KCTD10 (NM_031954) Human Tagged ORF Clone

CAT#: RC207723

KCTD10 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 10 (KCTD10)

Product Images

Western blot validation of overexpression lysate (Cat# LY410399) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207723 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCTD10 protein (Cat# TP307723). The protein was produced from HEK293T cells transfected with KCTD10 cDNA clone (Cat# RC207723) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD10
• Synonyms: BTBD28; hBACURD3; MSTP028; ULRO61
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207723 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGCACCACTTCCT
  TCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGAGCCCTCTACTATACCACCAT
  GCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATGTTCAGCGGGCGCATGGAAGTGCTCACCGAC
  AGTGAAGGCTGGATCCTCATTGACCGCTGTGGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACG
  GGGCGGTGCCTTTACCCGAGAGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGT
  CCAAGGCCTGGTGGAAGAGTGCCAGGCGGCCCTACAAAACAAAGATACTTATGAGCCTTTCTGCAAGGTC
  CCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAGCCAGCCGTGAAGTTGC
  TCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCTGACGACAATATGTTGAAAAACATTGA
  ACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGAAGGGTCCTGTTCATAAAGGATGTCATTGGGGATGAA
  ATCTGCTGCTGGTCCTTTTATGGTCAGGGCCGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATG
  CCACTGAGAAGAAACAGACCAAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTT
  GCTGTATGAGGCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCGGCGGGG
  CGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGGAGGATCCACATCAAGC
  GCCCTGATGACCGGGCCCACCTCCACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207723 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEMSGESVVSSAVPAAATRTTSFKGTSPSSKYVKLNVGGALYYTTMQTLTKQDTMLKAMFSGRMEVLTD
  SEGWILIDRCGKHFGTILNYLRDGAVPLPESRREIEELLAEAKYYLVQGLVEECQAALQNKDTYEPFCKV
  PVITSSKEEQKLIATSNKPAVKLLYNRSNNKYSYTSNSDDNMLKNIELFDKLSLRFNGRVLFIKDVIGDE
  ICCWSFYGQGRKIAEVCCTSIVYATEKKQTKVEFPEARIYEETLNILLYEAQDGRGPDNALLEATGGAAG
  RSHHLDEDEERERIERVRRIHIKRPDDRAHLHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031954
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031954.1, NM_031954.2, NM_031954.3, NM_031954.4, NP_114160.1
• RefSeq Size: 4057 bp
• RefSeq ORF: 942 bp
• Locus ID: 83892
• Domains: BTB, K_tetra
• Protein Families: Ion Channels: Other
• MW: 35.4 kDa
• Gene Summary: The protein encoded by this gene binds proliferating cell nuclear antigen (PCNA) and may be involved in DNA synthesis and cell proliferation. In addition, the encoded protein may be a tumor suppressor. Several protein-coding and non-protein coding transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]