AFAP1L2 (NM_001001936) Human Tagged ORF Clone

CAT#: RC207705

AFAP1L2 (Myc-DDK-tagged)-Human actin filament associated protein 1-like 2 (AFAP1L2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424282) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207705 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AFAP1L2 protein (Cat# TP307705). The protein was produced from HEK293T cells transfected with AFAP1L2 cDNA clone (Cat# RC207705) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFAP1L2
• Synonyms: CTB-1144G6.4; KIAA1914; XB130
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207705 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTACAAAGCCCTGGAACAGCTGCTGACAGAGTTGGATGACTTCCTCAAGATTCTTGACCAGG
  AGAACCTGAGCAGCACAGCACTGGTGAAGAAGAGCTGCCTGGCGGAGCTCCTCCGGCTTTACACCAAAAG
  CAGCAGCTCTGATGAGGAGTACATTTATATGAACAAAGTGACCATCAACAAGCAACAGAATGCAGAGTCT
  CAAGGCAAAGCGCCTGAGGAGCAGGGCCTGCTACCCAATGGGGAGCCCAGCCAGCACTCCTCGGCCCCTC
  AGAAGAGCCTTCCAGACCTCCCGCCACCCAAGATGATTCCAGAACGGAAACAGCTTGCCATCCCAAAGAC
  GGAGTCTCCAGAGGGCTACTATGAAGAGGCTGAGCCATATGACACATCCCTCAATGAGGACGGAGAGGCT
  GTGAGCAGCTCCTACGAGTCCTACGATGAAGAGGACGGCAGCAAGGGCAAGTCGGCCCCTTACCAGTGGC
  CCTCGCCGGAGGCCGGCATCGAGCTGATGCGTGACGCCCGCATCTGCGCCTTCCTGTGGCGCAAGAAGTG
  GCTGGGACAGTGGGCCAAGCAGCTCTGTGTCATCAAGGACAACAGGCTTCTGTGCTACAAATCCTCCAAG
  GACCACAGCCCTCAGCTGGACGTGAACCTACTGGGCAGCAGCGTCATTCACAAGGAGAAGCAAGTGCGGA
  AGAAGGAGCACAAGCTGAAGATCACACCGATGAATGCCGATGTGATTGTGCTGGGCCTGCAGAGCAAGGA
  CCAGGCTGAGCAGTGGCTCAGGGTCATCCAGGAAGTGAGCGGCCTGCCTTCCGAAGGAGCATCTGAAGGA
  AACCAGTACACCCCGGATGCCCAGCGCTTTAACTGCCAGAAACCAGATATAGCTGAGAAGTACCTGTCGG
  CTTCAGAGTATGGGAGCTCCGTGGATGGCCACCCTGAGGTCCCAGAAACCAAAGACGTCAAGAAGAAATG
  TTCTGCTGGCCTCAAACTGAGCAACCTAATGAATCTGGGCAGGAAGAAATCCACCTCACTGGAGCCTGTG
  GAGAGGTCCCTCGAGACATCCAGTTACCTGAACGTGCTGGTGAACAGCCAGTGGAAGTCTCGCTGGTGCT
  CTGTCAGGGACAATCACCTGCACTTCTACCAGGACCGGAACCGGAGCAAGGTGGCCCAGCAACCCCTCAG
  CCTGGTGGGCTGCGAGGTGGTCCCAGACCCCAGCCCCGACCACCTCTACTCCTTCCGCATCCTCCACAAG
  GGCGAGGAGCTGGCCAAGCTTGAGGCCAAGTCTTCCGAGGAAATGGGCCACTGGCTGGGTCTCCTGCTCT
  CTGAGTCAGGCTCCAAGACAGACCCAGAAGAGTTCACCTACGACTATGTGGATGCCGATAGGGTCTCCTG
  TATTGTGAGTGCGGCCAAAAACTCTCTCTTACTGATGCAGAGAAAGTTCTCAGAGCCCAACACTTACATC
  GATGGCCTGCCTAGCCAGGACCGCCAGGAGGAGCTGTATGACGACGTGGACCTGTCAGAGCTCACAGCTG
  CGGTGGAGCCTACCGAGGAAGCCACCCCTGTTGCAGATGACCCAAATGAGAGAGAATCTGACCGGGTGTA
  CCTGGACCTCACACCTGTCAAGTCCTTTCTGCATGGCCCCAGCAGTGCACAGGCCCAGGCCTCCTCCCCG
  ACGTTGTCCTGCCTGGACAATGCAACTGAGGCCCTCCCGGCAGACTCAGGCCCAGGTCCCACCCCAGATG
  AGCCCTGCATAAAGTGTCCAGAGAACCTGGGAGAACAGCAGCTGGAGAGTTTGGAGCCAGAGGATCCTTC
  CCTGAGAATCACCACCGTCAAAATCCAGACGGAACAGCAGAGAATCTCCTTCCCACCGAGCTGCCCGGAT
  GCCGTGGTGGCCACCCCACCTGGTGCCAGCCCACCTGTGAAGGACAGGTTGCGCGTGACCAGTGCAGAGA
  TCAAGCTTGGCAAGAATCGGACAGAAGCTGAGGTGAAGCGGTACACAGAGGAGAAGGAGAGGCTTGAAAA
  GAAGAAGGAAGAAATCCGGGGGCACCTGGCTCAGCTCCGGAAAGAGAAACGGGAGCTAAAGGAAACCCTA
  CTGAAATGCACAGACAAGGAAGTCCTGGCGAGCCTGGAGCAGAAGCTGAAGGAAATTGACGAGGAGTGCC
  GGGGCGAGGAGAGCAGGCGCGTGGACCTGGAGCTCAGCATCATGGAGGTGAAGGACAACCTGAAGAAGGC
  TGAGGCAGGGCCTGTGACGTTAGGCACCACCGTGGACACCACCCACCTGGAGAATGTGAGCCCCCGCCCC
  AAAGCTGTCACACCTGCCTCTGCCCCAGACTGTACCCCAGTCAACTCTGCAACCACACTCAAGAACAGGC
  CTCTCTCGGTCGTGGTCACAGGCAAAGGCACTGTACTCCAGAAAGCCAAGGAATGGGAGAAGAAAGGAGC
  AAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207705 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERYKALEQLLTELDDFLKILDQENLSSTALVKKSCLAELLRLYTKSSSSDEEYIYMNKVTINKQQNAES
  QGKAPEEQGLLPNGEPSQHSSAPQKSLPDLPPPKMIPERKQLAIPKTESPEGYYEEAEPYDTSLNEDGEA
  VSSSYESYDEEDGSKGKSAPYQWPSPEAGIELMRDARICAFLWRKKWLGQWAKQLCVIKDNRLLCYKSSK
  DHSPQLDVNLLGSSVIHKEKQVRKKEHKLKITPMNADVIVLGLQSKDQAEQWLRVIQEVSGLPSEGASEG
  NQYTPDAQRFNCQKPDIAEKYLSASEYGSSVDGHPEVPETKDVKKKCSAGLKLSNLMNLGRKKSTSLEPV
  ERSLETSSYLNVLVNSQWKSRWCSVRDNHLHFYQDRNRSKVAQQPLSLVGCEVVPDPSPDHLYSFRILHK
  GEELAKLEAKSSEEMGHWLGLLLSESGSKTDPEEFTYDYVDADRVSCIVSAAKNSLLLMQRKFSEPNTYI
  DGLPSQDRQEELYDDVDLSELTAAVEPTEEATPVADDPNERESDRVYLDLTPVKSFLHGPSSAQAQASSP
  TLSCLDNATEALPADSGPGPTPDEPCIKCPENLGEQQLESLEPEDPSLRITTVKIQTEQQRISFPPSCPD
  AVVATPPGASPPVKDRLRVTSAEIKLGKNRTEAEVKRYTEEKERLEKKKEEIRGHLAQLRKEKRELKETL
  LKCTDKEVLASLEQKLKEIDEECRGEESRRVDLELSIMEVKDNLKKAEAGPVTLGTTVDTTHLENVSPRP
  KAVTPASAPDCTPVNSATTLKNRPLSVVVTGKGTVLQKAKEWEKKGAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001936
• ORF Size: 2454 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001936.1, NM_001001936.2, NP_001001936.1
• RefSeq Size: 4019 bp
• RefSeq ORF: 2457 bp
• Locus ID: 84632
• MW: 91.3 kDa