TSSK2 (NM_053006) Human Tagged ORF Clone

CAT#: RC207232

TSSK2 (Myc-DDK-tagged)-Human testis-specific serine kinase 2 (TSSK2)

Product Images

Western blot validation of overexpression lysate (Cat# LY403278) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207232 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TSSK2 protein (Cat# TP307232). The protein was produced from HEK293T cells transfected with TSSK2 cDNA clone (Cat# RC207232) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TSSK2
• Synonyms: DGS-G; SPOGA2; STK22B; TSK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207232 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGATGCCACAGTCCTAAGGAAGAAGGGTTACATCGTAGGCATCAATCTTGGCAAGGGTTCCTACG
  CAAAAGTCAAATCTGCCTACTCTGAGCGCCTCAAGTTCAATGTGGCTGTCAAGATCATCGACCGCAAGAA
  AACACCTACTGACTTTGTGGAGAGATTCCTTCCTCGGGAGATGGACATCCTGGCAACTGTCAACCACGGC
  TCCATCATCAAGACTTACGAGATCTTTGAGACCTCTGACGGACGGATCTACATCATCATGGAGCTTGGCG
  TCCAGGGCGACCTCCTCGAGTTCATCAAGTGCCAGGGAGCCCTGCATGAGGACGTGGCACGCAAGATGTT
  CCGACAGCTCTCCTCCGCCGTCAAGTACTGCCACGACCTGGACATCGTCCACCGGGACCTCAAGTGCGAG
  AACCTTCTCCTCGACAAGGACTTCAACATCAAGCTGTCTGACTTTGGCTTCTCCAAGCGCTGCCTGCGGG
  ACAGCAATGGGCGCATCATCCTCAGCAAGACCTTCTGCGGGTCGGCAGCATATGCAGCCCCCGAGGTGCT
  GCAGAGCATCCCCTACCAGCCCAAGGTGTATGACATCTGGAGCCTGGGCGTGATCCTGTACATCATGGTC
  TGCGGCTCCATGCCCTATGACGACTCCGACATCAGGAAGATGCTGCGTATCCAGAAGGAGCACCGTGTGG
  ACTTCCCGCGCTCCAAGAACCTGACCTGCGAGTGCAAGGACCTCATCTACCGCATGCTGCAGCCCGACGT
  CAGCCAGCGGCTCCACATCGATGAGATCCTCAGCCACTCGTGGCTGCAGCCCCCCAAGCCCAAAGCCACG
  TCTTCTGCCTCCTTCAAGAGGGAGGGGGAGGGCAAGTACCGCGCTGAGTGCAAACTGGACACCAAGACAG
  ACTTGAGGCCCGACCACCGGCCCGACCACAAGCTTGGAGCCAAAACCCAGCACCGGCTGCTGGTGGTGCC
  CGAGAACGAGAACAGGATGGAGGACAGGCTGGCCGAGACCTCCAGGGCCAAAGACCATCACATCTCCGGA
  GCTGAGGTGGGGAAAGCAAGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207232 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDATVLRKKGYIVGINLGKGSYAKVKSAYSERLKFNVAVKIIDRKKTPTDFVERFLPREMDILATVNHG
  SIIKTYEIFETSDGRIYIIMELGVQGDLLEFIKCQGALHEDVARKMFRQLSSAVKYCHDLDIVHRDLKCE
  NLLLDKDFNIKLSDFGFSKRCLRDSNGRIILSKTFCGSAAYAAPEVLQSIPYQPKVYDIWSLGVILYIMV
  CGSMPYDDSDIRKMLRIQKEHRVDFPRSKNLTCECKDLIYRMLQPDVSQRLHIDEILSHSWLQPPKPKAT
  SSASFKREGEGKYRAECKLDTKTDLRPDHRPDHKLGAKTQHRLLVVPENENRMEDRLAETSRAKDHHISG
  AEVGKAST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053006
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053006.1, NM_053006.2, NM_053006.3, NM_053006.4, NP_443732.2
• RefSeq Size: 1832 bp
• RefSeq ORF: 1077 bp
• Locus ID: 23617
• Protein Families: Druggable Genome, Protein Kinase
• MW: 41 kDa
• Gene Summary: TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]). [supplied by OMIM, Mar 2008]