FCSK (NM_145059) Human Tagged ORF Clone

CAT#: RC207668

FUK (Myc-DDK-tagged)-Human fucokinase (FUK)

Product Images

Coomassie blue staining of purified FCSK protein (Cat# TP307668). The protein was produced from HEK293T cells transfected with FCSK cDNA clone (Cat# RC207668) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCSK
• Synonyms: 1110046B12Rik; CDGF2; FUK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207668 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGCCGAAGGGAGTTGATTGGACAGTCATCATCCTGACCTGCCAGTACAAGGACAGTGTCCAGG
  TCTTTCAGAGAGAACTGGAAGTGCGGCAGAAGCGGGAGCAGATCCCTGCTGGGACGCTGTTACTGGCCGT
  GGAGGACCCAGAGAAGCGTGTGGGCAGCGGAGGAGCCACCCTCAACGCCCTGCTGGTGGCTGCTGAACAC
  CTGAGTGCCCGGGCAGGCTTCACTGTGGTCACATCCGATGTCCTGCACTCGGCCTGGATCCTCATTCTGC
  ACATGGGTCGAGACTTCCCCTTTGATGACTGTGGCAGGGCTTTCACCTGCCTCCCCGTGGAGAACCCCGA
  GGCCCCCGTGGAAGCCTTGGTCTGCAACCTGGACTGCCTGCTGGACATCATGACCTATCGGCTGGGCCCG
  GGCTCCCCGCCAGGCGTGTGGGTCTGCAGCACCGACATGCTGCTGTCTGTTCCTGCAAATCCTGGTATCA
  GCTGGGACAGCTTCCGGGGAGCCAGAGTGATCGCCCTCCCAGGGAGCCCGGCCTACGCTCAGAATCATGG
  CGTCTACCTAACTGACCCCCAGGGCCTTGTTTTGGACATTTACTACCAGGGCACTGAGGCAGAGATTCAG
  CGGTGTGTCAGGCCTGATGGGCGGGTGCCACTGGTCTCTGGGGTTGTCTTCTTCTCTGTGGAGACTGCCG
  AGCGCCTCCTAGCCACCCACGTGAGCCCGCCCCTGGATGCCTGCACCTACCTAGGCTTGGACTCCGGAGC
  CCGGCCTGTCCAGCTGTCTCTGTTTTTTGACATTCTCCACTGCATGGCTGAGAACGTGACCAGGGAGGAC
  TTCCTGGTGGGGAGGCCCCCAGAGTTGGGGCAAGGCGATGCAGATGTAGCGGGTTATCTGCAGAGCGCCC
  GGGCCCAGCTGTGGAGGGAGCTTCGCGATCAGCCCCTTACCATGGCCTATGTCTCCAGCGGCAGCTACAG
  CTACATGACCTCCTCAGCCAGTGAGTTCCTGCTCAGCCTCACACTCCCCGGGGCTCCTGGGGCCCAGATT
  GTGCACTCCCAGGTGGAGGAGCAGCAGCTTCTGGCGGCCGGGAGCTCTGTGGTCAGCTGCCTGCTGGAGG
  GCCCTGTCCAGCTGGGTCCTGGGAGCGTCCTGCAGCACTGCCACCTGCAGGGCCCCATTCACATAGGCGC
  TGGCTGCTTGGTGACTGGCCTGGATACAGCCCACTCCAAGGCCCTGCATGGCCGGGAGCTGCGTGACCTT
  GTCCTGCAGGGACACCACACGCGGCTACACGGCTCCCCGGGCCACGCCTTCACCCTCGTTGGCCGTCTGG
  ACAGCTGGGAGAGACAGGGGGCAGGCACATATCTCAACGTGCCCTGGAGTGAATTCTTCAAGAGGACAGG
  TGTTCGAGCCTGGGACCTGTGGGACCCTGAGACGCTGCCCGCAGAGTACTGCCTTCCCAGCGCCCGCCTC
  TTTCCTGTGCTCCACCCCTCGAGGGAGCTGGGACCCCAGGACCTGCTGTGGATGCTGGACCACCAGGAGG
  ATGGGGGCGAGGCCCTGCGAGCCTGGCGGGCCTCCTGGCGCCTGTCCTGGGAGCAGCTGCAGCCGTGCCT
  GGATCGGGCTGCCACGCTGGCCTCTCGCCGGGACCTGTTCTTCCGCCAGGCCCTGCATAAGGCGCGGCAC
  GTGCTGGAGGCCCGGCAGGACCTCAGCCTGCGCCCGCTGATCTGGGCTGCTGTCCGCGAGGGCTGCCCCG
  GGCCCCTGCTGGCCACGCTGGACCAGGTTGCAGCTGGGGCAGGAGACCCTGGTGTGGCGGCACGGGCACT
  GGCCTGTGTGGCGGACGTCCTGGGCTGCATGGCAGAGGGCCGTGGGGGCTTGCGGAGCGGGCCAGCTGCC
  AACCCTGAGTGGATGCGGCCCTTCTCATACCTGGAGTGTGGAGACCTGGCAGCGGGCGTGGAGGCGCTTG
  CCCAGGAGAGGGACAAGTGGCTAAGCAGGCCAGCCTTGCTGGTGCGAGCGGCCCGCCACTATGAGGGGGC
  TGGTCAGATCCTGATCCGCCAGGCTGTGATGTCAGCCCAGCACTTTGTCTCCACAGAGCAGGTGGAACTG
  CCGGGACCTGGGCAGTGGGTGGTGGCTGAGTGCCCGGCCCGTGTGGATTTCTCTGGGGGCTGGAGTGACA
  CGCCACCCCTTGCCTATGAGCTTGGCGGGGCTGTGCTGGGCCTGGCTGTGCGAGTGGACGGCCGCCGGCC
  CATCGGAGCCAGGGCACGCCGCATCCCGGAGCCTGAGCTGTGGCTGGCGGTGGGGCCTCGGCAGGATGAG
  ATGACTGTGAAGATAGTGTGCCGGTGCCTGGCTGACCTGCGGGACTACTGCCAGCCTCATGCCCCAGGGG
  CCCTGCTGAAGGCGGCCTTCATCTGTGCAGGGATCGTGCATGTCCACTCGGAACTCCAGCTGAGTGAGCA
  GCTGCTCCGCACCTTCGGGGGCGGCTTTGAGCTGCACACCTGGTCTGAGCTGCCCCACGGCTCTGGCCTG
  GGCACCAGCAGCATCCTGGCAGGCACTGCCCTGGCTGCCTTGCAGCGAGCCGCAGGCCGGGTGGTGGGCA
  CGGAAGCCCTGATCCACGCAGTGCTGCACCTGGAGCAGGTGCTCACCACTGGAGGTGGCTGGCAGGACCA
  AGTAGGTGGCCTAATGCCTGGCATCAAGGTGGGGCGCTCCCGGGCTCAGCTGCCACTGAAGGTGGAGGTA
  GAAGAGGTCACGGTGCCTGAGGGCTTTGTCCAGAAGCTCAATGACCACCTGCTCTTGGTGTACACTGGCA
  AGACCCGCCTGGCTCGGAACCTGCTGCAGGATGTGCTGAGGAGCTGGTATGCCCGACTTCCTGCTGTGGT
  GCAGAATGCCCACAGCCTGGTACGGCAAACTGAGGAGTGTGCTGAAGGCTTCCGCCAAGGAAGCCTGCCT
  CTGCTGGGCCAGTGCCTGACCTCGTACTGGGAGCAGAAGAAGCTCATGGCTCCAGGCTGTGAGCCCCTGA
  CTGTGCGGCGTATGATGGATGTCCTGGCCCCCCACGTGCATGGCCAGAGCCTGGCTGGGGCAGGCGGTGG
  AGGCTTTCTCTATCTGTTGACCAAGGAGCCACAGCAAAAGGAGGCCTTGGAGGCGGTGCTGGCCAAGACC
  GAGGGCCTTGGGAATTACAGCATCCACCTGGTTGAAGTGGACACTCAGGGCCTGAGCCTGAAGCTGCTGG
  GGACCGAGGCCTCAACCTGTTGCCCTTTCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207668 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQPKGVDWTVIILTCQYKDSVQVFQRELEVRQKREQIPAGTLLLAVEDPEKRVGSGGATLNALLVAAEH
  LSARAGFTVVTSDVLHSAWILILHMGRDFPFDDCGRAFTCLPVENPEAPVEALVCNLDCLLDIMTYRLGP
  GSPPGVWVCSTDMLLSVPANPGISWDSFRGARVIALPGSPAYAQNHGVYLTDPQGLVLDIYYQGTEAEIQ
  RCVRPDGRVPLVSGVVFFSVETAERLLATHVSPPLDACTYLGLDSGARPVQLSLFFDILHCMAENVTRED
  FLVGRPPELGQGDADVAGYLQSARAQLWRELRDQPLTMAYVSSGSYSYMTSSASEFLLSLTLPGAPGAQI
  VHSQVEEQQLLAAGSSVVSCLLEGPVQLGPGSVLQHCHLQGPIHIGAGCLVTGLDTAHSKALHGRELRDL
  VLQGHHTRLHGSPGHAFTLVGRLDSWERQGAGTYLNVPWSEFFKRTGVRAWDLWDPETLPAEYCLPSARL
  FPVLHPSRELGPQDLLWMLDHQEDGGEALRAWRASWRLSWEQLQPCLDRAATLASRRDLFFRQALHKARH
  VLEARQDLSLRPLIWAAVREGCPGPLLATLDQVAAGAGDPGVAARALACVADVLGCMAEGRGGLRSGPAA
  NPEWMRPFSYLECGDLAAGVEALAQERDKWLSRPALLVRAARHYEGAGQILIRQAVMSAQHFVSTEQVEL
  PGPGQWVVAECPARVDFSGGWSDTPPLAYELGGAVLGLAVRVDGRRPIGARARRIPEPELWLAVGPRQDE
  MTVKIVCRCLADLRDYCQPHAPGALLKAAFICAGIVHVHSELQLSEQLLRTFGGGFELHTWSELPHGSGL
  GTSSILAGTALAALQRAAGRVVGTEALIHAVLHLEQVLTTGGGWQDQVGGLMPGIKVGRSRAQLPLKVEV
  EEVTVPEGFVQKLNDHLLLVYTGKTRLARNLLQDVLRSWYARLPAVVQNAHSLVRQTEECAEGFRQGSLP
  LLGQCLTSYWEQKKLMAPGCEPLTVRRMMDVLAPHVHGQSLAGAGGGGFLYLLTKEPQQKEALEAVLAKT
  EGLGNYSIHLVEVDTQGLSLKLLGTEASTCCPFP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145059
• ORF Size: 3252 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145059.2, NP_659496.2
• RefSeq Size: 3923
• RefSeq ORF: 3255
• Locus ID: 197258
• Domains: GHMP_kinases
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• MW: 117.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq, Jul 2008]