA
  GGTAGATTACAGGCAATTACTTTTACGGCAACACCCTCAATTCAGTTAAAATTTTTTCGT
  AACAACAAATGGATAATCAAATTTTGCAACAACTCAGTTTTACTGACCAAAGGGCTTTTC
  TACAGTATTCAGGGTTGCTATTAAGTTTTCTAACTAAAAACACCTTATAAAACTGGCAAC
  AAAAAGGGCCCTTGAATAAACTGCCTTTGCTTGAAACTTATGAAGAAATTTTTGGCGGCT
  GGTAATTACCTTCTAAACTTTAAAACCCCCTAAAATTTTGGGGGGCCCCTTGGATAAAAC
  TTCCCCTCAAAAAAACCTTTGCCAAGGAAAACCTACTGGCCGGCCTTGGTTTAAAATTTG
  GAAAACTTTCCCGGAATGAATTTCCCGGGGGTTGGGAAAGGCCGTTCCTTTAACCCCCGT
  TTTTTGGCAAATTAAAGGTTGGCGGGGTTTTAAAAAACTTTTCTCTTATTTTTTTTTTAA
  AAGTTTTACACGGTTTCCCCCTTTTTCCCTGAAAAAAACTTGTCTTTTCCGAGAATTTTG
  GGTAATTTTTTTTCAAACCAAAAGCTTTTTTAAAATTCCCGGCAAAAAGGCCAAAAAAAA
  AATCACTCCTTTTGAAATTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001418
• Insert Size: 3860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001418.1, NP_001409.1
• RefSeq Size: 3820 bp
• RefSeq ORF: 2724 bp
• Locus ID: 1982
• Cytogenetics: 11p15.4
• Domains: eIF5C, MA3
• Protein Families: Transcription Factors
• Protein Pathways: Viral myocarditis
• Gene Summary: 'Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Both variants 1 and 3 encode the same isoform.