TSSK2 (NM_053006) Human Untagged Clone

CAT#: SC323471

TSSK2 (untagged)-Kinase deficient mutant (K41M) of Human testis-specific serine kinase 2 (TSSK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSSK2
• Synonyms: DGS-G; SPOGA2; STK22B; TSK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323471 sequence for NM_053006 edited (data generated by NextGen Sequencing)
  ATGGACGATGCCACAGTCCTAAGGAAGAAGGGTTACATCGTAGGCATCAATCTTGGCAAG
  GGTTCCTACGCAAAAGTCAAATCTGCCTACTCTGAGCGCCTCAAGTTCAATGTGGCTGTC
  AAGATCATCGACCGCAAGAAAACACCTACTGACTTTGTGGAGAGATTCCTTCCTCGGGAG
  ATGGACATCCTGGCAACTGTCAACCACGGCTCCATCATCAAGACTTACGAGATCTTTGAG
  ACCTCTGACGGACGGATCTACATCATCATGGAGCTTGGCGTCCAGGGCGACCTCCTCGAG
  TTCATCAAGTGCCAGGGAGCCCTGCATGAGGACGTGGCACGCAAGATGTTCCGACAGCTC
  TCCTCCGCCGTCAAGTACTGCCACGACCTGGACATCGTCCACCGGGACCTCAAGTGCGAG
  AACCTTCTCCTCGACAAGGACTTCAACATCAAGCTGTCTGACTTTGGCTTCTCCAAGCGC
  TGCCTGCGGGACAGCAATGGGCGCATCATCCTCAGCAAGACCTTCTGCGGGTCGGCAGCA
  TATGCAGCCCCCGAGGTGCTGCAGAGCATCCCCTACCAGCCCAAGGTGTATGACATCTGG
  AGCCTGGGCGTGATCCTGTACATCATGGTCTGCGGCTCCATGCCCTATGACGACTCCGAC
  ATCAGGAAGATGCTGCGTATCCAGAAGGAGCACCGTGTGGACTTCCCGCGCTCCAAGAAC
  CTGACCTGCGAGTGCAAGGACCTCATCTACCGCATGCTGCAGCCCGACGTCAGCCAGCGG
  CTCCACATCGATGAGATCCTCAGCCACTCGTGGCTGCAGCCCCCCAAGCCCAAAGCCACG
  TCTTCTGCCTCCTTCAAGAGGGAGGGGGAGGGCAAGTACCGCGCTGAGTGCAAACTGGAC
  ACCAAGACAGACTTGAGGCCCGACCACCGGCCCGACCACAAGCTTGGAGCCAAAACCCAG
  CACCGGCTGCTGGTGGTGCCCGAGAACGAGAACAGGATGGAGGACAGGCTGGCCGAGACC
  TCCAGGGCCAAAGACCATCACATCTCCGGAGCTGAGGTGGGGAAAGCAAGCACCTAG
  
  Clone variation with respect to NM_053006.4
  911 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_053006 unedited
  CCCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGA
  AGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGAGTCGAAGCCCAGCCCAGGGC
  AGCCCAGCCAGACGCCTCCGGTAGTGTAAATGAGGACAATGCCTGCTGGCCCACATGACGGGGGGATGTA
  GACGGCAGCGGCGCCAGTCGCTCCTGGCACCATGGACGATGCCACAGTCCTAAGGGAAGAGGGGTTACAT
  CGTAGGCATCAATCTTGGCAAGGGGTTCCTACGCAAAAGTCAAATCTGCCTACTCTGACCGCCCTCAGGT
  TAAAGTGGGTCTGGTCAGGATCCATCGACCGCAGAAAAAGAACCGCCTCAGGTTCATGGTGGCTGTAAGG
  ATCTCCGACCCAAGGAAATGGACCCCCCAAAGTCAATGGTGCGGTGTAAGAATATCGCGCCCCAAAGAAA
  TTAGAGGCCTCAAGTTTCCAATTGGGCTTGCTATTAATTCCGAACCGAAGAGAAATGGAACCGGCCCTCG
  TTTACTAGTTGGGGCTGACAATTACACGACCGCGAAAAAATGTGCGCCCCCAGTTTCAAGTGGGGCTGAT
  GAGATCCACACCGAGAAAACACCTCCTGTGATTGTGAGAAATCTCTCCTCCGGAGAGGAGGACTCGGACA
  GTGTCACCGCGCTCATATGAATATAAGATATTGTAACACTGAGAGGAGTGTACTCATATGAGATGTGGCT
  GGGACACTCAGTGATTAATGCTCGAGCGTCTGATGAAGGCTGCCAAAGTAGTCAGCATCCTCTCCTCCGA
  ATAGTACGACTGAGCTGTCGCAGAAGCAATGTGAACATTCTCTGAGCAGAGCTACCCTGGTGAATGAAGT
  
  Kinase domain sequence

  >SC323471 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGTAGCATCATCTTGGCAGGGTTCCTACGCAAAAGTCAAATCTGCCTACTCTGAGCGCCTCAAGTTCAAT
  GTGTCTGTCATGATCATCGACCGCAAGAAAATGAGCGCCTCAAGTTCAATGTGGCTGTCATGATCATCGA
  CCGCAAGAAAATGAGCGCCTCAAGTTCAATGTGGCTGTCAAGATCATCGACCGCAAGAAAATGAGCGCCT
  CAAGTTCAATGTGGCTGTCATGATCATCGACCGCAAGAAAATGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_053006
• ORF Size: 1077 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_053006.3, NP_443732.2
• RefSeq Size: 1363
• RefSeq ORF: 1077
• Locus ID: 23617
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]). [supplied by OMIM, Mar 2008]