ACTL7B (NM_006686) Human Tagged ORF Clone

CAT#: RC207474

ACTL7B (Myc-DDK-tagged)-Human actin-like 7B (ACTL7B)

Product Images

Western blot validation of overexpression lysate (Cat# LY416488) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207474 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ACTL7B protein (Cat# TP307474). The protein was produced from HEK293T cells transfected with ACTL7B cDNA clone (Cat# RC207474) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACTL7B
• Synonyms: Tact1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207474 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACAAGGAACAGCCCCATGCCCCTGGGCACGGCTCAGGGTGACCCTGGAGAGGCAGGAACACGGC
  CCGGCCCTGACGCCAGCCTCCGGGACACAGGTGCGGCCACTCAGCTCAAGATGAAGCCCAGGAAGGTGCA
  CAAGATCAAGGCGGTCATCATCGACCTGGGCTCCCAGTACTGCAAGTGCGGCTACGCGGGAGAGCCGAGG
  CCCACCTACTTCATCTCCTCCACCGTGGGCAAACGCTGCCCCGAGGCGGCCGACGCTGGCGACACCCGCA
  AGTGGACTTTAGTGGGCCATGAGCTGCTCAACACGGAGGCGCCTCTCAAGCTGGTGAACCCGCTGAAGCA
  CGGCATCGTGGTGGACTGGGACTGCGTGCAGGACATCTGGGAGTACATCTTCCGCACCGCCATGAAGATC
  CTCCCCGAGGAGCACGCTGTGCTGGTCTCCGACCCTCCGCTCAGCCCCAGCAGCAACCGGGAGAAGTACG
  CGGAGCTCATGTTTGAGACCTTCGGCATCCCAGCCATGCACGTGACGTCCCAGTCGTTGCTGTCCATCTA
  CTCCTACGGCAAGACCTCGGGGCTGGTGGTGGAGAGCGGGCACGGCGTCTCGCACGTGGTGCCCATATCC
  GAGGGCGACGTGCTGCCGGGCCTGACCAGCCGCGCCGACTACGCTGGGGGTGACCTCACCAACTACCTGA
  TGCAGCTGCTCAATGAGGCGGGCCACGCATTCACGGACGACCACCTGCACATCATAGAGCACATCAAGAA
  GAAGTGCTGCTATGCGGCCTTCCTGCCCGAGGAGGAGCTGGGCCTGGTCCCGGAGGAGCTGCGCGTGGAC
  TACGAGCTCCCGGACGGCAAACTCATCACCATTGGCCAGGAGCGCTTCCGTTGCTCTGAGATGCTCTTCC
  AGCCCTCCCTGGCAGGCAGCACCCAGCCGGGCCTCCCGGAGCTCACAGCTGCCTGCCTGGGCCGCTGCCA
  GGACACGGGCTTCAAGGAGGAGATGGCCGCCAACGTGCTACTGTGTGGCGGCTGCACTATGCTGGATGGC
  TTCCCCGAGCGCTTCCAGAGGGAGCTGAGCCTCCTCTGCCCCGGGGACAGCCCTGCAGTGGCTGCCGCTC
  CTGAGAGGAAGACCTCCGTGTGGACCGGCGGTTCCATCCTGGCCTCCCTGCAGGCCTTCCAACAGCTCTG
  GGTCAGCAAGGAAGAGTTTGAGGAGCGGGGCAGCGTGGCCATCTACAGCAAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207474 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATRNSPMPLGTAQGDPGEAGTRPGPDASLRDTGAATQLKMKPRKVHKIKAVIIDLGSQYCKCGYAGEPR
  PTYFISSTVGKRCPEAADAGDTRKWTLVGHELLNTEAPLKLVNPLKHGIVVDWDCVQDIWEYIFRTAMKI
  LPEEHAVLVSDPPLSPSSNREKYAELMFETFGIPAMHVTSQSLLSIYSYGKTSGLVVESGHGVSHVVPIS
  EGDVLPGLTSRADYAGGDLTNYLMQLLNEAGHAFTDDHLHIIEHIKKKCCYAAFLPEEELGLVPEELRVD
  YELPDGKLITIGQERFRCSEMLFQPSLAGSTQPGLPELTAACLGRCQDTGFKEEMAANVLLCGGCTMLDG
  FPERFQRELSLLCPGDSPAVAAAPERKTSVWTGGSILASLQAFQQLWVSKEEFEERGSVAIYSKC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006686
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006686.1, NM_006686.2, NM_006686.3, NP_006677.1
• RefSeq Size: 1408 bp
• RefSeq ORF: 1248 bp
• Locus ID: 10880
• MW: 45.2 kDa
• Gene Summary: The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7B), and related gene, ACTL7A, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7B gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. Unlike ACTL7A, the ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. [provided by RefSeq, Jul 2008]