ETS2 (NM_005239) Human Tagged ORF Clone

CAT#: RC207377

ETS2 (Myc-DDK-tagged)-Human v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) (ETS2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401607) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207377 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETS2
• Synonyms: ETS2IT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207377 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGATTTCGGAATCAAGAATATGGACCAGGTAGCCCCTGTGGCTAACAGTTACAGAGGGACACTCA
  AGCGCCAGCCAGCCTTTGACACCTTTGATGGGTCCCTGTTTGCTGTTTTTCCTTCTCTAAATGAAGAGCA
  AACACTGCAAGAAGTGCCAACAGGCTTGGATTCCATTTCTCATGACTCCGCCAACTGTGAATTGCCTTTG
  TTAACCCCGTGCAGCAAGGCTGTGATGAGTCAAGCCTTAAAAGCTACCTTCAGTGGCTTCAAAAAGGAAC
  AGCGGCGCCTGGGCATTCCAAAGAACCCCTGGCTGTGGAGTGAGCAACAGGTATGCCAGTGGCTTCTCTG
  GGCCACCAATGAGTTCAGTCTGGTGAACGTGAATCTGCAGAGGTTCGGCATGAATGGCCAGATGCTGTGT
  AACCTTGGCAAGGAACGCTTTCTGGAGCTGGCACCTGACTTTGTGGGTGACATTCTCTGGGAACATCTGG
  AGCAAATGATCAAAGAAAACCAAGAAAAGACAGAAGATCAATATGAAGAAAATTCACACCTCACCTCCGT
  TCCTCATTGGATTAACAGCAATACATTAGGTTTTGGCACAGAGCAGGCGCCCTATGGAATGCAGACACAG
  AATTACCCCAAAGGCGGCCTCCTGGACAGCATGTGTCCGGCCTCCACACCCAGCGTACTCAGCTCTGAGC
  AGGAGTTTCAGATGTTCCCCAAGTCTCGGCTCAGCTCCGTCAGCGTCACCTACTGCTCTGTCAGTCAGGA
  CTTCCCAGGCAGCAACTTGAATTTGCTCACCAACAATTCTGGGACGCCCAAAGACCACGACTCCCCTGAG
  AACGGTGCGGACAGCTTCGAGAGCTCAGACTCCCTCCTCCAGTCCTGGAACAGCCAGTCGTCCTTGCTGG
  ATGTGCAACGGGTTCCTTCCTTCGAGAGCTTCGAAGATGACTGCAGCCAGTCTCTCTGCCTCAATAAGCC
  AACCATGTCTTTCAAGGATTACATCCAAGAGAGGAGTGACCCGGTGGAGCAAGGCAAACCAGTTATACCT
  GCAGCTGTGCTGGCCGGCTTCACAGGAAGTGGACCTATTCAGCTGTGGCAGTTTCTCCTGGAGCTGCTAT
  CAGACAAATCCTGCCAGTCATTCATCAGCTGGACTGGAGACGGATGGGAGTTTAAGCTCGCCGACCCCGA
  TGAGGTGGCCCGCCGGTGGGGAAAGAGGAAAAATAAGCCCAAGATGAACTACGAGAAGCTGAGCCGGGGC
  TTACGCTACTATTACGACAAGAACATCATCCACAAGACGTCGGGGAAGCGCTACGTGTACCGCTTCGTGT
  GCGACCTCCAGAACTTGCTGGGGTTCACGCCCGAGGAACTGCACGCCATCCTGGGCGTCCAGCCAGACAC
  GGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207377 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNDFGIKNMDQVAPVANSYRGTLKRQPAFDTFDGSLFAVFPSLNEEQTLQEVPTGLDSISHDSANCELPL
  LTPCSKAVMSQALKATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLC
  NLGKERFLELAPDFVGDILWEHLEQMIKENQEKTEDQYEENSHLTSVPHWINSNTLGFGTEQAPYGMQTQ
  NYPKGGLLDSMCPASTPSVLSSEQEFQMFPKSRLSSVSVTYCSVSQDFPGSNLNLLTNNSGTPKDHDSPE
  NGADSFESSDSLLQSWNSQSSLLDVQRVPSFESFEDDCSQSLCLNKPTMSFKDYIQERSDPVEQGKPVIP
  AAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVARRWGKRKNKPKMNYEKLSRG
  LRYYYDKNIIHKTSGKRYVYRFVCDLQNLLGFTPEELHAILGVQPDTED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005239
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005239.1, NM_005239.2, NM_005239.3, NM_005239.4, NM_005239.5, NP_005230.1
• RefSeq Size: 3784 bp
• RefSeq ORF: 1410 bp
• Locus ID: 2114
• Cytogenetics: 21q22.2
• Domains: ETS, SAM_PNT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Dorso-ventral axis formation
• MW: 53 kDa
• Gene Summary: 'This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]'