Cyclin J (CCNJ) (NM_019084) Human Tagged ORF Clone

CAT#: RC207385

CCNJ (Myc-DDK-tagged)-Human cyclin J (CCNJ), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY412767) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCNJ
• Synonyms: bA690P14.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207385 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGGGGCAGTGGTGGCGAGGACAGCTGGCCGCCGATATTCACCAAGCGCTTCGCTACAAGG
  AGCTGAAGTTGCCCTCCTATAAAGGCCAGTCCCCTCAGTTAAGTCTCAGACGGTATTTTGCTGACTTGAT
  TGCCATTGTGAGCAATCGCTTCACACTCTGCCCTTCTGCCCGCCATCTTGCTGTCTATTTACTGGACCTG
  TTTATGGACCGCTATGACATCTCTATCCAGCAGCTGCATTTAGTTGCGCTTTCCTGCCTGCTTCTAGCAA
  GTAAATTTGAAGAAAAAGAAGACAGTGTGCCTAAGCTGGAGCAGCTCAACAGCCTGGGTTGTATGACTAA
  TATGAATCTAGTATTAACAAAACAAAATTTGCTACATATGGAACTATTATTATTAGAAACCTTTCAGTGG
  AACCTCTGCCTTCCAACAGCCGCCCATTTCATTGAGTATTATCTCTCTGAAGCAGTACACGAAACAGATC
  TTCATGACGGCTGGCCAATGATTTGCTTGGAAAAGACTAAACTCTACATGGCCAAATATGCAGATTACTT
  CCTGGAAGTATCTTTGCAAGTAGCTGCTGCATGTGTGGCTTCTTCGAGGATTATACTTCGTCTTTCTCCA
  ACGTGGCCTACAAGACTACATCGTCTTACTGCCTACTCTTGGGATTTCTTAGTGCAGTGTATTGAACGAC
  TGTTGATCGCTCATGATAATGATGTGAAAGAAGCAAACAAACAGAGAGGGCAAGCAGGACCTCAGTCAGC
  GCAACTAAGTGTATTCCAGACAGCCTCCCAGCCATCACGGCCAGTTCACTTTCAGCAACCTCAGTATCTC
  CATCAGACACATCAGACCTCACTGCAGTATCGCCATCCTACGTCAGAACAACCAAGCTGTCAGCAGATTG
  TATCGACCACACACACCTCATCTTACACACTACAGACATGTCCTGCTGGCTTCCAAACTAGTGTTCAGGG
  CCTTGGGCACATGCAGACTGGTGTTGGGATGTCACTGGCAATACCAGTAGAAGTTAAGCCCTGTCTGAGT
  GTTTCTTACAACCGGAGTTATCAGATAAATGAACATTACCCTTGTATTACTCCATGTTTTGAAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207385 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELEGQWWRGQLAADIHQALRYKELKLPSYKGQSPQLSLRRYFADLIAIVSNRFTLCPSARHLAVYLLDL
  FMDRYDISIQQLHLVALSCLLLASKFEEKEDSVPKLEQLNSLGCMTNMNLVLTKQNLLHMELLLLETFQW
  NLCLPTAAHFIEYYLSEAVHETDLHDGWPMICLEKTKLYMAKYADYFLEVSLQVAAACVASSRIILRLSP
  TWPTRLHRLTAYSWDFLVQCIERLLIAHDNDVKEANKQRGQAGPQSAQLSVFQTASQPSRPVHFQQPQYL
  HQTHQTSLQYRHPTSEQPSCQQIVSTTHTSSYTLQTCPAGFQTSVQGLGHMQTGVGMSLAIPVEVKPCLS
  VSYNRSYQINEHYPCITPCFER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019084
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019084.2, NM_019084.3, NM_019084.4, NP_061957.2
• RefSeq Size: 4120 bp
• RefSeq ORF: 1119 bp
• Locus ID: 54619
• Domains: cyclin_C
• MW: 42.6 kDa