FNDC4 (NM_022823) Human Tagged ORF Clone
CAT#: RC207471
FNDC4 (Myc-DDK-tagged)-Human fibronectin type III domain containing 4 (FNDC4)
"NM_022823" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FNDC4 |
Synonyms | FRCP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAGCGGATGCCACAGTTCCCCCCCCAGCGGACTCCGTGGGGACATGGCTTCGCTGGTGCCCCTTT CCCCATATCTAAGCCCCACGGTCCTCCTGCTGGTCAGCTGTGACCTGGGCTTCGTGCGAGCAGACCGGCC TCCCTCTCCTGTGAATGTGACGGTCACTCACCTCAGAGCCAACTCGGCCACTGTGTCCTGGGACGTCCCA GAAGGCAACATCGTCATTGGCTACTCCATTTCCCAGCAACGGCAGAATGGCCCCGGGCAGCGTGTGATTC GGGAGGTGAACACCACCACCCGGGCCTGTGCCCTCTGGGGCCTGGCTGAAGACAGTGACTACACAGTGCA GGTCAGGAGCATCGGCCTTCGGGGAGAGAGTCCCCCAGGGCCCCGGGTGCACTTCCGAACTCTCAAGGGT TCTGACCGGCTACCTTCAAACAGTTCAAGCCCAGGTGACATCACAGTGGAAGGTCTGGATGGAGAGCGGC CACTGCAGACTGGGGAAGTGGTCATCATTGTGGTGGTGTTGCTCATGTGGGCTGCTGTAATTGGGCTGTT CTGCCGTCAGTATGACATCATCAAGGACAATGACTCCAACAACAATCCCAAGGAGAAGGGAAAGGGGCCG GAACAGAGTCCTCAGGGAAGGCCAGTGGGGACAAGACAGAAAAAGTCACCATCTATCAACACCATCGACG TT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207471 protein sequence
Red=Cloning site Green=Tags(s) MPSGCHSSPPSGLRGDMASLVPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVP EGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKG SDRLPSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGP EQSPQGRPVGTRQKKSPSINTIDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022823 |
ORF Size | 702 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022823.1, NM_022823.2, NP_073734.1 |
RefSeq Size | 1697 bp |
RefSeq ORF | 705 bp |
Locus ID | 64838 |
Domains | FN3 |
Protein Families | Transmembrane |
MW | 25.2 kDa |
Gene Summary | Acts as an anti-inflammatory factor in the intestine and colon. Binds to and acts on macrophages to downregulate pro-inflammatory gene expression. Affects key macrophage functions, including phagocytosis, by downregulating many key pathways for macrophage activation, partly via by STAT3 activation and signaling. May be required to dampen the immunological response in colitis. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112412 | FNDC4 (untagged)-Human fibronectin type III domain containing 4 (FNDC4) |
USD 310.00 |
|
RG207471 | FNDC4 (GFP-tagged) - Human fibronectin type III domain containing 4 (FNDC4) |
USD 460.00 |
|
RC207471L3 | Lenti ORF clone of Human fibronectin type III domain containing 4 (FNDC4), Myc-DDK-tagged |
USD 620.00 |
|
RC207471L4 | Lenti ORF clone of Human fibronectin type III domain containing 4 (FNDC4), mGFP tagged |
USD 620.00 |
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